羟甲基戊二酰辅酶A还原酶抑制剂的研究进展

目的：介绍ＨＭＧＣｏＡ 还原酶抑制剂类药的研究进展,为临床合理用药提供参考。方法：以国内外发表的论文为基础,经分析,归纳,从药理作用、体内过程、临床应用、副作用及产品发展概况等方面介绍洛伐他汀、普伐他汀、辛伐他汀、氟伐他汀及西伐他汀等药品。结果与结论：大量资料表明,ＨＭＧＣｏＡ 还原酶抑制剂是一类疗效显著、毒副作用少,耐受性好的理想降血脂药物,该类药的问世为治疗高胆固醇血症开辟了新途径。     

Adhesive mechanisms governing interferon-producing cell recruitment into lymph nodes

Natural interferon-producing cells (IPCs) are found in peripheral lymph nodes (PLNs), where they support NK cell, T cell, and B cell responses to pathogens. However, their route of entry and the adhesive mechanisms used to gain access to PLNs remain poorly defined. We report that IPCs can enter PLNs via a hematogenous route, which involves a multistep adhesive process, and that transmigration is enhanced by inflammation. Results indicate that L-selectin on IPCs is required for efficient attachment and rolling on high endothelial venules in vivo in both nonstimulated and inflamed PLNs. IPCs, however, also possess functional ligands for E-selectin that contribute to this process only in the latter case. In conjunction with selectin-mediated adhesion, both beta(1)- and beta(2)-integrins participate in IPC attachment to the inflamed vessel wall, whereas chemotaxis relies in part on the chemokine receptor CCR5. Identification of the adhesive machinery required for IPC trafficking into PLNs may provide opportunities to regulate immune responses reliant on the activity of these cells.     

1141644472Surface Enhanced Laser Desorption and Ionization (SELDI) mass spectrometry for investigation of human embryo conditioned IVF culture medium

Background:  Fetal programming is the notion that adverse environmental conditions in utero can cause short term survival adaptations that may have long-term consequences, such as chronic disease in subsequent lifetime. Recently, some authors reported that the increase of allergy prevalence in childhood may be linked with fetal immune development. In this regard, literature survey inspired to study the influence of two conjugated linoleic acid (CLA)-isomers (c9,t11 and t10,c12) on parameters of the immune system in pregnancy.
Methods:  Lymphocytes from allergic and non-allergic mothers, cord blood of their newborns, and the decidual layer of term placentae were isolated and cultured for 2 days and supplemented or not with relevant allergens, c9,t11 CLA, t10,c12 CLA or linoleic acid, respectively. Expression of CD69 (activation marker) and CD71 (transferrin receptor; proliferation marker) on B and T lymphocytes and T helper cell type 1 (Th1) and Th2 cytokines in culture supernatant were analyzed.
Results:  Both CLA isomers led to an increase of the IFN-γ/IL-10 ratio in supernatants of peripheral maternal and cord blood cells from allergic patients and non-allergic-control. CLA supplementation decreased IL-10 secretion of peripheral maternal and decidual lymphocytes and the portion of CD71⁺ maternal B cells. Linoleic acid induced a similar effect.
Conclusion:  An immunological effect of CLA on maternal and fetal lymphocyte responses could be demonstrated. It would be expected that further investigations could reveal differences in effects of CLA and linoleic acid.