常咯啉在实验性心律失常狗的药代动力学-药效动力学分析

用Harris冠脉结扎法诱发的心律失常狗研究常咯啉药代动力学-药效动力学。7只狗按83.33μg·kg^-1·min^-1静脉滴注60min,在给药期间和停药后不同时间记录ECG及测定血药浓度。C-T数据用药代程序计算药代参数;药效数据用药代-药效同步分析模型计算药效动力学参数,K₁₀, T_1/2,Vd,Cl分别为0.0087min^-1,78.03min,40.55ml·kg^-1和0.421ml·kg^-1·min^-1;K_eO和Ce(50)分别为0.0048min^-1和2.01μg·ml^-1.     

干燥综合征神经系统病变的临床及病理分析

目的探讨原发性干燥综合征(Sjogren综合征;pSS)神经系统病变的临床及组织病理特征。方法对18例并发神经系统损伤的pSS患者的临床及病理资料进行回顾性分析。结果周围神经系统病变pSS(PNS-pSS)表现为对称性感觉运动性周围神经病、多发性单神经病、感觉性周围神经病、颅神经损伤。中枢神经系统病变pSS(CNS-pSS)表现为多发性硬化、缓慢进展的认知障碍和共济障碍、脑炎。头颅MRI显示脑白质多发异常信号、脑白质、脑干及小脑多发异常信号、脑脓肿。唇黏膜活检示部分腺体萎缩,腺泡及间质内可见淋巴、单核吞噬细胞浸润。腓肠神经活检既有典型血管炎改变也有非血管炎性改变。脑活检可见白质脱髓鞘及小静脉周围炎性细胞浸润以及典型血管炎改变。结论Sjogren综合征导致的神经系统损伤呈多样性,且常先于Sjogren综合征的诊断之前出现。了解其神经系统损伤的临床表现、影像学特点及组织病理改变对认识本病具有重要的临床意义。     

临床感染病原菌菌种分布及其耐药性分析

目的了解笔者所在医院2008年度临床感染病原菌的菌种分布及其耐药现状。方法用VITEK微生物自动鉴定仪鉴定菌种,纸片扩散法检测临床分离菌对各种抗菌药物的敏感性,判读标准参照当年的CLSI M100文件。结果临床分离的1900株病原菌中革兰阴性杆菌、革兰阳性球菌和真菌所占比例分别为68.42%、18.16%和12.32%。分离率最高的五种病原菌:铜绿假单胞菌、大肠埃希菌、肺炎克雷伯菌、鲍曼不动杆菌和白色假丝酵母菌,占所有分离病原菌的59.26%,排前20位的病原菌则占到近90%(89.63%)。革兰阴性杆菌仅对亚胺培南、头孢哌酮/舒巴坦、美洛培南、哌拉西林/他唑巴坦的平均耐药率低于30%,铜绿假单胞菌和鲍曼不动杆菌对亚胺培南、美洛培南的耐药率分别28.9%、32.9%和11.6%、24.7%,大肠埃希菌、肺炎克雷伯菌和奇异变形杆菌的ESBL阳性率分别是52.8%、37.2%和34.6%,金黄色葡萄球和凝固酶阴性葡萄球菌中耐甲氧西林株各占44.0%和60.0%,未检出万古霉素中介或耐药的葡萄球菌,检出万古霉素耐药的粪肠球菌和屎肠球菌各1株。念珠菌对氟康唑耐药率接近5%。结论临床感染病原菌构成变化不大,但真菌检出率逐年升高。非发酵菌耐药率最低的是头孢哌酮/舒巴坦,肠杆菌科耐药率最低的是亚胺培南,ESBL的阳性率逐年升高。加强病原菌及其耐药性检测和监测,对病原学诊断及指导合理选用抗生素具重要参考价值。     

机械通气患者中心静脉压与上腔静脉横径相关性研究

目的通过监测机械通气患者中心静脉压(CVP)与上腔静脉横径值(SVCD)的动态变化,评价呼气末正压(PEEP)对机械通气患者CVP与SVCD的影响,以及CVP与SVCD相关性研究,为用无创手段测定机械通气患者SVCD值以来指导患者液体治疗提供理论依据。方法选取22例因急性呼吸衰竭行机械通气的患者应用彩色超声诊断仪监测受试者在不同水平PEEP(0～20cmH2O)时的SVCD值与CVP值,同时记录患者心率、平均动脉压与血氧饱和度,分析SVCD、CVP与PEEP相关性分析。结果①机械通气患者SVCD值随PEEP值的增加而递减,SVCD与PEEP值呈显著负相关(r=-0.981,P<0.01);②CVP与PEEP呈显著正相关(r=0.997,P<0.01);③CVP与SVCD值呈显著负相关(r=-0.961,P<0.01)。结论机械通气时患者SVCD值随PEEP值增加而减少,CVP随PEEP值增加而增加,从而可用多普勒超声心动图检查来监测SVCD值,以间接评价患者治疗效果。     

Cryptosporidium Spp. Infection in Human and Domestic Animals

Background

Cryptosporidium spp. is a coccidian parasite infected humans and animals. Prevalence rate of Cryptosporidium spp. infection associated with is some parameters such as sampling, age, season, country and contact to domestic animals. This study aimed to determine Cryptosporidium spp. Infection in humans and some animals in rural areas of Shushtar district from Khuzestan Province, south- west of Iran.

