Comparative analysis of secretion of S100A4 metastatic marker by immune and tumor cells

S100A4 protein is present in low concentrations (2.1–15.7 ng/10⁶ cells) in lymphocyte and neutrophil culture medium. Addition of stimulants to the cells did not lead to an appreciable increase in the content of this protein. The initial content of S100A4 is significantly higher (92–447 ng/10⁶ cells) in culture media of highly metastatic KSML-100 adenocarcinoma and M3 and B16 melanoma cells. The release of S100A4 by these cells significantly increased after addition of lymphocytes and Tag7/Hsp70 cytotoxic complex. Repeated injection of antibodies to S100A4 to mice with transplanted M3 melanoma inhibited tumor growth. __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 145, No. 1, pp. 85–87, January, 2008     

人工肝治疗重症妊娠期急性脂肪肝的临床疗效分析

目的:分析人工肝治疗重症妊娠期急性脂肪肝(AFLP)的临床疗效.方法:收集重庆医科大学附属第一医院产科2002年1月至2007年8月住院并行人工肝治疗的15例重症AFLP患者的临床资料.观察患者的症状、体征、人工肝治疗前后血常规、肝肾功能、电解质、凝血象等指标.判断病情变化.结果:①人工肝治疗后肝肾功能、凝血功能主要指标较治疗前有明显改善(P<0.05);②15例患者中,3例患者经过1次人工肝治疗后,症状及各项实验室指标逐渐好转而痊愈.其余12例经过2次甚至3次人工肝治疗,其中7例痊愈,5例死亡.人工肝治疗过程中患者不良反应轻微,无1例因不良反应而终止治疗;③共17例胎儿出生,其中死胎5例(单胎1例、双胎2例),另12例出生时,新生儿Apgar评分>7分7例,预后良好;Apgar评分<7分5例,新生儿死亡2例,经治疗痊愈3例.结论:人工肝支持治疗是重症AFLP综合治疗中一项重要的治疗手段,且应尽早使用以阻断病情进展.     

Analysis of Patients with Esophageal Varices Surviving More Than Three Years after Endoscopic Injection Sclerotherapy

Of the 205 patients treated by endoscopic injection sclerotherapy in the past 8 years and 4 months, 70 patients (34.1%) have survived more than 3 years. There were more Child's class A patients (p < 0.05) and fewer Child's C patients (p < 0.01) in this group as compared to 51 patients who died within 3 years. In addition, complications due to hepatoma were significantly lower (p < 0.01) in this group. The long-term cumulative survival rates of those who had already survived over 3 years were 82% at the 5-year and 78% at the 7-year follow-up. There was no significant difference among 3 groups classified by severity of liver damage or timing of the therapy. Rebleeding was noted in 13 patients (18.3%) and the cumulative bleeding rates were 9% at the 1-year, 14% at the 3-year, 18% at the 5-year and 21% at the 7-year follow-up. In 12 of these patients hemostasis was obtained by the second sclerotherapy. There was no significant difference in the long-term prognosis between patients who experienced repeat bleeding, and those who did not. Endoscopic findings in patients with rebleeding were characteristic in that the red color sign remained pronounced despite the fact that the varices had shrunk from F₂ or larger to F₁ in 6 patients. Bleeding occurred from the gastric varices in 4 patients. One of them died due to gastric bleeding, but 3 were operated on after sclerotherapy. For improving prognosis, it is important to carefully observe the clinical course and to perform additional aggressive treatments for complete obliteration of varices.     

Two linked blood pressure quantitative trait loci on chromosome 10 defined by dahl rat congenic strains

Aquantitative trait locus (QTL) for blood pressure was previously detected on rat chromosome 10 (RNO10) by linkage analysis and confirmed by the construction of congenic strains that encompass large regions of RNO10. In the present study, the rat RNO10 blood pressure QTL was dissected by the further construction of congenic substrains. The original congenic region was shown to contain 2 blood pressure QTLs (QTL 1 and QTL 2) approximately 24 cM apart. These were localized to a <2.6-cM region between markers D10Rat27 and D10Rat24 for QTL 1 and to a <3.2-cM region between D10Rat12 and D10Mco70 for QTL 2. Comparative mapping suggests that the rat RNO10 QTL 2 could be localized very close to a blood pressure QTL described by sib-pair analysis on human chromosome 17, but this is not definitively established because of multiple and complex chromosomal rearrangements between rodents and humans.