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Hozumi K Ohtsuka R Suzuki D Ando K Ito M Nishimura T Merkenschlager M Habu S 《Immunology letters》2000,71(1):61-66
To overcome low efficiency of retroviral infection into immature T cells, we modified reaggregation fetal thymus organ culture by closely packed co-culture with virus-producing cells (VPC). The viral vector was constructed in chimeric vector, pMX, with IRES and tailless-rat CD2 as a surface marker of infected cells. A rearranged TCR beta gene (Vbeta8.2) was further inserted into the construct for investigating effect of the introduced gene in T cell development. Using this system, we succeeded to transfer the viral vector into immature thymocytes at a remarkably higher efficiency compared to conventional methods using medium containing retrovirus. Moreover, the introduced TCR beta gene was expressed on thymocytes of RAG2-deficient mice to induce in the transition of CD4-CD8- double-negative (DN) into CD4+CD8+ double-positive (DP) cells by transducing beta-selection signaling. Thus, our modified reaggregation culture system is useful for studying the molecular mechanism of T cell development due to a highly efficient gene transfer into immature T cells. 相似文献
13.
The Serum Factor from Patients with Ulcerative Colitis that Induces T Cell Proliferation in the Mouse Thymus Is Interleukin-7 总被引:3,自引:0,他引:3
Mamoru Watanabe Noriaki Watanabe Yasushi Iwao Haruhiko Ogata Takanori Kanai Yoshitaka Ueno Masaharu Tsuchiya Hiromasa Ishii Sadakazu Aiso Sonoko Habu Toshifumi Hibi 《Journal of clinical immunology》1997,17(4):282-292
The disturbance of immune regulatory T cells is related to the pathogenesis of ulcerative colitis. Here we demonstrated and characterized the serum factor from ulcerative colitis patients that induced proliferation of intrathymic T cells. The factor isolated from the patient sera by a combination of gel filtration and anion-exchange chromatography induced proliferation of CD4+CD8– intrathymic T cells in the organ-cultured embryonic mouse thymus. Purification and amino acid sequence analysis of the serum factor demonstrated that the N-terminal 12 sequence was homologous to that of interleukin-7. SDS-PAGE and Western blot confirmed that purified serum factor was interleukin-7. Enzyme immunoassay demonstrated that the serum interleukin-7 concentration was significantly increased in the patients. PCR and Southern blot hybridization demonstrated that interleukin-7 mRNA expression was increased in the thymus tissues from patients but decreased in the colonic mucosa. Since interleukin-7 is a crucial cytokine for proliferation and differentiation of T cells in the thymus, the present study indicates that interleukin-7 may contribute to the disturbance of immune regulatory T cells in ulcerative colitis. 相似文献
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Tamauchi H Terashima M Ito M Maruyama H Ikewaki N Inoue M Gao X Hozumi K Habu S 《International immunology》2004,16(1):179-187
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Fumie Takewaki Hanako Nakajima Daiki Takewaki Yoshitaka Hashimoto Saori Majima Hiroshi Okada Takafumi Senmaru Emi Ushigome Masahide Hamaguchi Masahiro Yamazaki Yoshiki Tanaka Shunji Nakajima Hiroshi Ohno Michiaki Fukui 《Nutrients》2021,13(6)
The aim of this research was to reveal the characteristics of gut microbiome altered by acarbose intervention in Japanese patients with type 2 diabetes (T2D) and its possible association with habitual dietary intake. Eighteen patients with T2D were administered acarbose for four weeks. The abundances of two major phyla, namely Actinobacteria and Bacteroidetes, were reciprocally changed accompanied by the acarbose intervention. There were also significant changes in the abundances of ten genera, including the greater abundance of Bifidobacterium, Eubacterium, and Lactobacillus and the lower abundance of Bacteroides in the group after the intervention than that before the intervention. Hierarchical clustering of habitual dietary intake was performed based on the pattern of changes in the gut microbiota and were classified into distinct three clusters. Cluster I consisted of sucrose, cluster II mainly included fat intake, and cluster III mainly included carbohydrate intake. Moreover, the amount of change in Faecalibacterium was positively correlated with the intake of rice, but negatively correlated with the intake of bread. The intake of potato was negatively correlated with the amount of change in Akkermansia and Subdoligranulum. Acarbose altered the composition of gut microbiome in Japanese patients with T2D, which might be linked to the habitual dietary intake. 相似文献
17.
Masayuki Tomioka Tohru Yoneyama Yuki Tobisawa Kota Kawase Chie Nakai Manabu Takai Daiki Kato Koji Iinuma Keita Nakane Kosuke Mizutani Yasuhiro Hashimoto Takuya Koie 《Translational andrology and urology》2021,10(3):1192
BackgroundAlthough the patients with muscle-invasive bladder cancer (MIBC) generally have poor prognosis, the utility of these biomarkers for the prediction of oncological outcomes in MIBC has not been completely explored. Ghrelin regulates processes associated with cancer, including cell proliferation, apoptosis, cell migration, cell invasion, and angiogenesis. Thus, we aimed to evaluate the impact of serum ghrelin levels on survival in MIBC.MethodsIn this study, we reviewed the clinical and pathological records of 56 patients who were diagnosed with MIBC between November 2015 and November 2019 at Gifu and Hirosaki University Hospitals. We focused on 27 patients who had received chemotherapy and collected blood samples before and after chemotherapy. Blood samples were collected before chemotherapy and after completing two cycles of chemotherapy. Serum acyl (AG) and desacyl ghrelin (DG) were measured using AG and DG enzyme-linked immunosorbent assay kits (SCETI, Tokyo, Japan), respectively.ResultsThe 3-year overall and progression-free survival (PFS) rates were 82.9% and 68.3%, respectively. According to the AG level after chemotherapy, the 3-year PFS rates were 77.5% and 53.0% in patients with AG levels ≥1.34 and <1.34 pg/mL, respectively (P=0.038). With regard to DG levels after chemotherapy, the 3-year PFS rates were 90.9% and 43.3% in patients with DG levels <92.3 and ≥92.3 pg/mL, respectively (P=0.039). On multivariate analysis, serum AG levels were significantly associated with PFS.ConclusionsThis study suggested the usefulness of the ghrelin as a prognostic predictor of PFS in patients with MIBC. 相似文献
18.
Hozumi Katsuto; Kobori Akiko; Sato Takehito; Nishimura Takashi; Habu Sonoko 《International immunology》1996,8(10):1473-1481
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Real‐world efficacy and safety of daclatasvir and asunaprevir therapy for hepatitis C virus‐infected cirrhosis patients 下载免费PDF全文
Kei Morio Michio Imamura Yoshiiku Kawakami Reona Morio Tomoki Kobayashi Satoe Yokoyama Yuko Nagaoki Tomokazu Kawaoka Masataka Tsuge Akira Hiramatsu Grace Naswa Makokha C Nelson Hayes Hiroshi Aikata Daiki Miki Hidenori Ochi Yoji Honda Nami Mori Shintaro Takaki Keiji Tsuji Kazuaki Chayama 《Journal of gastroenterology and hepatology》2017,32(3):645-650
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