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12.
Flores-Figueroa E Varma S Montgomery K Greenberg PL Gratzinger D 《Laboratory investigation; a journal of technical methods and pathology》2012,92(9):1330-1341
Mesenchymal stromal cells (MSCs) support hematopoiesis and are cytogenetically and functionally abnormal in myelodysplastic syndrome (MDS), implying a possible pathophysiologic role in MDS and potential utility as a diagnostic or risk-stratifying tool. We have analyzed putative MSC markers and their relationship to CD34+ hematopoietic stem/progenitor cells (HSPCs) within intact human bone marrow in paraffin-embedded bone marrow core biopsies of benign, MDS and leukemic (AML) marrows using tissue microarrays to facilitate scanning, image analysis and quantitation. We found that CD271+, ALP+ MSCs formed an extensive branching perivascular, periosteal and parenchymal network. Nestin was brightly positive in capillary/arteriolar endothelium and occasional subendothelial cells, whereas CD146 was most brightly expressed in SMA+ vascular smooth muscle/pericytes. CD271+ MSCs were distinct by double immunofluorescence from CD163+ macrophages and were in close contact with but distinct from brightly nestin+ and from brightly CD146+ vascular elements. Double immunofluorescence revealed an intimate spatial relationship between CD34+ HSPCs and CD271+ MSCs; remarkably, 86% of CD34+ HSPCs were in direct contact with CD271+ MSCs across benign, MDS and AML marrows, predominantly in a perivascular distribution. Expression of the intercrine chemokine CXCL12 was strong in the vasculature in both benign and neoplastic marrow, but was also present in extravascular parenchymal cells, particularly in MDS specimens. We identified these parenchymal cells as MSCs by ALP/CXCL12 and CD271/CXCL12 double immunofluorescence. The area covered by CXCL12+ ALP+ MSCs was significantly greater in MDS compared with benign and AML marrow (P=0.021, Kruskal-Wallis test). The preservation of direct CD271+ MSC/CD34+ HSPC contact across benign and neoplastic marrow suggests a physiologically important role for the CD271+ MSC/CD34+ HSPC relationship and possible abnormal exposure of CD34+ HSPCs to increased MSC CXCL12 expression in MDS. 相似文献
13.
Kristyna Hrncirova Martina Lengerova Iva Kocmanova Zdenek Racil Pavlina Volfova Dita Palousova Mojmir Moulis Barbora Weinbergerova Jana Winterova Martina Toskova Sarka Pospisilova Jiri Mayer 《Journal of clinical microbiology》2010,48(9):3392-3394
We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.Invasive infections caused by mucormycetes started to occur more frequently in the last decade and are connected with rapid progression and high mortality rates. Early diagnostics and targeted treatment are crucial. Most mucormycosis cases (over 90%) are caused by Rhizopus spp., followed by Mucor spp., Lichtheimia spp., Rhizomucor pusillus, and, rarely, some other species (2, 9, 11, 16).Definitive diagnosis of mucormycosis is usually made after histopathological proof of mucormycete-like hyphae in involved tissue; the causative agent can be determined only by culture (13). So far, no serological test is available and radiological methods are nonspecific.Molecular detection of mucormycetes is complicated by several factors, and we still do not have any standard protocol. Few methods for the detection of mucormycetes have been published, and only some have been evaluated using clinical samples (1, 5, 10, 14, 15, 17) or samples from animal models (6, 7).The aim of this study was to develop a rapid and sensitive technique for the detection and identification of clinically important mucormycetes. We adopted primers from a qualitative method previously published by Bialek et al. (1) that is specific for members of the order Mucorales targeting 18S ribosomal DNA (rDNA). We modified it to seminested real-time PCR with EvaGreen dye, followed by species distinction by high-resolution melt (HRM) analysis. HRM analysis uses amplification of DNA in the presence of intercalation dye. Fluorescence is measured during a controlled melting of PCR product that results in a melt curve that depends mainly on GC content, length, and sequence of the PCR product. This simple method can