德国SIAB-S量表在国内应用的评量标准

目的:分析SIAB-S的评量标准在国内(湖北省宜昌地区)的适用性以及量表各因子间同质性信度分析。方法:采用随机抽样和整群抽样结合方法抽取2004/2005宜昌第14中学、宜昌卫生学校509名学生为调查对象。采用德国SIAB-S量表对抽样对象进行自评筛查和以访谈方式采用DSM-Ⅳ标准进行临床诊断。其中德国SIAB-S量表(1998年第3版),含有87个问题,经归类后主要包括6个方面(因子)的内容:①普通心理病理学和社会整合能力。②体形和苗条理想。③贪食症状。④性欲和体重。⑤相应控制方法、节食和物质滥用。⑥非典型进食发作。询问在最近3个月(目前状态)和最近3个月前(从前)明显形成的症状。对两种方法得出的阳性结果进行对照分析。并使用SSPS11.5统计分析软件对SIAB-S量表进行同质性信度分析。结果:发放问卷509份,收回有效问卷451份,男生75名,女生376名,年龄15～19岁。①德国SIAB-S量表标准:过去发生进食障碍12名;目前存在进食障碍6名;进食障碍总计13名;发病率2.88%。DSM-Ⅳ标准:过去发生进食障碍13名;目前存在进食障碍8名;进食障碍总计16名;发病率3.55%。其中有10名是使用两种方法均能检查出。进行χ2检验,χ2=0.444,P>0.05。差异无显著性。②针对目前诊断进食障碍,该量表的重复度量效果良好(F=897.02,P<0.01)。其6项因子间的标准化信度为0.49,说明该量表的信度还有待进一步的开发完善。对于以前诊断进食障碍,该量表的重复度量效果同样良好(F=547.29,P<0.01)。其6项因子间的标准化信度为0.62,说明该量表的信度较好。结论:SIAB-S量表的德国常模在国内适用,有待进一步完善。     

A novel system for providing compatible blood to patients during surgery: "self-service" electronic blood banking by nursing staff

BACKGROUND: A good blood bank must be able to provide compatible blood units promptly to operating room patients with minimal wastage. A "self- service" by nursing staff blood banking system that is safe, efficient, and well-accepted has been developed. STUDY DESIGN AND METHODS: Specific blood units are no longer assigned to surgical patients who have a negative pretransfusion antibody screen, irrespective of the type of surgery. A computer-generated list of the serial numbers of all group-identical blood units currently in the blood bank inventory is provided for each patient. The units themselves are not labeled with a patient's name. The group O list will be provided for group O patients, the group A list for group A patients, and so forth. Should the patient require transfusion during surgery, the operating room nurses go to the refrigerator, remove any group-identical unit, and check the serial number of the unit against the serial numbers on the patient's list. If the serial number is on that list, the blood bank will accept responsibility for compatibility. The system was implemented in 1995. RESULTS: Since implementation, a total of 2154 patients have undergone operations at this hospital. Thirty-two patients received more than 10 units of red cells each. There were no transfusion errors. The crossmatch-to-transfusion ratio was reduced from 1.67 to 1.12. Turnaround time for supplying additional or urgent units to patients in operating room was shortened from 33 to 2.5 minutes. There was no incidence of a blood unit's serial number not being on the list. Work by nurses and technical staff was reduced by nearly 50 percent. CONCLUSION: The "self-service" (by nursing staff) blood banking system described is safe and efficient. It saves staff time and can be easily set up.     

