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991.
Florien van Heest Ilora Finlay Renée Otter Betty Meyboom-de Jong 《The British journal of general practice》2007,57(539):494-496
This study describes a novel type of support for GPs caring for patients dying at home: the establishment and evaluation of a telephone advisory service for GPs, run by GPs with a special interest in palliative care (GPwSIs) in the Netherlands 2000-2003. A growing number of GPs called for advice, 10% during out of hours. Prognosis of the patients was generally short (days to weeks in 70% of cases). Most advice sought by GPs concerned symptom management and on evaluation, 85% of the GPs followed the advice. 相似文献
992.
Eimeria tenella microneme protein EtMIC3: identification, localisation and role in host cell infection 总被引:1,自引:0,他引:1
Labbé M de Venevelles P Girard-Misguich F Bourdieu C Guillaume A Péry P 《Molecular and biochemical parasitology》2005,140(1):43-53
The gene coding for Eimeria tenella protein EtMIC3 was cloned by screening a sporozoite cDNA library with two independent monoclonal antibodies raised against the oocyst stage. The deduced sequence of EtMIC3 is 988 amino acids long. The protein presents seven repeats in tandem, with four highly conserved internal repeats and three more divergent external repeats. Each repeat is characterised by a tyrosine kinase phosphorylation site, WRCY, and a reminiscent motif of the thrombospondin1 (TSP1)-type I domain, CXXXCG. The protein EtMIC3 is localised at the apex of free parasite stages. It is not detected in the early intracellular parasite stage but is synthesised in mature schizonts. Secretion of the protein is induced when sporozoites are incubated in complete medium at 41 degrees C. Strangely enough, the two independent mAb that allow cloning of EtMIC3 interfere with parasitic growth in different ways. One is able to inhibit parasite invasion whereas the other inhibits development. Expression and localisation of the protein EtMIC3 are consistent with a protein involved in the invasion process as is expected for a microneme protein. 相似文献
993.
Summary We have characterized the ribosomal proteins from Spinacia chloroplasts using two-dimensional gel electrophoresis. The 30S and 50S subunits contain 23–25 and 36 ribosomal proteins, respectively. In contrast to prokaryotic ribosomes, chloroplast ribosomes contain at least one (and possibly two) phosphorylated ribosomal proteins. Isolated chloroplasts synthesize in the presence of (35S) labeled methionine and cysteine at least seven 30S and thirteen 50S ribosomal proteins which are assembled into (pre)ribosomes. This suggests that about one third of the chloroplast ribosomal proteins is encoded by the chloroplast DNA itself. The identity of several labeled proteins in the two-dimensional gel electrophoretic patterns which did not comigrate with stained chloroplast ribosomal proteins is discussed.Abbreviations CBB
Coomassie Brilliant Blue
- CHI
cycloheximide
- cp
chloroplast
- DTT
dithiotreitol
- EDTA
ethylene diamine tetraacetate
- EGTA
ethylene glycol-bis (-amino ethyl ether) N,N-tetraacetic acid
- kD
kilodalton
- LHCP
light harvesting chlorophyll a/b protein
- PMSF
phenyl methyl sulfonyl fluoride
- RuBPCase
ribulose-1,5-bisphosphate carboxylase
- SDS
sodiumdodecylsulphate 相似文献
994.
The authors found tardive dyskinesia (TD) in 23.5% of 200 hospitalized schizophrenic patients, most of whom had received neuroleptic treatment for at least two years. The frequency and severity of TD increased with age, and the more advanced the age at which the patient started taking neuroleptics, the more likely it was that TD would develop. Severe TD was more common in men than in women. Prolonged treatment with neuroleptics or the use of antiparkinsonism drugs increased the risk of TD. 相似文献
995.
Hera Der-Sarkissian Catherine Bonaïti-Pellié Marie-Louise Briard-Guillemot Jean-Michel Zucker 《Cancer Genetics and Cytogenetics》1982,7(1):73-77
Spontaneous and mitomycin C(MMC)-induced sisyer chromatid exchanges were studied in 11 patients with retinoblastoma and 7 normal controls. Spontaneous rates were similar in patients and in controls. The MMC-induced rate was found to be significantly higher in bilaterally affected patients than in controls. It is suggested that this increase may be due to a DNA repair deficiency. However, it is not possible to clarify wether this abnormality is associated with the retinoblastoma gene or with another factor acting on the degree of expressivity of the disease in gene carriers. 相似文献
996.
The energetics of middle-distance running 总被引:3,自引:0,他引:3
J. R. Lacour S. Padilla-Magunacelaya J. C. Barthélémy D. Dormois 《European journal of applied physiology》1990,60(1):38-43
Summary In order to assess the relative contribution of aerobic processes to running velocity (v), 27 male athletes were selected on the basis of their middle-distance performances over 800, 1500, 3000 or 5000 m, during the 1987 track season. To be selected for study, the average running velocity
corresponding to their performances had to be superior to 90% of the best French
of the season. Maximum O2 consumption
and energy cost of running (C) had been measured within the 2 months preceding the track season, which, together with oxygen consumption at rest
allowed us to calculate the maximalv that could be sustained under aerobic conditions:
. The treadmill runningv corresponding to a blood lactate of 4 mmol·–1 (v
la4), was also calculated. In the whole group, C was significantly related to height (r=–0.43;P<0.03). Neither C nor
(with, in this case, the exception of the 3000 m athletes) were correlated to
. On the other hand,v
a max was significantly correlated to
over distances longer than 800 m. These
were also correlated tov
la4. Howeverv
la4 occurred at 87.5% SD 3.3% ofv
a max, this relationship was interpreted as being an expression of the correlation betweenv
a max and
. Calculation ofv
a max provided a useful means of analysing the performances. At the level of achievement studied,
sustained over 3000 m corresponded tov
a max. The shape of the relationship ofv/v
a max as a function of the duration of the event raised the question of a possible change in C as a function of v during middle-distance running competitions. 相似文献
997.