be used for genotyping or mutation scanning without the need for time-consuming sequencing (4, 12).DNA was isolated from 50 μl of fungal culture (inoculum was prepared by covering sporulating colonies with approximately 2 ml of sterile 0.85% saline) or a piece of fresh tissue (2 by 1 mm) using the ZR fungal/bacterial DNA kit (Zymo Research). Tissue samples were incubated in lysis buffer overnight, and cultures were immediately processed according to the manufacturer''s protocol. Disruption was extended to 15 min (Disruptor Genie; Scientific Industries). DNA from formalin-fixed, paraffin wax-embedded (FFPE) tissue samples was isolated from 2 or 3 scrolls (5 to 10 μm each) of paraffin block using a DNeasy blood and tissue kit (Qiagen). Paraffin was dissolved in 1 ml of xylene, and then the tissue was washed two times using 1 ml of 96% ethanol and incubated in 180 μl of ATL buffer (Qiagen) and 20 μl of proteinase K (600 mAU/ml solution, where one mAU represents the activity of proteinase K that releases folin-positive amino acids and peptides corresponding to 1 μmol of tyrosine per min) at 55°C overnight and then at 90°C for 1 h. The next steps were done in accordance with the manufacturer''s protocol. DNA isolation from clinical samples was done in a biological safety cabinet. An aliquot of sterile water was processed with each set of samples as a control of potential contamination during the isolation process.Five microliters of DNA was amplified in 25 μl of amplification mixture that contained a 0.2 μM concentration each of primers ZM1 and ZM2 (1), 120 μM deoxynucleoside triphosphates (dNTPs; Roche, Germany), 2.5 mM MgCl2, 1× GeneAmp PCR Gold buffer, and 1.5 U AmpliTaq Gold DNA polymerase (Applied Biosystems). The cycling conditions were 10 min at 95°C, 16 cycles of 30 s at 94°C, 30 s at 50°C, and 60 s at 72°C, and 7 min at 72°C. One microliter of PCR product from the external round was then amplified in duplicate using Rotorgene 6000 (Corbett Research, Australia). Twenty-five microliters of the amplification mixture contained a 0.4 μM concentration each of primers ZM1 and ZM3 (1), 12.5 μl of SensiMix HRM, and 1 μl of EvaGreen (both from a SensiMix HRM kit; Quantace, United Kingdom). The cycling conditions were 10 min at 95°C, followed by 40 cycles of 15 s at 95°C, 20 s at 60°C, and 30 s at 72°C (acquired on the green channel), followed by HRM analysis (ramp from 74°C to 79.5°C, rising by 0.1°C each cycle, acquired on the HRM channel). Rotorgene 6000 series software (version 1.7) was used for analysis of the results. All positive results were confirmed by sequencing of the PCR product. DNA was purified using a QIAquick PCR purification kit (Qiagen, Germany) and sequenced using a BigDye Terminator v1.1 cycle sequencing kit (Applied Biosystems) on an ABI Prism 310 genetic analyzer (Applied Biosystems). Sequences were analyzed using the BLAST alignment program of the GenBank database.We used DNA extracted from five mucormycete cultures diluted in Tris-EDTA (TE) buffer as positive controls in every run. A DNA isolation control (sterile water processed with clinical samples) and a negative control of PCR (sterile water) were added to each run as well.In this study, we tested 31 fungal isolates, comprising 10 mucormycete isolates and 21 isolates from other filamentous fungal groups (Department of Clinical Microbiology, University Hospital Brno and Czech Collection of Microorganisms, Czech Republic). All mucormycete isolates were correctly identified. The melting temperatures (Tm) for each species were as follows: for Rhizopus microsporus, 76.46°C; for Rhizopus oryzae, 76.59°C; for Mucor racemosus, 76.78°C; for Mucor circinelloides, 76.98°C; for Rhizomucor pusillus, 77.87°C; and for Lichtheimia corymbifera, 78.56°C. Representative HRM curves for six different mucormycetes are shown in Fig. Fig.1.1. All HRM analysis results were confirmed by sequencing. None of the nonmucormycete fungi were positively tested. The results are summarized in Table Table11.Open in a separate windowFIG. 1.Representative result of high-resolution melt (HRM) analysis. Shown are HRM curves for six mucormycete isolates (black curves) and one negative and one positive tissue sample (gray curves).
