论吴鞠通治疳学术思想

吴鞠通《温病条辨·解儿难》对疳疾的论治文短义深,所提治疳九妙法蕴藏着深奥的学术思想。本文从脾胃学说、升降出入学说、营卫学说等方面进行了阐发,以期能发掘其学术渊薮,指导学术研究及临床实践。     

健脾补肾利湿方对去卵巢大鼠主动脉晚期糖化终末产物(AGEs)的影响

为探讨健脾补肾利湿方对去卵巢（OVX）大鼠晚期糖化终末产物（AGEs）和过氧化的影响。雌性SD大鼠去除双侧卵巢2周后健脾补肾利湿方治疗13周。结果表明，与假手术组相比，去卵巢组主动脉AGEs、甘油三脂（TG）、氧化低密度脂蛋白（OX－LDL）和丙二醛（MDA）均明显升高，高密度脂蛋白－胆固醇（HDL－C）、载脂蛋白AⅠ（apo-AⅠ）和超氧化物歧化酶（SOD）活性均显降低。健脾补肾利湿方治疗组大鼠主动脉AGEs、血清TG、MDA和OX－LDL均明显低于去卵巢组；HDL－C、apo-AⅠ和SOD活性均显升高。提示健脾补肾利湿方通过降低去卵巢大鼠主动脉AGEs含量，降低大鼠血清OX－LDL和MDA含量，升高血清SOD活性，调节血脂，发挥其对心血管的保护作用。     

半夏厚朴汤及其组成中药抗焦虑和抑郁研究进展

目的：综述半夏厚朴汤及其组成中药抗焦虑和抑郁研究进展。方法：查阅国内外近年来有关文献结合相关的研究成果，从半夏厚朴汤对动物焦虑和抑郁行为、神经生化机制、免疫系统功能、血脂代谢、机体氧化防御系统等层次进行阐述。结果与结论：半夏厚朴汤及其组成中药抗焦虑和抑郁具有一定的科学性。     

Interleukin-6 activates phosphatidylinositol-3 kinase, which inhibits apoptosis in human prostate cancer cell lines

BACKGROUND: A number of recent studies have identified interleukin (IL)-6 as an important regulator of prostate cancer growth. Here, we investigate the potential interaction of IL-6 with phosphatidylinositol (PI)-3 kinase, a key growth regulatory enzyme, in prostate cancer cell lines. METHODS: Tyrosine phosphorylation of p85, the regulatory subunit of PI-3 kinase, in the human prostate cancer cell lines LNCaP and PC-3 was assessed by sequential immunoprecipitation with anti-p85 antibody and immunoblotting with anti-phosphotyrosine. The effects of wortmannin, an inhibitor of PI-3 kinase, and/or IL-6 on cell growth were assessed by MTT assays. DNA laddering experiments were performed to assay for programmed cell death. RESULTS: Tyrosine phosphorylation of p85 is upregulated by IL-6 in both LNCaP and PC-3. IL-6 promotes coprecipitation of p85 with gp130, the signal-transducing component of the IL-6 receptor. Inhibition of PI-3 kinase with wortmannin induces programmed cell death in PC-3 cells. In contrast, wortmannin has no effect on LNCaP growth when used alone; however, combined with IL-6, wortmannin promotes apoptosis in these cells. CONCLUSIONS: PI-3 kinase is involved in IL-6 signal transduction and delivers an antiapoptotic signal in human prostate cancer cell lines.     

Epithelioid trophoblastic tumor of the uterus in a postmenopausal woman: a case report and review of the literature

We report an epithelioid trophoblastic tumor (ETT), a recently delineated type of gestational trophoblastic tumor (GTT), discovered in the uterus of a 66-year-old woman. She had been treated for a hydatidiform mole 17 years previously without chemotherapy. The resected uterus contained a solid/cystic tumor located entirely within the myometrium. Microscopically, there was an epithelial-like growth pattern. The tumor was circumscribed, with a pushing border, and the tumor cells grew in cords, nests, and sheets within which were aggregates of hyaline material and necrotic debris. Most tumor cells were mononuclear and had an epithelioid appearance with distinct cell borders, eosinophilic cytoplasm, and nuclei with occasional indistinct nucleoli. Scattered multinucleated cells consistent with syncytiotrophoblastic cells were also present. Immunohistochemical staining revealed strong diffuse reactivity for cytokeratins (CK7, AE1/AE3, CAM 5.2, CK18) and epidermal growth factor receptor, and focal reactivity, mainly in syncytiotrophoblastic cells, for beta-human chorionic gonadotropin, human placental lactogen, and inhibin-alpha. The histologic and immunohistochemical features were characteristic of ETT, and helped to distinguish the tumor from other trophoblastic tumors and squamous cell carcinoma. An unusual observation was a high mitotic count, reflected in a Ki-67 proliferative index of 68.6%. Our findings indicate that ETT, like other types of GTT, can occur in postmenopausal women, even years after a gestational event.     

外周原始神经外胚层肿瘤形态学免疫表型及临床预后研究

目的：探讨外周原始神经外胚层肿瘤(PNET)的临床表现，病理及免疫组织化学特点及预后。方法：对15例外周原始神经外胚层肿瘤进行临床资料及预后，病理组织形态学和超微结构特点及免疫组化表型研究。结果：患者以男性为主，男女之比约为6．5：1，年龄6～35岁，年龄中位数为16，发生部位较多，可发生在直肠，腹膜后，腹股沟，淋巴结，胸壁，睾丸，鼻腔及骨组织等；免疫组化：肿瘤均弥漫表达CD99，并不同程度地表达NSE，SYN，CgA等，但不表达CK，Desmin，LCA等，其中1例表达组织化学染色PAS，随访最长时间为24月。结论：PNET是一种发生在年轻男性，进展迅速，预后非常差的恶性小圆细胞肿瘤，认识其临床病理特点及免疫组化表型对于该恶性肿瘤的诊断及临床治疗意义重大。     

穴注野木瓜治疗腰椎骨质增生108例疗效观察

方法：采用肾俞穴位注射野木瓜注射液治疗腰椎骨质增生108例，并没针刺对照组52例进行临床对照观察。结果：穴位注射有效率98％，对照组有效率85％。结论：野木瓜注射波穴位注射治疗腰椎骨质增生有显著疗效。