全文获取类型
收费全文 | 614篇 |
免费 | 37篇 |
国内免费 | 12篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 26篇 |
妇产科学 | 15篇 |
基础医学 | 67篇 |
口腔科学 | 12篇 |
临床医学 | 54篇 |
内科学 | 204篇 |
皮肤病学 | 15篇 |
神经病学 | 59篇 |
特种医学 | 11篇 |
外科学 | 62篇 |
综合类 | 1篇 |
预防医学 | 46篇 |
眼科学 | 15篇 |
药学 | 30篇 |
中国医学 | 1篇 |
肿瘤学 | 39篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 16篇 |
2021年 | 30篇 |
2020年 | 21篇 |
2019年 | 31篇 |
2018年 | 23篇 |
2017年 | 25篇 |
2016年 | 22篇 |
2015年 | 25篇 |
2014年 | 41篇 |
2013年 | 47篇 |
2012年 | 71篇 |
2011年 | 62篇 |
2010年 | 36篇 |
2009年 | 26篇 |
2008年 | 37篇 |
2007年 | 33篇 |
2006年 | 36篇 |
2005年 | 30篇 |
2004年 | 15篇 |
2003年 | 5篇 |
2002年 | 8篇 |
2001年 | 2篇 |
2000年 | 7篇 |
1998年 | 3篇 |
1985年 | 1篇 |
排序方式: 共有663条查询结果,搜索用时 0 毫秒
61.
Truncation of the Catalytic Domain of the Cylindromatosis Tumor Suppressor Impairs Lung Maturation
下载免费PDF全文
![点击此处可从《Neoplasia (New York, N.Y.)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Eirini Trompouki Ageliki Tsagaratou Stylianos K Kosmidis Pascal Doll Jun Qian Dimitris L Kontoyiannis Wellington V Cardoso George Mosialos 《Neoplasia (New York, N.Y.)》2009,11(5):469-476
Cyld encodes a 956-amino acid deubiquitinating enzyme (CYLD), which is a negative regulator of nuclear factor κB and mitogen-activated protein kinase pathways. Mutations that truncate and inactivate the carboxyl-terminal deubiquitinating domain of CYLD underlie the development of skin appendage tumors in humans, whereas down-regulation of Cyld expression has been associated with the development of various types of human malignancies including lung cancer. To establish an animal model of human CYLD inactivation and characterize the biological role of CYLD in vivo, we generated mice carrying a homozygous deletion of Cyld exon 9 (CyldΔ9/Δ9 mice) using a conditional approach. Deletion of exon 9 would cause a carboxyl-terminal truncation of CYLD and inactivation of its deubiquitinating activity. In accordance with previous studies, fibroblasts from CyldΔ9/Δ9 embryos had hyperactive nuclear factor κB and c-Jun kinase pathways compared with control fibroblasts. CyldΔ9/Δ9 newborn mice were smaller than wild-type littermates with a short and kinky tail and nomajor developmental defects. However, CyldΔ9/Δ9 mice died shortly after birth from apparent respiratory dysfunction. Histological examination of E18.5 CyldΔ9/Δ9 lungs demonstrated an immature phenotype characterized by hyperplasic mesenchyme but apparently normal epithelial, smooth muscle. and endothelial structures. Our study identifies an important role of CYLD in lung maturation, which may underlie the development of many cases of lung cancer. 相似文献
62.
Eirini Liodaki Philipp-Alexander Bergmann Konstantinos Kalousis Peter Mailänder Frank Siemers 《Breast (Edinburgh, Scotland)》2013,22(1):74-77
Nipple-areola complex (NAC) reconstruction tends to be the final phase of post-mastectomy reconstruction for many cancer patients, as it transforms the amorphous breast mound into a more aesthetically realistic breast. A variety of local-flap based techniques have been reported. In this paper we will describe a cantral-pedicled intracorial skin flap technique. Review of 12 patients showed aesthetic pleasing NAC and durable long-term results of nipple projection. 相似文献
63.
64.