Open in a separate windowaCCM, Czech Collection of Microorganisms, Czech Republic; DCM, Department of Clinical Microbiology, University Hospital Brno, Czech Republic.We also tested 12 tissue samples, 7 (6 fresh and 1 FFPE) from patients with histopathologically or culture-proven mucormycosis and 5 (3 fresh and 2 FFPE) from patients without mucormycosis (obtained from hemato-oncological patients from University Hospital Brno, Czech Republic). All seven tissue samples from patients with proven mucormycosis were PCR positive, and in all cases, we were able to directly determine the mucormycete species: R. microsporus (n = 4), L. corymbifera (n = 2), and R. pusillus/miehei (these two species have 100% sequence homology in the target region and therefore cannot be distinguished; n = 1). All five tissue samples from patients without mucormycosis were negative. Results are summarized in Table Table2,2, and representative HRM analysis curves are shown in Fig. Fig.1.1. Amplification of fragmented DNA from FFPE samples can be problematic (8). In this study, we tested one FFPE tissue from a patient with proven mucormycosis, and the result was positive.
Open in a separate windowThe sensitivity of the method was assessed by amplification of dilutions (2 × 107 to 2 × 100 copies/5 μl) of plasmid DNA (external PCR products of R. pusillus and L. corymbifera cloned into the pCR2.1 vector; Invitrogen). Reproducible melt curves were obtained for concentrations up to 0.1 fg of plasmid DNA, the detection limit corresponding to the original qualitative method (1), in both species.To assess potential PCR inhibition, human albumin gene was detected by real-time PCR (3) in all tissue samples. No inhibition was observed.In conclusion, the HRM assay presented is very simple and enables rapid and accurate detection and identification of mucormycetes in tissue samples and culture isolates. It is able to distinguish the main clinically relevant mucormycetes and shows no cross-reactivity with nonmucormycete filamentous fungi. It is highly sensitive and specific and is suitable for routine clinical diagnostics. Its potential for use in diagnostics with other clinical materials, such as bronchoalveolar lavage fluid, sputum, etc., needs further study but is evident. 相似文献
TABLE 1.
List of fungal isolates used in this study and results of HRM analysisaOrganism | Accession no. or source | Result of zygomycete HRM analysis |
---|---|---|
Mucormycetes | ||
Rhizopus oryzae | Clinical isolate; DCM | Rhizopus oryzae |
CCM 8075 | Rhizopus oryzae | |
Rhizopus sp. | Clinical isolate; DCM | Rhizopus oryzae |
Rhizopus microsporus | Clinical isolate; DCM | Rhizopus microsporus |
Rhizomucor pusillus | CCM F-211 | Rhizomucor pusillus |
Mucor racemosus | CCM 8190 | Mucor racemosus |
Mucor circinelloides | Clinical isolate; DCM | Mucor circinelloides |
Lichtheimia corymbifera | CCM 8077 | Lichtheimia corymbifera |
Clinical isolate; DCM | Lichtheimia corymbifera | |
Clinical isolate; DCM | Lichtheimia corymbifera | |
Other filamentous fungi | ||
Fusarium oxysporum | Clinical isolate; DCM | Negative |
Clinical isolate; DCM | Negative | |
Fusarium proliferatum | Clinical isolate; DCM | Negative |
Fusarium solani | CCM 8014 | Negative |
Aspergillus fumigatus | Clinical isolate; DCM | Negative |
Clinical isolate; DCM | Negative | |
Aspergillus niger | Clinical isolate; DCM | Negative |
CCM 8155 | Negative | |
Aspergillus flavus | CCM 8363 | Negative |
CCM F-171 | Negative | |
Aspergillus terreus | CCM 8082 | Negative |
Aspergillus ustus | CCM F-414 | Negative |
Aspergillus nidulellus (nidulans) | CCM F-266 | Negative |
Aspergillus sydowii | Environment; DCM | Negative |
Scedosporium apiospermum | Clinical isolate; DCM | Negative |
Cladosporium cladosporioides | Environment; DCM | Negative |
Cladosporium cladosporioides f. sp. pisicola | CCM F-348 | Negative |
Penicillium commune | CCM F-327 | Negative |
Penicillium brevicompactum | CCM 8040 | Negative |
Environment; DCM | Negative | |
Penicillium chrysogenum | Environment; DCM | Negative |
TABLE 2.
