CyclinL2在化疗药物杀伤胃癌细胞的增敏作用

目的：探讨cyclinL2基因在化疗药物顺铂（DI）P）、氟尿嘧啶（5-Fu）和多西紫杉醇（Doc）诱导下的胃癌细胞凋亡中的作用。方法选用胃腺癌BCG-823细胞，MTT法检测DDP、5-Fu和Doe不同药物浓度对胃癌细胞生长的抑制作用，以及CyclinL2转染、反义cyclinL2转染后对该细胞生长的抑制作用的影响，同时利用流式细胞术定量检测细胞凋亡。结果不同浓度的化疗药物对该胃癌细胞生长均有明显的抑制作用，并有浓度的依赖性。细胞凋亡率和cydinL2基因表达率成正相关。结论CydinL2基因在化疗药物诱导胃癌细胞凋亡中起重要作用。     

Perceived professional support and the use of blocking behaviours by hospice nurses

A prospective study of the impact of training 41 hospice nurses in assessment skills was used to test hypotheses that blocking behaviours would be used more when patients disclosed feelings and used less when nurses perceived that they had satisfactory professional support Each nurse was asked to assess a patient's current problems before and after feedback training and 8 months later Audiotape recordings of these interviews were rated by trained raters They determined the frequency of nurses' responses which had the function of blocking patient disclosure and the emotional level of patient disclosure Before each patient assessment each nurse was interviewed and questionnaires administered to measure her perceptions of the support she received Blocking behaviours were most evident when patients disclosed their feelings (Kendalls r=0 36, P < 0 001) In interviews containing most patient disclosure of feeling, blocking was significantly less (r= - 0 24, P < 0 5) when the nurse felt that practical help would be available if needed and when the nurse felt that her direct supervisor was concerned about the nurse's own welfare (r= -0 37, P < 0 005)