转化生长因子β1诱导骨髓间充质干细胞体外构建纤维蛋白胶软骨模块

目的:观察体外构建骨髓间充质干细胞、改良纤维蛋白胶及生长因子的组织工程软骨模块的可行性。方法:实验于2004-07/12在南方医科大学附属珠江医院中心实验室进行。选取新西兰兔10只,密度梯度法分离培养兔骨髓间充质干细胞,在培养系统中加入生长因子(含10μg/L转化生长因子β1,100mol/L地塞米松,50mg/L维生素C,以及1%ITS-A。培养第2,4,6,8天采用四甲基偶氮噻唑法在酶标仪上检测吸光度值表示细胞增殖。于培养7,14d行细胞爬片甲苯胺蓝染色及Ⅱ胶原免疫组化。诱导后骨髓间充质干细胞种植于改良纤维蛋白胶支架上,加入生长因子诱导培养,于培养7,14,21d行形态组织学及透射电镜检查。结果:实验兔10只均进入实验结果分析。①四甲基偶氮噻唑法测定培养第6和8天后,实验组骨髓间充质干细胞增殖的吸收度值高于对照组(实验组:0.4554±0.0206,0.5348±0.0169;对照组:0.4270±0.0255,0.5057±0.0368,P<0.05)。②细胞爬片甲苯胺蓝染色细胞周围有蓝色基质。Ⅱ型胶原免疫组化染色阳性。③诱导后骨髓间充质干细胞在改良纤维蛋白胶支架中培养,Masson染色可见胞浆及细胞周围有胶原的形成;Ⅱ型胶原免疫组化染色阳性。透射电镜可见细胞代谢旺盛。结论:骨髓间充质干细胞、改良纤维蛋白胶及生长因子的组织工程模块体外培养系统中,改良纤维蛋白胶支架相容性好,转化生长因子β等生长因子促进骨髓间充质干细胞在改良纤维蛋白胶支架增殖,诱导分化成软骨细胞表型。     

郭志强教授运用崇阳理论治疗反复胚胎移植着床失败的经验

本文介绍郭志强教授运用崇阳理论治疗反复胚胎移植着床失败的经验.郭老认为妇人体质"阴常不足,阳亦常虚",形成"阴阳相济,阴基阳主"的崇阳理论.认为反复胚胎移植着床失败的主要病机为肾阳不足,胞宫寒冷,不能摄精成孕,提出治疗应从阳气着手,并秉持着求子先调经的原则,在胚胎移植前采用中药序贯疗法调经促孕,胚胎移植后予两固汤补肾温阳,顾护阳气,促进着床.     

广东粤西地区汉族人群红细胞酸性磷酸酶-腺苷脱氨酶-腺苷酸激酶的遗传学特性

目的:应用红细胞酸性磷酸酶-腺苷脱氨酶-腺苷酸激酶同步电泳分型,从血型遗传学角度对粤西汉族血液中3种酶型的表型分布、基目频率进行调查。方法:实验于2005-03/11在湛江师范学院完成。①血样取自广东湛江地区无血缘关系的汉族健康成人120例,3代内无与外族通婚史。②120例汉族健康成人均取耳血一两滴,渗于无菌纱布上,血痕直径1cm,低温干燥保存,7～14d内进行血痕表型检测。③基因频率计算采用Hardy-Weinbery平衡计算法。种群中某基因频率(%)=种群中该基因总数/种群中该对等位基因总数×100%;种群中某基因型频率(%)=种群中该基因型个体数/该种群的个体数×100%。结果:实验血样取自广东湛江地区无血缘关系的汉族健康成人120例,全部进入结果分析。①粤西汉族酸性磷酸酶、腺苷脱氨酶、腺苷酸激酶3种酶型表现型的观察值与期望值基本吻合(P>0.05),检测的酶型中未发现稀有型、变异型,亦未发现罕见基因。②在粤西汉族群体中,酸性磷酸酶具有中高鉴别能力(积累能力值0.5043),腺苷脱氨酶为低鉴别能力(积累能力值0.2922),而腺苷酸激酶无鉴别能力(积累能力值0.0023)。结论:粤西汉族人群3种检测酶型中未发现稀有型、变异型,亦未发现罕见基因。符合Hardy-Weinbery遗传平衡定律。     

ATP binding cassette G8 T400K polymorphism may affect the risk of gallstone disease among Chinese males

BACKGROUND: Supersaturation of bile with cholesterol is a primary step in the formation of cholesterol gallstones. ATP binding cassette (ABC) G5 and G8 play an important role in regulating sterol absorption and secretion. To investigate a possible association between transporter gene polymorphism and gallstone formation, we examined five common polymorphisms in the ABCG5 (Q604E) and ABCG8 (D19H, Y54C, T400K, A632V) genes in patients with gallstone disease (GS). METHODS: Study subjects included 287 patients with GS and 219 gallstone free controls (GSF). Polymorphisms were determined using PCR-RFLP analysis or the Taqman MGB assay. Plasma and biliary lipid levels were measured. RESULTS: 2 SNPs of ABCG8 gene (Y54C and T400K) showed strong linkage disequilibrium (D'=0.824, r2=0.579). Male carriers of the less frequent K allele of ABCG8 T400K had a 2.31-fold elevated risk [95% confidence interval (CI) 1.12 approximately 4.76, P=0.023] for gallstone disease compared to male with the common genotype after the adjustment for age, body mass index. Males with the K allele had lower plasma triglyceride (P=0.044) and biliary phospholipid (P=0.035) levels than TT homozygotes. No such association was found in female or other 4 SNPs. CONCLUSIONS: These findings indicate that the T400K polymorphism in ABCG8 may be associated with the incidence of gallstone disease in males.     