List of tissue samples used in this study and results of HRM analysisPatient | Tissue sample | Histopathology result | Culture result | HRM analysis result |
---|---|---|---|---|
1 | Lung | Positive | Negative | Rhizopus microsporus |
2 | Lung (FFPE) | Positive | Negative | Rhizomucor pusillus/miehei |
3 | Oral cavity | Positive | Lichtheimia corymbifera | Lichtheimia corymbifera |
4 | Lung | Positive | Rhizopus microsporus | Rhizopus microsporus |
5 | Lung | Positive | Lichtheimia corymbifera | Lichtheimia corymbifera |
6 | Oral cavity 1 | Positive | Rhizopus microsporus | Rhizopus microsporus |
Oral cavity 2 | Positive | Rhizopus microsporus | Rhizopus microsporus | |
7 | Lung | Negative | Negative | Negative |
8 | Lung | Negative | Negative | Negative |
9 | Lung (FFPE) | Negative | Negative | Negative |
10 | Lung | Negative | Negative | Negative |
11 | Lung (FFPE) | Negative | Negative | Negative |
14.
Authors evaluated the prevalence of symptoms of the metabolic syndrome and insulin resistance in 25 patients with adrenal incidentalomas (10 men, 15 women) of the mean age 57.9+/-15 years. 15 patients had adrenal adenoma determined by CT or MR scan and 10 had unilateral or bilateral hyperplasia. The prevalence of obesity was 72%, arterial hypertension 60%, diabetes mellitus or impaired glucose tolerance 28%, hyperlipidemia 56% and hyperuricemia 20%, respectively, which is more frequent occurrence than that in normal human population. Patients with adrenal adenomas had mildly but significantly higher body mass index (BMI, p<0.05) and insulin resistance calculated as HOMA IR (p<0.05) and FIRI (p<0.05) and significantly higher values of serum ferritin (p<0.01). Plasma cortisol values were slightly but not significantly higher in the group with adrenal adenomas. Authors conclude that adrenal adenomas are probably more related to the metabolic syndrome than adrenal hyperplasia. 相似文献
15.
Emmanuel A. Ho Maryam Osooly Dita Strutt Dana Masin Youngjoo Yang Hong Yan Marcel Bally 《Journal of pharmaceutical sciences》2013,102(1):227-236
Polyethylene glycol (PEG) has been used widely in liposomal formulations as a strategy to inhibit opsonization by plasma proteins and to prolong liposome plasma circulation time. PEG can be incorporated onto the surface of liposomes either during the spontaneous self‐assembling process or inserted after vesicle formation. The advantages of employing the PEG postinsertion method include improved drug encapsulation efficiency and the ability to incorporate PEG conjugates for enhanced cell binding and uptake. In this study, we propose to evaluate a cationic lipid nanoparticle formulation containing two PEGylation steps: pre‐ and post‐siRNA insertion. Our results indicate that formulations consisting of the extra PEG post‐insertion step significantly increased siRNA circulation in the plasma by two‐folds in comparison with the formulations consisting of only the single PEGylation step. Moreover, this formulation was able to efficiently carry siRNA to the tumor site, increase siRNA stability and significantly downregulate luciferase mRNA expression by >50% when compared with the controls in an intraperitoneal and subcutaneous breast cancer tumor model. Overall, our cationic lipid nanoparticle formulation displayed enhanced plasma circulation, reduced liver accumulation, enhanced tumor targeting, and effective gene knockdown‐–demonstrating excellent utility for the delivery of siRNA. 相似文献
16.
