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211.
Human endothelial cells synthesize protein S 总被引:6,自引:0,他引:6
Human umbilical vein endothelial cells were analyzed for the presence of prothrombin, factor VII, protein C, and protein S in culture supernatants and cell extracts using specific radioimmunoassays. Only protein S was detected. Conditioned medium from 24-hour cultures and cell lysates contained 21.7 ng/mL and 88.8 ng/10(7) cells of protein S, respectively. Intrinsic labeling and immunoprecipitation indicated that protein S was synthesized and secreted as a 75,000 molecular weight protein. Vitamin K, phorbol myristate acetate, and thrombin increased the production or specific activity (determined from activity/antigen ratios of 0.99 to 1.07, 0.93 to 1.04, and 0.90 to 1.04, respectively) of protein S. While untreated cells secreted a partially active protein S (activity/antigen = 0.40), warfarin greatly decreased the specific activity (less than 0.10) of this molecule, suggesting that endothelial cells contain the enzymes required for the carboxylation of selected glutamic acid residues. The production of protein S by these cells supports the hypothesis that cofactor production and expression by the endothelial cells may play a significant regulatory role in the initiation, propagation, and suppression of hemostasis and thrombosis. 相似文献
212.
A 42 year-old male former semi-professional soccer player sustained a right lower extremity popliteal contusion during a soccer
game. He was clinically diagnosed with a possible traumatic deep vein thrombosis (DVT), and sent for confirmatory tests. A
duplex doppler ultrasound was positive for DVT, and the patient was admitted to hospital for anticoagulation (unfractionated
heparin, warfarin). Upon discharge from hospital the patient continued oral warfarin anticoagulation (six months), and the
use of compression stockings (nine months). He followed up with his family doctor at regular intervals for serial coagulation
measurements, and ultrasound examinations. The patient's only identified major thrombotic risk factor was the traumatic injury.
One year after the initial deep vein thrombosis (DVT) the patient returned to contact sport, however he continued to have
intermittent symptoms of right lower leg pain and right knee effusion. 相似文献
213.
Background
Although a grossly disproportionate burden of disease from HIV/AIDS, TB and malaria remains in the Global South, these infectious diseases have finally risen to the top of the international agenda in recent years. Ideal strategies for combating these diseases must balance the advantages and disadvantages of 'vertical' disease control programs and 'horizontal' capacity-building approaches. 相似文献214.
Mutations of factor VIII cleavage sites in hemophilia A 总被引:10,自引:1,他引:9
Hemophilia A is caused by a defect in coagulation factor VIII, a protein that undergoes extensive proteolysis during its activation and inactivation. To determine whether some cases of hemophilia are caused by mutations in important cleavage sites, we screened patient DNA samples for mutations in these sites by a two-step process. Regions of interest were amplified from genomic DNA by repeated rounds of primer- directed DNA synthesis. The amplified DNAs were then screened for mutations by discriminant hybridization using oligonucleotide probes. Two cleavage site mutations were found in a survey of 215 patients. A nonsense mutation in the activated protein C cleavage site at amino acid 336 was discovered in a patient with severe hemophilia. In another severely affected patient, a mis-sense mutation results in a substitution of cysteine for arginine in the thrombin activation site at amino acid 1689. This defect is associated with no detectable factor VIII activity, but with normal levels of factor VIII antigen. The severe hemophilia in this patient was sporadic; analysis of the mother suggested that the mutation originated in her gametes or during her embryogenesis. The results demonstrate that this approach can be used to identify factor VIII gene mutations in regions of the molecule known to be important for function. 相似文献
215.
216.
Magnetic resonance (MR) imaging of the patient with acute cervical injury is important because of the potential prognostic significance of the appearance of the spinal cord at the time of injury. However, cervical traction may involve equipment incompatible with the magnetic environment, and transferring the patient to the imaging table may make it difficult to maintain traction. The authors describe a simple, inexpensive, and reliable method for providing cervical traction within the magnet room. 相似文献
217.
Background
There is little experience with carefully developed interventions in the HIV/STI prevention field aimed at adult heterosexual target groups in the Netherlands. The ability to apply intervention development protocols, like Intervention Mapping, in daily practice outside of academia, is a matter of concern. An urgent need also exists for interventions aimed at the prevention of STI in migrant populations in the Netherlands. This article describes the theory and evidence based development of HIV/STI prevention interventions by the Municipal Public Health Service Rotterdam Area (MPHS), the Netherlands, for heterosexual migrant men with Surinamese, Dutch-Caribbean, Cape Verdean, Turkish and Moroccan backgrounds. 相似文献218.
219.
Andrzej B Pop?awski Micha? Jankowski Stephen W Erickson Teresita D��az de St?hl E Christopher Partridge Chiquito Crasto Jingyu Guo John Gibson Uwe Menzel Carl EG Bruder Aneta Kaczmarczyk Magdalena Benetkiewicz Robin Andersson Johanna Sandgren Barbara Zegarska Dariusz Ba?a Ewa ?rutek David B Allison Arkadiusz Piotrowski Wojciech Zegarski Jan P Dumanski 《European journal of human genetics : EJHG》2010,18(5):560-568
220.