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排序方式: 共有380条查询结果,搜索用时 15 毫秒
331.
De nombreux analgésiques sont métabolisés par l’intermédiaire des iso-enzymes du cytochrome P450 (CYP) dont notamment le CYP2D6
soumis à un polymorphisme génétique. Les conséquences cliniques vont de la toxicité médicamenteuse à l’absence d’efficacité
selon l’analgésique et le polymorphisme considérés. En détectant les polymorphismes génétiques par génotypage et/ou phénotypage,
la pharmacogénétique permettra de mieux individualiser l’approche analgésique médicamenteuse et ainsi améliorer la sécurité
et l’efficacité de nombreux antalgiques. 相似文献
332.
Steven R. Goldring Jean-Michel Dayer Stephen M. Krane 《Calcified tissue international》1979,29(1):193-200
Summary Cells dispersed from human giant cell tumors of bone and grown in monolayer culture increase intracellular cyclic AMP (cAMP)
when incubated with parathyroid hormone (PTH) or prostaglandin E2 (PGE2). When cells are continuously exposed to PTH, cAMP levels increase acutely but then decrease rapidly to pretreatment values
despite continued presence of hormone or addition of new hormone. Preincubation of cells with PTH for periods as short as
10 min results in a decrease in the capacity of cells to increase cAMP content when re-exposed to maximal stimulatory concentrations
of PTH. The decrease in the magnitude of the PTH-induced cAMP response observed in cells pretreated with this hormone is dependent
on the concentration of PTH present during the pre-incubation. The loss of cAMP response in cells pre-treated with either
PGE2 or PTH is hormone specific in that cells made refractory by pretreatment with one hormone still increase cAMP content when
exposed to the other. Although the cells are not releasing measurable amounts of prostaglandins into the medium, pretreatment
with indomethacin results in an increase in the magnitude of the cAMP response to PGE2. The PTH-induced cAMP response is not affected by indomethacin pre-treatment. The loss of PTH responsiveness produced by
hormone preincubation is consistent with the phenomenon of “down-regulation” observed with ligand-receptor interactions in
a variety of tissues. 相似文献
333.
334.
Summary Since the magnitude of the response to a drug may depend upon the drug input rate, the concentration-effect relationship of the new dihydropyridine (±)-isradipine was investigated using different administration modalities.Ten normotensive healthy volunteers were given, double-blind and in a crossover fashion, isradipine as a 1 mg iv infusion, 5 mg oral solution, 5 mg standard tablet, 10 mg slow release formulation, and a placebo. Blood pressure, heart rate, and plasma isradipine concentrations were recorded for 24 h.The maximal fall in diastolic blood pressure was similar after the infusion (-11.40 mmHg), the oral solution (-15.20 mmHg), and the standard tablet (-12.50 mmHg). In healthy volunteers the slow release form had no significant effect on blood pressure. The concentration-effect plots showed an increasing slope in the order infusion, solution, and tablet, and anticlockwise hysteresis. This was partly due to marked heart rate counter-regulation, the corresponding mean maximal heart rate increases being 24, 19, and 17 beats·min–1.The pronounced counter-regulation of the heart rate implies that a slow isradipine input rate would be more effective in decreasing blood pressure. 相似文献
335.
B Krauer H Nau P Dayer P Bischof R Anner 《British journal of obstetrics and gynaecology》1986,93(4):322-328
Paired samples of maternal and fetal serum were obtained in 65 pregnancies between 13 and 41 weeks gestation. Concentrations of albumin, alpha 1-acid glycoprotein (alpha 1-AGP) and free fatty acids (FFA) were estimated in all samples and the in-vitro binding of diazepam and propranolol was estimated. The free fraction of diazepam in fetal serum was very high in early gestation and decreased with advancing gestation to reach maternal values around the 30th week. After 35 weeks gestation, values were generally lower in fetal than in maternal serum. Fetal serum binding of diazepam correlated with fetal albumin levels and gestational age. The ratio of the fetal/maternal free diazepam fraction correlated negatively with the fetal/maternal serum albumin concentration ratio. The maternal/fetal free diazepam fraction ratio correlated positively with gestational age. The free fraction of propranolol in fetal serum was higher than in maternal serum in early pregnancy and decreased with advancing gestation but always remained higher than the maternal values. The fetal/maternal free propranolol fraction ratio was negatively correlated with the fetal/maternal alpha 1-AGP serum concentration ratio. The maternal/fetal free propranolol fraction ratio was positively correlated with gestational age. Serum protein binding of propranolol was correlated with serum albumin concentrations in fetal samples but not in maternal samples. Serum FFA concentrations were not correlated with serum protein binding except for maternal serum binding of diazepam. For toxicological reasons it is advisable to monitor maternal blood for free concentrations of drugs that exhibit concentration-dependent protein binding or which can be displaced from binding sites by other drugs or endogenous compounds such as FFA. 相似文献
336.