Paterson L Papagiakoumou E Milne G Garcés-Chávez V Briscoe T Sibbett W Dholakia K Riches AC 《Journal of biomedical optics》2007,12(5):054017
A passive, optical cell sorter is created using the light pattern of a 'nondiffracting' beam-the Bessel beam. As a precursor to cell sorting studies, microspheres are used to test the resolution of the sorter on the basis of particle size and refractive index. Variations in size and, more noticeably, refractive index, lead to a marked difference in the migration time of spheres in the Bessel beam. Intrinsic differences (size, refractive index) between native (unlabeled) cell populations are utilized for cell sorting. The large difference in size between erythrocytes and lymphocytes results in their successful separation in this beam pattern. The intrinsic differences in size and refractive index of other cells in the study (HL60 human promyelocytic leukaemic cells, murine bone marrow, and murine stem/progenitor cells) are not large enough to induce passive optical separation. Silica microsphere tags are attached to cells of interest to modify their size and refractive index, resulting in the separation of labeled cells. Cells collected after separation are viable, as evidenced by trypan blue dye exclusion, their ability to clone in vitro, continued growth in culture, and lack of expression of Caspase 3, a marker of apoptosis. 相似文献
65.
Meletios A Dimopoulos Evangelos Terpos Mario Boccadoro Sosana Delimpasi Meral Beksac Eirini Katodritou Philippe Moreau Luca Baldini Argiris Symeonidis Jelena Bila Albert Oriol Maria-Victoria Mateos Hermann Einsele Ioannis Orfanidis Tahamtan Ahmadi Jon Ukropec Tobias Kampfenkel Jordan M Schecter Sonja Zweegman 《The lancet oncology》2021,22(6):801-812
66.
Lymph nodes play a central role in the development of adaptive immunity against pathogens and particularly the generation of antigen-specific B cell responses in specialized areas called germinal centers (GCs). Lymph node (LN) pathology was recognized as an important consequence of human immunodeficiency virus (HIV) infection since the beginning of the HIV epidemic. Investigation into the structural and functional alterations induced by HIV and Simian immunodeficiency virus (SIV) has further cemented the central role that lymphoid tissue plays in HIV/SIV pathogenesis. The coexistence of constant local inflammation, altered tissue architecture, and relative exclusion of virus-specific CD8 T cells from the GCs creates a unique environment for the virus evolution and establishment of viral reservoir in specific GC cells, namely T follicular helper CD4 T cells (Tfh). A better understanding of the biology of immune cells in HIV-infected lymph nodes is a prerequisite to attaining the ultimate goal of complete viral eradication. 相似文献
67.
68.
Nikolaos Spantideas Eirini Drosou Anastasia Bougea Dimitrios Assimakopoulos 《BMC ear, nose, and throat disorders》2015,15(1):1-10
Gait function may be impaired in patients with vestibular disorders, making gait assessment in the clinical setting relevant for this patient population. The purpose of this study was to evaluate the discriminant validity of a gait assessment protocol between patients with vestibular disorders and healthy participants. Furthermore, test re-test reproducibility and the measurement error of gait performance measures in patients with vestibular lesions was performed under different walking conditions. Gait parameters of thirty-five patients with vestibular disorders and twenty-seven healthy controls were assessed twice with the GAITRite® system. Discriminant validity, reproducibility (intra class correlation [ICC]) and the measurement error (standard error of measurement [SEM], smallest detectable change [SDC]) were determined for gait speed, cadence and step length. Bland-Altman plots were made to assess systematic bias between tests. A significant effect of grouping on gait performance indicates discriminant validity of gait assessment. All tests revealed differences between patients and healthy controls (p < 0.01). The ICCs for test re-test reproducibility were excellent (0.70-0.96) and measurement error showed acceptable SDC values for gait parameters derived from three walking conditions (9-19 %). Bland-Altman plots indicated no systematic bias. Good validity and reproducibility of GAITRite® system measurements suggest that this system could facilitate the study of gait in patients with vestibular disorders in clinical settings. The SDC values for gait are generally small enough to detect changes after a rehabilitation program for patients with vestibular disorders. 相似文献
69.
