家兔早期酒精性股骨头坏死的生物化学改变

为探讨长期过量饮酒时家兔血清生物化学改变及其在股骨头缺血性坏死（ANFH）中所起的作用。取健康家兔80只，随机分为实验组和对照组，每组40只。采用灌胃法，实验组给予烈性酒（（乙醇）＝45％）10ml／（kg·d），对照组给予100g／L的葡萄糖溶液10ml／（kg·d）。2组动物在实验第1、2、3、6个月时分批处死。结果显示：实验组动物血清LPO、GGT、ALT、AST、TG和CH升高，SOD降低；病理组织学检查见脂肪肝和股骨头缺血性坏死呈早期改变。提示：生化代射异常变化与酒精性ANFH的发生发展密切相关。     

间期细胞核中abl和bcr基因的三维空间分布在bcr／abl融合基因形成中的作用

目的 从生物体视学角度分析bcr/abl融合基因的形成机制。方法 应用荧光原位杂交和激光共聚焦扫描显微镜技术，观察γ－射线对IM9细胞中的bcr和abl基因在间期核内三维空间分布的影响。结果 abl和bcr基因在间期核内有其特定的分布区域，且该分布区域在细胞周期中呈规律性的动态变化。放射性照射会使abl和bcr基因之间的距离缩短。结论 abl和bcr基因在间期细胞核内的易接近性是bcr/abl融合基因形成的因素之一。     

肢端皮肤角化过度增生症的手术治疗

目的介绍肢端皮肤角化过度增生症１０例的治疗体会，其中掌跖角化病９例，疣状表皮结构不良１例。方法采用病变皮肤切除皮肤移植术治疗，其中两例有深部组织外露和放射性溃疡选用皮瓣修复，其余行皮片移植。结果１０例均获满意效果，追踪观察８年以上，无复发，能保持接近正常的功能活动。结论手部以皮片效果最佳，足底应用皮瓣修复，耐磨性较皮片为好，但因皮瓣缺乏纤维隔结构，走路时皮瓣与深部组织间产生相对滑动，也容易造成破溃，必须注意妥为保护     

我国散发性戊型肝炎病毒细胞培养分离株的鉴定

我国散发性戊型肝炎病毒细胞培养分离株的鉴定１００８５０北京军事医学科学院微生物流行病研究所苑锡同，李晓萸，黄如统，李德荣关键词肝炎病毒，戊型；病毒细胞培养；病毒鉴定；聚合酶链反应；核酸杂交中国图书资料分类号Ｑ９３９．４我国戊型肝炎存在着流行和散发两种...     

M129V polymorphism in the prion protein gene is not associated with mesial temporal lobe epilepsy in a Han Chinese population

Background and purpose:  Prion protein (PrP) predominantly localized at synapses can modulate neuronal excitability. The prion protein gene ( PRNP ) has been considered one of the candidate genes that play a role in seizure susceptibility. A recent study demonstrated that the 129V allele in the PRNP gene was associated with susceptibility to temporal lobe epilepsy (TLE) in female patients in an Italian population. We screened variations in the open-reading frame (ORF) of the PRNP gene and also replicated the association of the M129V polymorphism with TLE in a Han Chinese population.
Methods:  The M129V polymorphism was genotyped in 320 MTLE patients and 558 non-epilepsy controls. All subjects were Han Chinese.
Results:  No novel polymorphism in the ORF of the PRNP gene was detected. Differences in the genotype distributions and allele frequencies of this polymorphism between cases and controls were insignificant ( P  =   0.24). Further analysis with stratification of the results by gender or age and analysis of clinical features in relation to M129V genotypes also yielded negative findings.
Conclusions:  The present study provides evidence that the M129V polymorphism in the PRNP gene is not associated with MTLE in a Han Chinese population.     

痔上黏膜环切钉合术与Milligan—Morgan术治疗Ⅲ、Ⅳ度内痔2年疗效比较

目的比较痔上黏膜环切钉合术（PPH）与Milligan—Morgan术（MMH）治疗Ⅲ、Ⅳ度内痔的远期疗效。方法按照完全随机分组原则将100例Ⅲ、Ⅳ度内痔患者分别采用PPH（PPH组，42例）和MMH（MMH组，58例）手术，观察并比较两组患者术后的远期疗效差异。结果PPH组与MMH组术后2年比较，在出现肛门溢液（2．38％比20．69％，P=0．007）、皮赘（9．52％比25．86％，P=0．040）和细便（2．38％比18．97％，P=0．027）方面PPH组术后少见，总的并发症发生率（9．52％比25．86％，P=0．040）和总排便功能异常（9．52％比29．31％，P=0．017）方面PPH组术后也少见，两组差异有统计学意义。在痔脱垂复发（14．29％比10．34％，P=0．549）、症状改善满意度（92．86％比87．93％，P=0．636）和总的症状复发（19．05％比25．86％，P=0．424）方面两组差异无统计学意义。结论PPH与Milligan-Morgan术治疗Ⅲ、Ⅳ度内痔的远期疗效无显著差异，但PPH安全、并发症少、对排便功能影响小。     

Improved gas chromatographic quantitation of breath hydrogen by normalization to respiratory carbon dioxide.

A gas-solid chromatographic system using tandem silica gel and molecular sieve columns is described for the measurement of hydrogen, carbon dioxide, oxygen, and nitrogen in samples of respiratory gases. This system has a detection limit of 2 ppm of hydrogen in a 1 ml sample and can measure 120 ppm H2 and 5% CO2 with relative standard deviations of 1.3% and 1.7%, respectively. Improved sample storage and withdrawal techniques are described that give reproducible values for up to 6 weeks after collection. Finally we show that normalization of breath hydrogen values to an alveolar concentration, using the observed carbon dioxide concentrations, substantially reduces the range and variance of apparent H2 concentrations in human subjects. Normalization eliminates the need for rebreathing or end-expiratory collection techniques and substantially increases the reliability and clinical utility of hydrogen breath measurements in noninvasive tests of carbohydrate malabsorption.     

A Clinical and Experimental Study on the Protective Effects of Jiangzhi Tongmai Fang on Endothelial Injury

31 cases of atherosclerosis (AS) were treated with Jiang Zhi Tong Mai Fang ([symbol: see text], formula of JZTMF), and its effect was compared with 30 cases treated with lovastatin in the control group. Clinically, the JZTMF formula showed an effect of regulating blood lipids, and therefore it was antiatherosclerotic. The mechanism is, probably, restoration of the function of endothelial cells (EC) by increasing the synthesis of 6-keto-PGF1 alpha and decreasing the release of endothelin (ET) as evidenced in the experimental study.     

SPONTANEOUS DISRUPTION OF BOVINE PERICARDIAL PROSTHESIS CLINICAL MANAGEMENT AND HISTOPATHOLOGIC CHANGES

Valve failure is rate after using glutaraldehyde stabilized bovine pericardial bioprosthesis in the mitral position.i This article reports a case of spon- taneous disruption of bovine pericardial xenograft after mitral valve replacement five years after opera- tion.