Rynekrova J Kasparova D Adamkova V Fait T Hubacek JA 《American journal of reproductive immunology (New York, N.Y. : 1989)》2012,67(3):179-183
Citation Rynekrova J, Kasparova D, Adamkova V, Fait T, Hubacek JA. Analysis of the potential role of apolipoprotein E polymorphism in genetic predisposition to spontaneous abortion. Am J Reprod Immunol 2012; 67: 179–183 Problem Up to 20% of pregnancies end in the first trimester by spontaneous abortion, but a significant number remains unexplained. The aim of this study is to investigate the role of variants within the gene for apolipoprotein E (APOE) in the genetic determination of spontaneous abortions. Method of study We collected DNA from 410 tissue samples of spontaneous abortions, and APOE was genotyped by PCR–RFLP method. The frequencies were compared with a population sample of adults (N = 2606) and with a positive control (1060 women with at least two children). Results The frequencies of the APOE genotypes in abortions (APOE2E2 + E3E2 = 0.132; APOE3E3 = 0.661; APOE3E4 + E4E4 = 0.195; APOE4E2 = 0.012) did not significantly differ (P = 0.604) from the frequencies in analyzed adult population study (APOE2E2 + E3E2 = 0.132; APOE3E3 = 0.686; APOE3E4 + E4E4 = 0.169; APOE4E2 = 0.014) or from the positive control (APOE2E2 + E3E2 = 0.133; APOE3E3 = 0.691; APOE3E4 + E4E4 =0.166; APOE4E2 = 0.010; P = 0.592). Conclusion Our study suggests that APOE may not be associated with spontaneous abortions in Caucasians. 相似文献
17.
Schärer L Littlewood DT Waeschenbach A Yoshida W Vizoso DB 《Proceedings of the National Academy of Sciences of the United States of America》2011,108(4):1490-1495
Sperm are the most diverse of all animal cell types, and much of the diversity in sperm design is thought to reflect adaptations to the highly variable conditions under which sperm function and compete to achieve fertilization. Recent work has shown that these conditions often evolve rapidly as a consequence of multiple mating, suggesting a role for sexual selection and sexual conflict in the evolution of sperm design. However, very little of the striking diversity in sperm design is understood functionally, particularly in internally fertilizing organisms. We use phylogenetic comparative analyses covering 16 species of the hermaphroditic flatworm genus Macrostomum to show that a complex sperm design is associated with reciprocal mating and that this complexity is lost secondarily when hypodermic insemination--sperm injection through the epidermis--evolves. Specifically, the complex sperm design, which includes stiff lateral bristles, is likely a male persistence trait associated with sexual conflicts over the fate of received ejaculates and linked to female resistance traits, namely an intriguing postcopulatory sucking behavior and a thickened epithelium of the sperm-receiving organ. Our results suggest that the interactions between sperm donor, sperm, and sperm recipient can change drastically when hypodermic insemination evolves, involving convergent evolution of a needle-like copulatory organ, a simpler sperm design, and a simpler female genital morphology. Our study documents that a shift in the mating behavior may alter fundamentally the conditions under which sperm compete and thereby lead to a drastic change in sperm design. 相似文献
18.
Li Xinyi Sullivan Patrick Broz Dita Handanagic Senad 《Archives of sexual behavior》2022,51(5):2667-2678
Archives of Sexual Behavior - Persons who inject drugs (PWID) engaging in receptive syringe sharing with their sex partner (dual partnership) may have different behavior patterns than people who... 相似文献
19.
20.