Eirini Athanasiadou Maria Paschalidou Anna Theocharidou Nikolaos Kontoudakis Konstantinos Arapostathis Athina Bakopoulou 《Dental materials》2018,34(12):1797-1813
Objective
To investigate the biological interactions of a calcium silicate based cement (Biodentine?) with Stem Cells from Human Exfoliated Deciduous teeth (SHED), focusing on viability/proliferation, odontogenic differentiation, biomineralization and elemental release/exchange.Methods
Biodentine? specimens were used directly or for eluate preparation at serial dilutions (1:1–1:64). SHED cultures were established from deciduous teeth of healthy children. Viability/proliferation and morphological characteristics were evaluated by live/dead fluorescent staining, MTT assay and Scanning Electron Microscopy. Odontogenic differentiation by qRT-PCR, biomineralization by Alizarin red S staining, while ion elution by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES).Results
SHED effectively attached within the crystalline surface of Biodentine? specimens acquiring a spindle-shaped phenotype. Statistically significant stimulation of cell proliferation was induced at day 3 by eluates in dilutions from 1:16 to 1:64. Differential, concentration- and time-dependent expression patterns of odontogenic genes were observed under non-inductive and inductive (osteogenic) conditions, with significant up-regulation of DSPP and Runx2 at higher dilutions and a peak in expression of BMP-2, BGLAP and MSX-2 at 1:8 dilution on day 7. Progressive increase in mineralized tissue formation was observed with increasing dilutions of Biodentine? eluates. ICP-OES indicated that Biodentine? absorbed Ca, Mg and P ions from culture medium, while releasing Si and Sr ions from its backbone.Significance
Biodentine? interacts through elemental release/uptake with the cellular microenvironment, triggering odontogenic differentiation and biomineralization in a concentration-dependent manner. These results reveal a promising strategy for application of the calcium silicate based cement (Biodentine?) for vital pulp therapies of deciduous teeth in Paediatric Dentistry. 相似文献70.
Christine I. Vaitsopoulou Efstratios M. Kolibianakis Julia K. Bosdou Eirini Neofytou Stefania Lymperi Anastasios Makedos Despina Savvaidou Katerina Chatzimeletiou Grigoris F. Grimbizis Alexandros Lambropoulos Basil C. Tarlatzis 《Reproductive biomedicine online》2021,42(1):248-259
Research questionSex hormone-binding globulin (SHBG), androgen receptor (AR), LH beta polypeptide (LHB), progesterone receptor membrane component 1 (PGRMC1) and progesterone receptor membrane component 2 (PGRMC2) regulate follicle development and maturation. Their mRNA expression was assessed in peripheral blood mononuclear cells (PBMC) of normal and poor responders, during ovarian stimulation.DesignFifty-two normal responders and 15 poor responders according to the Bologna criteria were enrolled for IVF and intracytoplasmic sperm injection and stimulated with 200 IU of follitrophin alpha and gonadotrophin-releasing hormone antagonist. HCG was administered for final oocyte maturation. On days 1, 6 and 10 of stimulation, blood samples were obtained, serum hormone levels were measured, RNA was extracted from PBMC and real-time polymerase chain reaction was carried out to identify the mRNA levels. Relative mRNA expression of each gene was calculated by the comparative 2?DDCt method.ResultsDifferences between mRNA levels of each gene on the same time point between the two groups were not significant. PGRMC1 and PGRMC2 mRNA levels were downregulated, adjusted for ovarian response and age. Positive correlations between PGRMC1 and AR (standardized beta = 0.890, P < 0.001) from day 1 to 6 and PGRMC1 and LHB (standardized beta = 0.806, P < 0.001) from day 1 to 10 were found in poor responders. PGRMC1 and PGRMC2 were positively correlated on days 6 and 10 in normal responders.ConclusionsPGRMC1 and PGRMC2 mRNA are significantly decreased during ovarian stimulation, with some potential differences between normal and poor responders. 相似文献