肝豆状核变性患者血清Ⅲ型前胶原肽测定的临床价值

本文用放射免疫分析法测定了38例HLD患者血清Ⅲ型前胶原肽(PⅢP)的含量,其均值为18.34±12.42ng/ml,较正常对照组7.49±0.81ng/ml明显增高,经青霉胺治疗后的部分轻症患者血清PⅢP水平可恢复正常。认为HLD患者血清PⅢP的检测为反映肝纤维化活动性、程度及肝细胞炎症、坏死的灵敏指标,定期监测对肝硬化的趋势及药物疗效的评估均具有临床价值。     

Recombinant human stem cell factor stimulates growth of a human glioblastoma cell line expressing c-kit protooncogene.

The growth of a panel of eight different human glioblastoma cell lines was examined in a human tumor cloning assay in agar, a tritiated thymidine uptake assay, and by counting cell numbers, in cultures performed in the absence or presence of increasing concentrations (1 to 100 ng/ml) of recombinant human stem cell factor (SCF). Growth of 7 of 8 cell lines was not significantly and reproducibly affected by recombinant human SCF. However, growth of the CRL 1620 cell line could be stimulated up to 5-fold by the cytokine. In contrast to the other cell lines investigated, CRL 1620 expressed the c-kit protooncogene assessed on the mRNA and protein level. Furthermore, SCF-induced proliferation of CRL 1620 cells was sensitive to the tyrosine kinase inhibitor erbstatin. Our data suggest that SCF can be operative in growth modulation of malignant cells outside the hematopoietic system, and this finding should be further studied for its possible clinical implications.     

烧伤大鼠血清对血管内皮细胞通透性的影响

加深入阐明严重烧伤早期全身血管通透性增加继而引直民早期损害的发生机制，本实验应用培养于微孔滤膜上的小牛瞌 及皮细胞单层所构成的选择性通透屏障模型，观察了３０％Ⅲ度烧伤大鼠血清对其影响，结果发现，烧伤早期烧伤血清刺激后ＥＣ单层对Ｈａｎｋｓ液或白蛋白液滤过流量、滤过系数均明显增加。     

医用电子产品的电气安全检测

医用电子技术在不断变化，为提高检测过程的工作效率．医用电子产品的电气安全检测技术也在相应发展．发展的总方向离不开检测综合化和自动化。     

Comparison of fMRI statistical software packages and strategies for analysis of images containing random and stimulus-correlated motion.

The objectives of this study were to use computer-generated phantoms containing real subject motion to: (1) compare the sensitivity of four commonly used fMRI software packages and (2) compare the sensitivity of three statistical analysis strategies with respect to motion correction. The results suggest that all four packages perform similarly in fMRI statistical analysis with SPM2 having slightly higher sensitivity. The most sensitive analysis technique was to perform motion correction and include the realignment parameters as regressors in the general linear model. This approach applies to all four packages examined and can be most beneficial when stimulus-correlated motion is present.     

葡萄籽多酚对多药耐药的逆转作用及其机制

目的：探讨葡萄籽多酚(GSPs)逆转多药耐药的作用及其对多药耐药相关蛋白GSTπ、TopoⅡ α表达的影响。方法：以人乳腺癌MCF-7细胞及其耐药株MCF-7／ADR细胞作为实验对象，用MTT法观察GSPs对多药耐药的逆转作用，免疫细胞化学技术观察GSPs对GSTπ、TopoⅡα等多药耐药相关蛋白表达的影响。结果：GSPs在1．2、2．4mg／L浓度时对细胞无毒性作用，并且均能逆转MCF-7／ADR细胞的多药耐药性，逆转倍数分别为5．77和9．79倍；GSPs可使MCF-7／ADR细胞GSh表达下降，但对TopoⅡ α表达无影响。结论：体外试验证实GSPs具有多药耐药逆转作用，其机制可能与降低肿瘤细胞内GSTπ的表达有关，提示GSPs是一种潜在的肿瘤化疗增敏剂。     

Innervation of the trapezius muscle: is cervical contribution important to its function?

OBJECTIVE: To study the effect of the cervical plexus and the accessory nerve to the function of the trapezius muscle. STUDY DESIGN AND SETTING: Eighteen adult male Sprague-Dawley rats were randomly divided into three groups. The neurotomies were performed in the left sides and the right sides served as within-subject controls. In group A, the accessory nerve was transected. The C2-5 were transected in group B, and both of the accessory nerve and C2-5 were cut in group C. The electrophysiologic, myophysiologic, and histologic changes of the muscles were measured. RESULTS: There were significant differences (P < 0.05) between the three groups in the recovery rates of the transverse area of the muscles. The CMAP recorded from the experimental sides in group B were similar to the control sides. The values of the maximum tension of the tetanus contraction between the two sides showed no differences either (P > 0.05). CONCLUSION: The accessory nerve supplies the most important motor input to the trapezius. Motor innervations of the cervical plexus are not very significant.     

头孢噻肟钠聚合物测定实验条件的改进

目的:对中国药典收载的头孢噻肟钠聚合物测定条件进行改进。 方法 :采用高效液相色谱法 ,以Sephadex G- 10色谱柱 4 2 cm× 1.3cm对 6批样品进行分析 ,分别进样 5 0μl和 2 0 0μl,按外标法计算聚合物的量。结果:头孢噻肟钠在 10～ 6 0 μg/m l范围内线性关系良好 (r=0 .9995 ) ,高中低浓度的加样回收率分别为 10 0 .2 %、10 0 .4 %、99.7% ;RSD分别为 1.7%、1.5 %、1.9% (n =3)。该方法与药典方法比较 ,差异无统计学意义 (P >0 .0 5 )。 结论:本方法简便、快速、可行 ,具有实用价值