Summary Although clonidine analgesia appears to be mediated by the same central 2-adrenoceptors that mediate its hypotensive effect, it is short-lasting when compared to the fall in blood pressure. This has been investigated by combined pharmacokinetic-pharmacodynamic analysis in 10 healthy volunteers who received (double-blind and crossover) clonidine 200 g orally + placebo i.v. and clonidine orally + naloxone i.v. (2.8 mg/5 h). Analgesia was assessed by measuring the subjective (VAS) and objective (RIII) pain thresholds after transcutaneous electrical stimulations of the sural nerve; the mean arterial blood pressure (MAP), salivary flow (SF), and plasma clonidine concentrations were also monitored. A combined pharmacokinetic (first order absorption — 1 compartment) — pharmacodynamic (linear) model, including a hypothetical effect compartment with and without tolerance, were fitted to the data.Clonidine and clonidine + naloxone increased subjective and objective pain thresholds for 4 h. The concentration-effect plot for MAP showed distinct hysteresis. The t1/2s for effect compartment equilibration were 29 and 42 min for clonidine + naloxone and clonidine. The concentration-effect curves for RIII had the same shape as MAP but the starting hysteresis suddenly collapsed, suggesting acute tolerance. The best fit was obtained with a model where the linear relationship between concentration in the effect compartment and analgesia changed acutely after the third hour.The short-lived analgesia was probably related to an acute change in pain sensitivity induced by food, suggesting that it is not mediated solely by the 2-adrenoceptors responsible for hypotension.Presented in part at the XIth International Congress of Pharmacology, Amsterdam, July 1990 相似文献
337.
338.
J W Mills A D Macknight J M Dayer D A Ausiello 《The American journal of physiology》1979,236(3):C157-C162
Pig kidney cells, LLC-PK1, grown by standard tissue-culture techniques form monolayers and maintain morphological features characteristic of epithelia. Cultures exposed to 2 X 10(-6) M [3H]ouabain for 30 min at 37 degrees C bound 7.77 +/- 0.37 pmol/mg protein. This could be reduced by 58% by incubation in the presence of 45 mM K+. Freeze-dry radioautographic localization of [3H]ouabain-binding sites revealed grains distributed only along that fraction of the plasmalemma directly facing the culture-dish surface. Binding and localization of [3H]ouabain were correlated with an inhibition of the Na+ pump in these cells because analysis of cellular electrolytes in control cultures versus those exposed to 10(-3) M ouabain revealed a fall in K+ from 419 +/- 9 to 173 +/- 4 mmol/kg dry wt with a reciprocal increase in Na+. There was no change in cell H2O. Similarly, oxygen consumption was reduced by 32% after exposure to ouabain. These results provide direct evidence that in epithelial cells in culture the membrane facing the culture dish corresponds to the basolateral membrane of epithelial cells in vivo. 相似文献
339.
