傣中医结合治疗咳嗽37例疗效观察

目的：观察傣中医结合治疗咳嗽的临床疗效。方法：采用中药麻杏三子灯台汤加减，配合傣药棉榔青止咳糖浆内服治疗3天后观察疗效。结果：治愈28例，好转9例，总有效率100％。结论：傣中医结合治疗咳嗽有较好疗效。     

宫颈癌及子宫内膜癌细胞系中hTERT启动子活性的研究

目的 :探讨Hela及HHUC细胞系中hTERT启动子的活性及其与hTERTmRNA表达和端粒酶活性之间的关系。方法 :将hTERT核心启动子基因转入Hela、HHUC细胞系、人正常皮肤表皮细胞及子宫内膜上皮细胞中 ,检测细胞系hTERT启动子的活性、hTERTmRNA表达水平及端粒酶活性。分别以HEK2 93细胞系作为阳性对照 ,HELF细胞系作为阴性对照。结果 :细胞系Hela、HHUC、人正常皮肤表皮细胞及正常子宫内膜上皮细胞中的hTERT启动子活性分别为 2 0 1%、14 9%、0 3%和 0 5 % ;hTERTmRNA相对表达水平分别为 0 6 3、0 5 1、0和 0 ;端粒酶活性分别为 0 393、0 387、0 14 4和 0 15 2。结论 :宫颈癌细胞系及子宫内膜癌细胞系中hTERT启动子活性、hTERTmRNA表达水平和端粒酶活性均特异性增高 ,而在正常角化细胞及子宫内膜上皮细胞中则被抑制或不表达。hTERT启动子活性水平与hTERTmRNA表达水平和端粒酶活性之间有显著相关性     

The AFT024 stromal cell line supports long-term ex vivo maintenance of engrafting multipotent human hematopoietic progenitors.

The immortalized murine stromal cell line AFT024 has been reported to maintain human hematopoietic progenitors in an undifferentiated state in vitro. In the current studies the beige/nude/xid (bnx) mouse in vivo xenograft model was used to examine the engraftment and multilineage generative potential of human hematopoietic progenitors after 2-3 weeks growth on AFT024 stroma, in comparison to primary stromal monolayers derived from post-natal human bone marrow. Eight to 12 months after transplantation of human CD34+CD38- cells from umbilical cord blood, cultured on AFT024 vs human stroma for 2-3 weeks, the murine bone marrow was harvested and analyzed for the presence of human myeloid and lymphoid cells. The mean percent engraftment of total human hematopoietic cells in the murine marrow was significantly higher after co-cultivation on AFT024 than on human stroma. Human myeloid and lymphoid lineage cells were detected in all mice. However, engraftment of myeloid lineage cells (CD33+), B lymphoid (CD19+), and T lymphoid cells (CD4+and CD8+) were significantly higher after co-cultivation of the human cells on AFT024 than on human stroma, prior to transplantation. Interestingly, the length of time in culture did not significantly affect the engraftment of the myeloid and T lymphoid lineage progenitors, but the percentage of B lymphoid lineage engraftment decreased significantly between 2 and 3 weeks of co-cultivation on both types of stroma. Cells with a primitive phenotype (CD45+/CD34-/CD38- and CD45+/CD34-/lin-) and cells with the capacity to generate secondary human CFU after recovery from the bnx bone marrow were maintained at significantly higher levels during culture on AFT024 stroma than on human stroma. The current studies demonstrate that the AFT024 murine stromal cell line supports the ex vivo survival and maintenance of human hematopoietic progenitors that are capable of long-term multilineage reconstitution for 2-3 weeks ex vivo, to levels superior to those that can be obtained using human stromal cells.     

提取丹参酮ⅡA 的工艺探讨

目的：提高丹参有效成分的提出率。方法：将提取丹参常用的乙醇提取法与超临界萃取法进行比较。结果：超临界萃取技术提出率明显高于乙醇提取法。结论：超临界萃取技术在丹参的提取中可适当推广应用。     

Percutaneous screw fixation or cast immobilization for nondisplaced scaphoid fractures

BACKGROUND: Nondisplaced scaphoid fractures treated with prolonged cast immobilization may result in temporary joint stiffness and muscle weakness in addition to a delay in return to sports or work. Fixation of scaphoid fractures with a percutaneous cannulated screw has resulted in a shorter time to union and to return to work or sports. The purpose of this prospective, randomized study was to compare cast immobilization with percutaneous cannulated screw fixation of nondisplaced scaphoid fractures with respect to time to radiographic union and to return to work. METHODS: Twenty-five full-time military personnel with an acute nondisplaced fracture of the scaphoid waist consented to be randomized to either cast immobilization or fixation with a percutaneous cannulated Acutrak screw (Acumed, Beaverton, Oregon) for the purpose of this study. Time to fracture union, wrist motion, grip strength, and return to work as well as overall patient satisfaction at the time of a two-year follow-up were evaluated. RESULTS: Eleven patients were randomized to percutaneous cannulated screw fixation, and fourteen were randomized to cast immobilization. The average time to fracture union in the screw fixation group was seven weeks compared with twelve weeks in the cast immobilization group (p = 0.0003). The average time until the patients returned to work was eight weeks compared with fifteen weeks in the cast immobilization group (p = 0.0001). There was no significant difference in the range of motion of the wrist or in grip strength at the two-year follow-up evaluation. Overall patient satisfaction was high in both groups. CONCLUSIONS: Percutaneous cannulated screw fixation of nondisplaced scaphoid fractures resulted in faster radiographic union and return to military duty compared with cast immobilization. The specific indications for and the risks and benefits of percutaneous screw fixation of such fractures must be determined in larger randomized, prospective studies.     