Computer-Delivered Social Norm Message Increases Pain Tolerance

Background

Few experimental studies have been conducted on social determinants of pain tolerance.

Purpose

This study tests a brief, computer-delivered social norm message for increasing pain tolerance.

Methods

Healthy young adults (N?=?260; 44 % Caucasian; 27 % Hispanic) were randomly assigned into a 2 (social norm)?×?2 (challenge) cold pressor study, stratified by gender. They received standard instructions or standard instructions plus a message that contained artifically elevated information about typical performance of others.

Results

Those receiving a social norm message displayed significantly higher pain tolerance, F(1, 255)?=?26.95, p?<?.001, η _p ² ?=?.10 and pain threshold F(1, 244)?=?9.81, p?=?.002, η _p ² ?=?.04, but comparable pain intensity, p?>?.05. There were no interactions between condition and gender on any outcome variables, p?>?.05.

Conclusions

Social norms can significantly increase pain tolerance, even with a brief verbal message delivered by a video.     

New method of thrombus preparation using a fluid model for evaluation of thrombectomy devices in a swine model

Background

Mechanical thrombectomy is a promising new modality of interventional stroke treatment. Preparation of thrombus is a very important step for the evaluation of the mechanical thrombectomy devices. The objective of this study was to explore a new method of thrombus preparation with fluid model (FM) for assessment of thrombectomy devices used in the recanalization of acute ischemic stroke.

Methods

Elongation test and catheter injection test were used to evaluate the mechanical properties of thrombi prepared by FM and static model (SM). Histological structures of two artificial clots and specimens of stroke patients were compared. Radiopacity of thrombus made by FM was evaluated in a swine embolization model.

Results

The maximum tensile length of thrombi prepared by FM and SM were significantly higher (4.28 ± 0.23 cm vs 3.16 ± 0.13 cm, P < 0.01) and showed less breakage on catheter injection test (13% vs 60%, P < 0.05). Histological features of thrombi prepared by FM showed mixed thrombus structure, similar to thromboemboli retrieved from acute stroke patients, while clots generated by SM were replete with erythrocytes. A total of twelve vessels in two swine were successfully occluded (TIMI 0 or 1), with sufficient radiopacity of each injected thrombus.

Conclusion

The thrombus prepared by FM had good mechanical stability, sufficient radiopacity, and similar histological structure of thromboemboli retrieved from stroke patients, which make it possible to be used in the evaluation of thrombectomy devices.     

多普勒超声测定左心室Max（dp／dt）的新方法

心室内压力变化率峰值Ｍａｘ（ｄｐ／ｄｔ）是一项评价心肌收缩功能的重要指标。但其测定常需借助于心导管术，临床应用受到限制。依据流体力学基本定律，本文提出：等容收缩期内表现为压力的势能将在射血期部分地转化为以１／２ｐＶ２为形式的动能，因而势能的最大变化率Ｍａｘ（ｄｐ／ｄｔ）将在动能的最大变化率Ｍａｘ（ｄｋ／ｄｔ）上得到反映。应用特制计算机软件将主动脉血流速度曲线转化为４Ｖ２曲线，该曲线的一级导数峰值即为Ｍａｘ（ｄｋ／ｄｔ）。应用脉冲多普勒超声测定２３例先天性心脏病患儿的主动脉血流频谱参数并与心导管测定的左心室Ｍａｘ（ｄｐ／ｄｔ）进行对比分析。结果显示：主动脉血流频谱参数Ｍａｘ（ｄｋ／ｄｔ）与左心室Ｍａｘ（ｄｐ／ｄｔ）呈良好相关（ｒ＝０．８１７，Ｐ＜０．００１），直线回归方程为：Ｍａｘ（ｄｐ／ｄｔ）＝０．４７Ｍａｘ（ｄｋ／ｄｔ）＋６７．９（Ｋｐａ／ｓ），平均加速度ｍＡ及Ｖｐ２／ＡＴ与Ｍａｘ（ｄｐ／ｄｔ）亦有较好的相关性。表明由多普勒超声获得的主动脉血流频谱参数Ｍａｘ（ｄｋ／ｄｔ）能较准确地估侧左室Ｍａｘ（ｄｐ／ｄｔ），因而可作为评价心脏收缩功能的可靠指标