Effect of mechanical ventilation on inflammatory mediators in patients with acute respiratory distress syndrome: a randomized controlled trial. 总被引:67,自引:0,他引:67
V M Ranieri P M Suter C Tortorella R De Tullio J M Dayer A Brienza F Bruno A S Slutsky 《JAMA》1999,282(1):54-61
CONTEXT: Studies have shown that an inflammatory response may be elicited by mechanical ventilation used for recruitment or derecruitment of collapsed lung units or to overdistend alveolar regions, and that a lung-protective strategy may reduce this response. OBJECTIVE: To test the hypothesis that mechanical ventilation induces a pulmonary and systemic cytokine response that can be minimized by limiting recruitment or derecruitment and overdistention. DESIGN AND SETTING: Randomized controlled trial in the intensive care units of 2 European hospitals from November 1995 to February 1998, with a 28-day follow-up. PATIENTS: Forty-four patients (mean [SD] age, 50 [18] years) with acute respiratory distress syndrome were enrolled, 7 of whom were withdrawn due to adverse events. INTERVENTIONS: After admission, volume-pressure curves were measured and bronchoalveolar lavage and blood samples were obtained. Patients were randomized to either the control group (n = 19): tidal volume to obtain normal values of arterial carbon dioxide tension (35-40 mm Hg) and positive end-expiratory pressure (PEEP) producing the greatest improvement in arterial oxygen saturation without worsening hemodynamics; or the lung-protective strategy group (n = 18): tidal volume and PEEP based on the volume-pressure curve. Measurements were repeated 24 to 30 and 36 to 40 hours after randomization. MAIN OUTCOME MEASURES: Pulmonary and systemic concentrations of inflammatory mediators approximately 36 hours after randomization. RESULTS: Physiological characteristics and cytokine concentrations were similar in both groups at randomization. There were significant differences (mean [SD]) between the control and lung-protective strategy groups in tidal volume (11.1 [1.3] vs 7.6 [1.1] mL/kg), end-inspiratory plateau pressures (31.0 [4.5] vs 24.6 [2.4] cm H2O), and PEEP (6.5 [1.7] vs 14.8 [2.7] cm H2O) (P<.001). Patients in the control group had an increase in bronchoalveolar lavage concentrations of interleukin (IL) 1beta, IL-6, and IL-1 receptor agonist and in both bronchoalveolar lavage and plasma concentrations of tumor necrosis factor (TNF) alpha, IL-6, and TNF-alpha, receptors over 36 hours (P<.05 for all). Patients in the lung-protective strategy group had a reduction in bronchoalveolar lavage concentrations of polymorphonuclear cells, TNF-alpha, IL-1beta, soluble TNF-alpha receptor 55, and IL-8, and in plasma and bronchoalveolar lavage concentrations of IL-6, soluble TNF-alpha receptor 75, and IL-1 receptor antagonist (P<.05). The concentration of the inflammatory mediators 36 hours after randomization was significantly lower in the lung-protective strategy group than in the control group (P<.05). CONCLUSIONS: Mechanical ventilation can induce a cytokine response that may be attenuated by a strategy to minimize overdistention and recruitment/derecruitment of the lung. Whether these physiological improvements are associated with improvements in clinical end points should be determined in future studies. 相似文献
340.
J. C. Krayenbühl S. Vozeh M. Kondo-Oestreicher P. Dayer 《European journal of clinical pharmacology》1999,55(8):559-565
Introduction: Mibefradil was approved as a novel calcium antagonist in Switzerland in 1996. Following its launch as an antihypertensive
and anti-anginal agent, there were reports about serious pharmacokinetic and pharmacodynamic interactions occurring with other
drugs frequently administered to patients with cardiovascular diseases. Despite appropriate modifications of the prescribing
information, such interactions continued to occur. The drug was finally withdrawn after a study in patients with congestive
heart failure showed a trend to higher mortality with mibefradil. This increase in mortality could again be due to multiple
interactions between mibefradil and other drugs. In retrospect, it can be concluded that several of the interactions, including
the theoretical risk of severe toxicity in some patients, could have been and in fact were predicted on the basis of the data
available before introduction to the market. Depending on the benefits, these problems would however not necessarily represent
an unacceptable risk for a new active compound.
Results and conclusion: The most important points revealed by this analysis were: (1) when interpreting the results of interaction studies, it is
important to consider not only the mean of the interaction effect but also the observed and the theoretically conceivable
extreme effects in individual subjects and (2) a drug with a high interaction potential may represent a high risk even if
an adequate warning is included in the product information. The need for specific pharmacokinetic and pharmacodynamic interaction
studies with new drugs and the limitations of the pivotal clinical efficacy and safety studies during phase III in order to
reveal such interactions are discussed.
Received: 19 March 1999 / Accepted in revised form: 29 July 1999 相似文献