颈胸滑行皮瓣在单侧面颈部缺损中的应用

目的 :寻求单侧面颈部缺损的即刻修复的有效方法。方法 :采用颈胸滑行皮瓣治疗单侧面颈部缺损。结果 :对发生在腮腺区的恶性肿瘤切除后的大面积缺损的治疗 ,获得了满意效果。结论 :该方法可靠、易行 ,易于被患者接受 ,血供明确 ,成活率高。     

Anti-Tumor Effect and Anti-Inflammatory Activity of Boschniakia rossica

ＢｏｓｃｈｎｉａｋｉａｒｏｓｓｉｃａＦｅｄｔｓｃｈ．ｅｔＦｌｅｒｏｖ（ＢＲ）（1）ｉｓａｈｅｒｂａｃｅｏｕｓｐｌａｎｔｏｆＯｒｏｂａｎｃｈｅｆａｍｉｌｙｇｒｏｗｉｎｇｐａｒａｓｉｔｉｃａｌｌｙｏｎｔｈｅｒｏｏｔＡｌｎｕｓ（Ｂｅｔｕｌａｃｅａｅｆａｍｉｌｙ）ａｔ 1 450 - 1 80 0ｍｅｔｅｒｓａｂｏｖｅｓｅａｌｅｖｅｌｉｎＣｈａｎｇｂａｉＭｏｕｎｔａｉｎ，Ｊｉｌｉｎ，Ｃｈｉｎａ．ＴｈｅｓｔｒｕｃｔｕｒｅｏｆＢＲｃｏｍｐｏｎｅｎｔｈａｓｂｅｅｎｄｅｔｅｒｍｉｎｅｄ（2 ） ａｎｄ 4ｋｉｎｄｓｏｆｉｒｉｄｏｉ…     

Oxymatrine diminishes the side population and inhibits the expression of β-catenin in MCF-7 breast cancer cells

Cancer stem cells (CSCs) play a critical role in both cancer initiation and relapse as they are resistant to most cytotoxic agents and able to proliferate indefinitely. The plant alkaloid oxymatrine has many biological activities including the ability to induce cell cycle arrest and apoptosis, which makes it a potentially useful agent for targeting cancer cells. In order to determine whether it has beneficial pharmacological properties to eradicate CSCs, we analyzed the effects of oxymatrine on MCF-7 breast cancer cells. Cancer stem-like cells’ (side population, SP) identification and sorting were performed. The inhibitory effect of oxymatrine was evaluated on the sorted SP and non-SP cells. The results indicated that oxymatrine caused a dose-dependent reduction in the proliferation of MCF-7 cells and a decrease in SP cells. Wnt/β-catenin signaling pathway was also examined by analyzing the expression of total β-catenin and phosphorylated β-catenin in cytoplasm, and the results showed that the growth inhibitory effects of oxymatrine treatment on MCF-7 cells may be due to the inhibition of SP and Wnt/β-catenin signaling pathway. Further work is warranted to explore whether oxymatrine may be a useful novel therapeutic drug for targeting breast CSCs.

     

Repair effect of Schwann cells modified by microgene pSVPoMcat on injured spinal cord in rats

OBJECTIVE: To observe the repair effect of Schwann cells (SCs) modified by microgene pSVPoMcat on injured spinal cord in rats. METHODS: Semi-transection injury at the level of T(8) of spinal cord was made with cutting method on 120 Sprague Dawley (SD) rats. Then 40 rats implanted with SCs modified by microgene pSVPoMcat were taken as Group A, 40 rats implanted with simple SCs as Group B and the other 40 rats were taken as the control group (Group C). The functional recovery of the rats was observed through combined behavioral score (CBS) and cortical somatosensory evoked potential (CSEP), and the expression of the glial fibrillary acidic protein (GFAP) was measured with in situ hybridization and immunocytochemistry. At 3 months after operation, the rats were examined with magnetic resonance image (MRI), and the neurofilaments (NF) of the axons were stained with immunohistochemical method. RESULTS: GFAP expression in Group A was significantly lower than that of the other 2 groups. MRI showed that the spinal signals in the injured area recovered fundamentally in Group A, didn't recover in Group B and malacia focus was found in Group C, which was same as the results of NF staining. Wave amplitudes in incubation periods in Group A and Group B tended to recover. It recovered to the normal level in Group A, which was similar to the results of CBS. CONCLUSIONS: SCs modified by microgene pSVPoMcat can inhibit GFAP expression, improve the growth of the axons and the functional recovery of neurons after spinal cord injury.     