结核分枝杆菌Ag85B-MPT64融合基因的构建表达

目的：构建结核分枝杆菌(H37Rv)Ag85B-MPT64融合基因并在真核细胞中表达．方法：采用序贯PCR(geneSOEing)法将Ag85B和MPT64编码基因用疏水甘氨酸接头(Gly4Ser)3经PCR扩增融合，定向克隆入pcDNA3．1( )中。采用脂质体转染法将pcDNA／Ag85B—MPT64(pcDNA／AM)转染COS-7细胞，用RT-PCR、ELISA和斑点印迹法检测其表达。结果：Ag85B-MPT64融合基因经双向DNA序列测定，碱基突变率为0．11％(2／1707)，突变为无意义突变；重组质粒转染COS-7细胞后经检测证实，该融合基因能在真核细胞中表达。结论：成功地构建了Ag85B-MPT64融合基因并在真核细胞中表达，融合蛋白具有良好的抗原性。     

Reduction in levels of the cyclin-dependent kinase inhibitor p27kip-1 coupled with transforming growth factor β neutralization induces cell-cycle entry and increases retroviral transduction of primitive human hematopoietic cells

Successful gene therapy depends on stable transduction of hematopoietic stem cells. Target cells must cycle to allow integration of Moloney-based retroviral vectors, yet hematopoietic stem cells are quiescent. Cells can be held in quiescence by intracellular cyclin-dependent kinase inhibitors. The cyclin-dependent kinase inhibitor p15^INK4B blocks association of cyclin-dependent kinase (CDK)4/cyclin D and p27^kip-1 blocks activity of CDK2/cyclin A and CDK2/cyclin E, complexes that are mandatory for cell-cycle progression. Antibody neutralization of β transforming growth factor (TGFβ) in serum-free medium decreased levels of p15^INK4B and increased colony formation and retroviral-mediated transduction of primary human CD34⁺ cells. Although TGFβ neutralization increased colony formation from more primitive, noncycling hematopoietic progenitors, no increase in M-phase-dependent, retroviral-mediated transduction was observed. Transduction of the primitive cells was augmented by culture in the presence of antisense oligonucleotides to p27^kip-1 coupled with TGFβ-neutralizing antibodies. The transduced cells engrafted immune-deficient mice with no alteration in human hematopoietic lineage development. We conclude that neutralization of TGFβ, plus reduction in levels of the cyclin-dependent kinase inhibitor p27, allows transduction of primitive and quiescent hematopoietic progenitor populations.     

多波长数据线性双组合同步荧光法测定苯酚与间苯二酚

提出了多波长数据线性双组合法计算解析苯酚和间苯二酚混合物的同步荧光光谱,考察了△λ=λ_em-λ_ex,pH,光路中光照时间对二种酚的荧光强度影响,建立了同时测定混合物中苯酚和间苯二酚的新方法。用于复方雷琐辛涂剂中苯酚与间苯二酚的同时测定,平均回收率分别为:苯酚98.5%±1.4%;间苯二酚98.8%±1.3%。     

Infiltrating hematoma of the hepatic pedicle and gallbladder wall. Ultrasonographic and x-ray computed tomographic aspects

A case of infiltrative hematoma of the porta hepatitis and the gallbladder wall is reported. It is a rare complication of anticoagulant treatment. The sonographics findings were 1 degree an echogenic material surrounding the porta hepatis, and 2 degrees a gallbladder wall thickening. Both these lesions appeared as dense areas on computed tomography (CT) scan. The aetiologies of hemorrhagic cholecystitis are discussed.     

Endothelial denudation and myointimal thickening in the rat carotid artery induced by the passage of bubbles

A new technique for the selective removal of endothelium in the rat carotid artery has been developed, and subsequent events in the vascular wall have been examined. To achieve de-endothelialization, more than 2,000 bubbles of nitrogen in phosphate-buffered saline are passed through a temporarily isolated segment of rat carotid over a period of 3 min. Bubbles are generated by a simple apparatus, consisting of a pressurized tilting chamber and catheter. Endothelium is removed while subendothelial basement membrane and other subjacent structures remain intact. Platelets attach to the denuded surface within minutes after re-establishment of blood flow. Myointimal thickening is found at 5 weeks and 4 months after de-endothelialization. The method is quite reliable and will facilitate further studies of reactions to carotid endothelial injury in young adult rats.