Jennifer A. Ruskey Lior Greenbaum Léanne Roncière Armaghan Alam Dan Spiegelman Christopher Liong Oren A. Levy Cheryl Waters Stanley Fahn Karen S. Marder Wendy Chung Gilad Yahalom Simon Israeli-Korn Vered Livneh Tsvia Fay-Karmon Roy N. Alcalay Sharon Hassin-Baer Ziv Gan-Or 《European journal of medical genetics》2019,62(1):65-69
Background
Variants in GBA are the most common genetic risk factor for Parkinson's disease (PD), and are especially prevalent in the Ashkenazi Jewish (AJ) population. However, most studies on GBA in AJ genotype only seven selected Gaucher-associated pathogenic variants rather than sequencing the whole gene, which may leave carriers of PD-associated GBA variants undiscovered.Methods
GBA was fully sequenced using molecular inversion probes (MIPs) and Sanger sequencing in 735 AJ PD patients and 662 AJ controls, from Israel and New York. Additional AJ control data (n?=?3044) from the Inflammatory Bowel Disease Exome Portal was used.Results
Full GBA sequencing increased the number of variants discovered by 17.4%, compared to targeted genotyping. An additional 17 PD patients were identified with GBA-associated PD. The p.E326K variant was found in 1.6% of AJ PD patients, making it the second most common PD-associated GBA variant in AJ. GBA variants were found in 18% of PD patients and 7.5% of controls (OR?=?2.7, 95%CI?=?1.9–3.8, p?<?0.0001).Conclusion
Without full sequencing of GBA, or at minimum including p.E326K in the genotyping panel, a significant proportion of variant carriers go undiscovered and may be incorrectly assigned as non-carriers in studies or clinical trials. 相似文献998.
Lymphocyte cultures from homozygotes for the beta-thalassaemia gene were found to contain chromosomal gaps, breaks, and rearrangements more often than those from controls. Culture time seemed to have a determinant effect on the results. The possible influence of folic acid deficiency is discussed. 相似文献
999.
Emoto M Zerrahn J Miyamoto M Pérarnau B Kaufmann SH 《European journal of immunology》2000,30(8):2300-2311
We describe a novel CD8(+)NKT cell population expressing TCRalpha /beta or TCRgamma /delta. These CD8(+)NKT cells were prominent in the liver, and except for the thymus, virtually absent in other lymphoid organs. CD8(+)NKT cells expressed activation markers and comprised a high proportion of Ly49(+) cells. The development of the majority of CD8(+)NKT cells expressing TCRalpha /beta, but not TCRgamma /delta, depended on classical MHC class I. No CD8(+)NKT cells were detectable in young athymic mice, whereas the cells expressing TCRgamma /delta, but not TCRalpha /beta, appeared randomly in aged athymic mice. CD8(+)NK1(+) TCRalpha /beta cells showed polyclonal TCRVbeta usage and were virtually devoid of TCRValpha14. CD8(+)NK1(+) TCRgamma /delta cells predominantly expressed TCRVgamma1, 2 and 4, and Vdelta4, 5, 6 and 7. CD8(+)NKT cells, in particular those expressing TCRgamma /delta, were a major population in early life. IFN-gamma, but not IL-4, was induced in CD8(+)NKT cells by in vitro stimulation, independent of the TCRalpha /beta or TCRgamma /delta lineage. Hence, these cells represent a unique, though heterogeneous T cell population that shares markers with, but is distinct from, both conventional NKT cells and conventional CD8(+) T cells, and that may play a role in immune regulation. 相似文献
1000.
Stéphane Genet Raymond T. Kado 《Pflügers Archiv : European journal of physiology》1997,434(5):559-567
The contribution of the Na/K ATPase (pump) current to the polarization of the Purkinje cell has been studied using slices
of the rat cerebellum by blocking the pump with dihydro-ouabain (DHO) while recording the membrane potential with microelectrodes
in the somata. From our recordings, it appeared that blocking the pump depolarized the Purkinje cells more rapidly than might
be expected from shifts in Na+ and K+ concentrations, suggesting the removal of a hyperpolarizing current. Application of DHO, in the presence of tetrodotoxin (TTX),
led to calcium spike firing and plateau-like discharges suggesting activation of voltage-dependent calcium channels in the
dendrites. Adding 2 mM Co2+ to the medium did not prevent the depolarizations. Removing calcium from the bathing medium containing 2 mM Co2+ blocked the spiking activity but DHO application still produced a depolarization. Experiments to measure the current inhibited
by DHO indicated that the Na/K pump supplies a constant current of 240 pA. Substitution of the sodium with choline produced
a hyperpolarization, during which DHO had no effect on the membrane potential. Substitution of the sodium with lithium produced
only a slowly developing depolarization. It is concluded that in the cerebellar Purkinje cell, a continuous sodium ion influx
activates the pumps which produce a current that directly contributes to the membrane polarization. Possible pathways for
this sodium influx are discussed.
Received: 3 April 1997 / Received after revision and accepted: 12 May 1997 相似文献