人脐静脉间充质干细胞原代培养换液频度与细胞增殖生长的关系

目的:体外分离培养人脐静脉内皮及内皮下间充质干细胞,观察采用不同的换液频度与细胞增殖和生长的关系。方法:实验于2006-03/11在辽宁医学院解剖学实验室完成。①选取正常健康产妇顺产或剖宫产的新生儿脐带,由辽宁医学院附属第一医院提供,产妇及其家属均知情同意。②根据更换细胞培养液时间的不同,分为接种后每24h,48h,72h更换培养液组。③无菌条件下将脐带用预热的磷酸盐缓冲液充分洗涤去血渍,从脐静脉一端插入留置针,用预热的磷酸盐缓冲液冲净静脉腔血,止血钳夹闭另一端,采用胶原酶消化法分离人脐静脉内皮及内皮下细胞,取消化30min的细胞悬液进行贴壁培养,以1×108L-1密度接种于24孔培养板中,每孔的细胞数和培养液量相同,按实验设计分组的时间方法更换培养液。④各组自培养24h开始,每天分别取各组细胞4孔,以噻唑蓝比色法测定生长曲线。并采用免疫细胞化学方法对分离的细胞进行表面抗原鉴定。结果:①间充质干细胞的形态学观察:原代培养1周,细胞以梭形细胞为主。传代培养2h细胞开始贴壁变形,24h基本完成贴壁,48h可见部分已贴壁的细胞开始分裂增殖,细胞形态多样,呈椭圆形、梭形、三角形等。5～7d贴壁细胞可见多核的成纤维细胞样。在细胞生长增生过程中,接种后每24h更换培养液组细胞增殖明显,生长状态均最好;接种后每48h更换培养液组次之;接种后每72h更换培养液组细胞增殖较慢,细胞生长状态也较差。②生长曲线:接种后每24h更换培养液组的细胞生长增殖较快,比接种后每48h及72h更换培养液组达到的同样细胞数平均提前2～3d(P<0.05);接种后每48h及72h更换培养液组之间差异无显著性意义(P>0.05)。③间充质干细胞表面抗原特性:免疫细胞化学分析显示CD166呈阳性,vWF呈阴性。结论:胶原酶消化法体外分离获得的人脐静脉内皮及内皮下细胞数量高、活性佳。更换培养液的频度不同,细胞的增殖生长过程和形态变化时间不同,接种后每24h更换培养液更有利于间充质干细胞的增殖生长和纯化。     

Somatosensory and multisensory properties of the medial bank of the ferret rostral suprasylvian sulcus

In ferret cortex, the rostral portion of the suprasylvian sulcus separates primary somatosensory cortex (SI) from the anterior auditory fields. The boundary of the SI extends to this sulcus, but the adjoining medial sulcal bank has been described as “unresponsive.” Given its location between the representations of two different sensory modalities, it seems possible that the medial bank of the rostral suprasylvian sulcus (MRSS) might be multisensory in nature and contains neurons responsive to stimuli not examined by previous studies. The aim of this investigation was to determine if the MRSS contained tactile, auditory and/or multisensory neurons and to evaluate if its anatomical connections were consistent with these properties. The MRSS was found to be primarily responsive to low-threshold cutaneous stimulation, with regions of the head, neck and upper trunk represented somatotopically that were primarily connected with the SI face representation. Unlike the adjoining SI, the MRSS exhibited a different cytoarchitecture, its cutaneous representation was largely bilateral, and it contained a mixture of somatosensory, auditory and multisensory neurons. Despite the presence of multisensory neurons, however, auditory inputs exerted only modest effects on tactile processing in MRSS neurons and showed no influence on the averaged population response. These results identify the MRSS as a distinct, higher order somatosensory region as well as demonstrate that an area containing multisensory neurons may not necessarily exhibit activity indicative of multisensory processing at the population level.     

中药复方治疗黄体功能不全致不孕症170例

0 引言黄体功能不全(LPD)是造成不孕的重要原因之一,约有10%～40%不孕症和反复流产与之有关[1]. 1993/2003我们在临床上以中药复方治疗LPD所致不孕症,疗效满意.     

体液中29种中枢神经系统药物的高效毛细管电泳系统分析方法

建立了高效毛细管电泳系统分析法,分析体液样品中29种CNS药物,作为CNS药物中毒的快速的初筛方法。血、尿样品用有机溶剂萃取,胃液直接进样。用酸、弱碱和中性3组缓冲液体系进行电泳分离。以组分与电渗流迁移时间之比的相对迁移时间和紫外吸收光谱为定性指标。最低检测浓度0.5～5μg·mL^-1。在此法基础上可增加分析品种。通用的提取方法可避免漏检,广谱分离条件便于分类,专一分离条件便于定性。本法用于实际中毒样品分析,证实所建方法操作简便、分析时间短、杂质干扰小。