万年干支和针灸五种按时取穴推算盘

文章介绍了以表、盘合二为一形式设计的推算盘，可以推算年月日时干支和子午流注的5种开穴法，具有直观、易学、快捷、准确，内容丰富，永久使用等特点，是中医、武林、天文、历法、气象、历史等工作者和人们日常生活中推算干支，以及针灸按时选穴的理想工具。     

去分化与细胞组织损伤再生

去分化是发育生物学和形态学领域一个古老又常新的现象。既往研究多注重去分化和肿瘤发生的关系。我们的系列研究发现去分化和细胞组织损伤再生有着重要和必然的联系。在周围神经损伤再生中发现局部有大量施万细胞通过自噬机制吞噬消化自身髓鞘成分和衰老的细胞器，同时以胞质脱落的方式芽生出部分胞质脱落体，细胞内含大量游离核糖体和多聚核糖体等原始细胞器，保留极少量有活力的线粒体和高尔基体，呈现去分化后的幼稚化状态。在长春新碱诱导L02(肝细胞株)细胞损伤实验中发现L02细胞中有大量的自噬体，细胞周围也出现大量发芽脱落后游离于细胞外的胞质脱落体．细胞耐过之后呈现幼稚化．我们也称之为去分化。细胞经过去分化减轻代谢负担，清除衰老和受损的细胞器，恢复活力．接着大量增殖以修复重建损伤组织。在当前大部分科研人员一致看好干细胞在组织损伤再生中的重要作用的背景下，本研究根据长期观察实践．提出成熟细胞去分化幼稚化参与再生重建的观点，以供同行专家讨论。     

新型强心药物──Pimobendan的合成研究

本文叙述了以乙酰苯胺为起始原料合成新型强心药物Ｐｉｍｏｂｅｎｄａｎ的新的合成方法，总收率为３．５１％，关键中间体及最终化合物的结构经光谱分析得到了确证。     

延边地区朝鲜族正常足月新生儿的身长、体重及头径线的研究

研究了延边地区２１１例朝鲜族足月新生儿生理常数，建立了朝鲜族新生儿的头径线、体重、身长的正常数值，并得出三者之间一致性增减的相互关系，即体重较大的新生儿身长也高、头径也较长．     

内皮细胞性一氧化氮合酶基因多态性与缺血性脑卒中的相关性研究

目的　探讨大连地区汉族人内皮细胞性一氧化氮合酶 (ecNOS)基因多态性与缺血性脑卒中的关系。方法　应用聚合酶链反应对 170例缺血性脑卒中群体及 90例非脑血管病群体ecNOS基因第 4号内含子多态性进行分析。结果　在缺血性脑卒中组中 ,高血压、糖尿病、吸烟、纤维蛋白原、颈内动脉狭窄与对照组比较 ,有显著性差异 (均P <0 .0 5 )。非条件性Logistic回归分析证实 ,ecNOS基因多态性不是缺血性脑卒中的独立危险因素。结论　ecNOS 4a/b基因多态性与大连地区汉族人缺血性脑卒中无相关性     

显微手术治疗大中型听神经瘤

目的　总结采用显微外科技术治疗听神经瘤的体会 ,提高面神经保留率 ,预防并发症。方法　对我科近 2年来经显微手术治疗的 10例听神经瘤进行回顾性分析、研究。结果　肿瘤全切除率为 90 % ,面神经保留 9例(90 % ) ,耳蜗神经保留率为 30 % ,无手术死亡。结论　显微外科手术是治疗大、中型听神经瘤的有效方法 ,熟悉肿瘤与面神经的病理解剖关系 ,有助于提高手术效果。