Methods

In this study, Stool specimens were randomly collected from 45 cattle, 8 buffalos, 35 calves, 22 turkeys, 3 sheep, 2 geese as well as 62 humans in different seasons selected from rural areas of Shushtar district located in Khuzestan in the south- west of Iran from August 2009 to April 2011. The collected stool samples were examined by modified Ziehl-Neelsen staining method.

Results

Altogether, 68/115 (59.1%) domestic animals and 9/62 (14.5%) of humans were showed Cryptosporidium spp. infection in the study areas.

Conclusion

In this study we found the high frequency of Cryptosporidium spp. infection in the studied areas.     

Novel in Vitro Efficiency of Chitosan Biomolecule against Trichomonas gallinae

Background

Development of new natural agents for parasitic diseases treatment has unexpectedly increased to overcome effectively against emergence and re-emergence of parasitic diseases, the appearance of drug resistant organisms and toxic side effects of current agents. The aim of the study was to evaluate antiprotozoal activities of chitosan biomolecule on trophozoites of Trichomonas gallinae.

Methods

The antitrichomonal activity of various low molecular weight chitosan concentrations including 125, 250, 500 and 1250 µg ml⁻¹ against T. gallinae trophozoites cultured in trypticase-yeast extract-maltose medium supplemented with heat-inactivated cold horse serum was evaluated in vitro. Samples containing medium without chitosan were also assayed as controls.

Results

The mortality rates at 0, 3 and 6 h post treatment with all concentrations were significantly different from control group (P<0.05). Treated trophozoites showed more susceptibility to the highest concentration reaching mortality rate of 100% at 3h post inoculation. However, at this time, results for 125, 250 and 500 µg ml⁻¹ were 93%, 95% and 96.7%, respectively.

Conclusion

The results demonstrate that the application of chitosan biomolecule is a promising option for treatment of trichomoniasis in pigeons.     

The Role of Serotonin (5-HT) in Behavioral Control: Findings from Animal Research and Clinical Implications

The neurotransmitters serotonin and dopamine both have a critical role in the underlying neurobiology of different behaviors. With focus on the interplay between dopamine and serotonin, it has been proposed that dopamine biases behavior towards habitual responding, and with serotonin offsetting this phenomenon and directing the balance toward more flexible, goal-directed responding. The present focus paper stands in close relationship to the publication by Worbe et al. (2015), which deals with the effects of acute tryptophan depletion, a neurodietary physiological method to decrease central nervous serotonin synthesis in humans for a short period of time, on the balance between hypothetical goal-directed and habitual systems. In that research, acute tryptophan depletion challenge administration and a following short-term reduction in central nervous serotonin synthesis were associated with a shift of behavioral performance towards habitual responding, providing further evidence that central nervous serotonin function modulates the balance between goal-directed and stimulus-response habitual systems of behavioral control. In the present focus paper, we discuss the findings by Worbe and colleagues in light of animal experiments as well as clinical implications and discuss potential future avenues for related research.     

美托洛尔治疗右室流出道室性早搏的效果观察

目的：观察美托洛尔对起源于右室流出道室性早搏的疗效。方法：选择起源于右室流出道的室性早搏患25例，给予美托洛尔治疗，剂量从6．25mg开始，无不良反应后，逐渐加量，最大剂量每天100－150mg，疗程3个月，用药前后查12导联体表心电图、24小时动态心电图及结合临床症状进行评定。结果：①临床症状评定：胸闷心悸25例，治疗后减轻20例，有效率为80％；焦虑20例，治疗后症状均消失，有效率为100％；头晕14例，治疗后减轻12例，有效率为85．7％。②室性早搏评定：25例中达到室性早搏抑制率70％有11例，有效率为44％；平均室性早搏抑制率为45．6％，6例成对室性早搏抑制率为80％，3例短阵室速消失，平均心率降低12．6／min。③生化指标：血糖、胆固醇、甘油、三酯治疗前后无明显改变。④2例因血压低未加药量。结论：美托洛尔治疗起源于右室流出道室性早搏疗效肯定，尤其对运动后早搏增多疗效较好，临床太改善较显，观察中未发现明显不良反应，无致心律失常现象。     

Effect of medial arch-heel support in inserts on reducing ankle eversion: a biomechanics study

Background  

Excessive pronation (or eversion) at ankle joint in heel-toe running correlated with lower extremity overuse injuries. Orthotics and inserts are often prescribed to limit the pronation range to tackle the problem. Previous studies revealed that the effect is product-specific. This study investigated the effect of medial arch-heel support in inserts on reducing ankle eversion in standing, walking and running.