肝细胞癌发生中丙型肝炎病毒NS3蛋白对诱导型一氧化氮合酶表达的影响

OBJECTIVE: To investigate hepatocarcinogenesis by detecting the effect of HCV NS3 protein on expression of inducible nitric oxide synthase (iNOS) mRNA in hepatocellular carcinoma (HCC) and pericarcinomatous liver tissues(PCLT). METHODS: The expression of HCV NS3 protein and iNOSmRNA was detected by immunohistochemical technique (SP method) and in situ hybridization in specimens of HCC and PCLT from 52 patients with HBV(-). The relationship between the positive rate and the signal strength was tested statistically. RESULTS: The positive rate of HCV NS3 protein in HCC was lower (46.2% vs. 67.3%), and signal strength was weaker than those in PCLT. The signal strength of HCV NS3 protein was correlated with the degree of carcinomatous cells differentiation in HCC (P < 0.01). The positive rates (80.8% and 90.4%) of iNOSmRNA in HCC and PCLT had no statistical difference. Signal intensity of iNOSmRNA in HCC was weaker than that in PCLT. The higher HCVNS3 protein expression level was, the stronger iNOSmRNA signals in PCLT (P < 0.01). In particular, the localization and patterns of HCV NS3 protein were much similar to those of iNOSmRNA in the PCLT of some patients. CONCLUSIONS: HCV NS3 protein may exert its hepatocarcinogenic effect in the early stage on host cells by increasing product of nitric oxide, which may bring about transformation of hepatocytes.     

空回肠出血诊断分析

目的：探讨空回肠出血的病因及诊断方法的选择。方法：33例空回肠出血患者分别行胃镜、结肠镜、全消化道钡餐、核素扫描、血管造影、剖腹探查及术中肠镜等单项或多项检查。手术及病检证实。结果：33例患者中,小肠肿瘤占13例（39.39%),以平滑肌瘤多见,小肠感染性疾病8例（24.24%),Meckel‘s憩室7例（21.21%),血管病变（包括血管瘤）3例（9.1%),其它2例（6.06%),其临床表现为不同程度的消化道出血,黑便多见(42.42%),约1/3的患者伴有腹痛,以中度贫血为多见(48.48%).核素扫描检查7例,5例均发现小肠出血部位.经手术和病理检查证实均为Meckel‘s憩室;胃镜和/或结肠镜检查20例,5例提示小肠病变;剖腹探查6例,其中1例蓝色橡皮疱痣血管瘤、1例血管畸形、1例坏死性小肠炎,3例经术中肠镜发现1例副伤寒、1例空肠平滑肌瘤、1例肠道钩虫病。结论：空回肠出血以肿瘤为最常见病因,对不明原因的中度贫血伴黑便者应高度怀疑小肠病变,核素检查对诊断Meckel‘s憩室阳性率高。     

Externalization and internalization of cardiac endothelin receptors during different phases of sepsis in rat.

Objective. To study the redistribution ofendothelin- 1 (ET- 1 ) receptors in two subcellular organelles, the sarcolemreal membrane and the light vesicle, of rat heart during the progression of sepsis. Methods. Sepsis was induced by cecal ligation and puncture (CLP). ET1 receptor was assayed by using[125I]-ET1 binding. Marker enzyme activities, protein yield, and dry-to-wet weight ratio of cardiac membraneswere measured. Results. Septic rat heart exhibited two distinct phases: an initial hyperdynamic phase( 9h after CLP; early stage of sepsis) followed by a hypodynamic ( 18h after CLP, late stage of sepsis) phase. [125I]-ET1 binding study showed that during early stage of sepsis, the Bmax of ET1 receptors was increased by 30% in sarcolemma but decreased by 19% in light vesicles, while during late stage of sepsis, the Bmax was decreased by 24% in sarcolem-ma but increased by 38% in light vesicles. The total binding of sarcolemma and light vesicles was increased by 25% during early stage of sepsis but decreased by 17% during late stage of sepsis. Conclusions. These data indicated that ET1 receptors in the rat heart were externalized from light vesicles to sarcolemmal membranes during early hyperdynamic phase while internalized from surface membranes to intracellu-lar compartment during late hypodynamic phase of sepsis.     

RADIATION INDUCED PROGRESSIVE DECREASING IN THE EXPRESSION OF REVERSE TRANSCRIPTASE GENE OF hEST2 AND TELOMERASE ACTIVITY

Objectites. In order to identify the relationship between telomerase and the biological effect of radiation injury, and investigate the role of human telomerase catalytic subunit gene (hEST2) reverse tranacriptase(RT) seg-ment in the expression of telomerase activity. Methods. Tumor FIeLa cells, KB cells and A431 cells were employed to measure the change in telomeraseactivity after 60Co-ray irradiation at RNA level and protein level. Quantitative PCR and Northern blotting wereused to determine the expression of bEST2 RT segment that encodes seven motifs of the human telomeres, a PCR-besed telomeric repeat amplification protocol (TRAP)was used to assay telomerase activity after exposure toradiation. Results. Both of telomerase activity and the expression hEST2 RT segment were decreased with increasingdosage of radiation. In addition, testing the expression of motifs domain is similar to the measurement of telomerase activity. Conclusion. The detection of the hEST2 BT segment by Northern blotting and quantitative PCR are new methods for testing Uflomerase activity. Furthermore, radiation can cause a dose-dependent decrease in telomerase activity. The effect of radiation on telomerase is one possible reason for the death of cancer ceils after irradiation.     

经支气管针吸活检的作用

目的 探讨和评价经支气管针吸活检术的作用和临床意义。方法 在纤维镜室对259例肺癌患者行常规纤支镜检查,对其中145例合并有纵隔及肺门淋巴结肿大或纵隔肿物患者行TBNA。结果 259例患者中的202例经纤支镜检查获得阳性结果。其中常规纤支镜检查确诊143例,诊断阳性率为55.2%,59例患者单独由TBNA检查确诊,使纤支镜检查的诊断阳性率由55.2%上升至77.9%。结论 ＴＢＮＡ可作为纤支镜检查的常规项目开展,可明显提高纤支镜的诊断阳性率,对纵隔疾病的诊断和肺癌的临床分期有着独特的作用。     

侧脑室肿瘤手术切除15例的临床疗效

目的 :探索手术切除侧脑室肿瘤的方法。方法 :15例侧脑室肿瘤分别为室管膜瘤、脑膜瘤、室管膜下瘤、胶样囊肿、胶质细胞瘤、脉络丛乳头状瘤及脑血管畸形 ,其中 7例侧脑室额角肿瘤经额中回皮质入路 ,2例侧脑室体部肿瘤经胼胝体前部入路 ,5例侧脑室三角区肿瘤经顶枕部皮质入路 ,1例侧脑室颞角肿瘤经颞叶皮质入路。结果 :除 1例侧脑室额角胶质细胞瘤次全切除术后行放疗和化疗外 ,其余肿瘤均手术全切 ,所有病人术后随访 6个月至 1年 ,术后恢复良好 ,参加日常工作。结论 :侧脑室肿瘤绝大多数可行全切除且预后良好     

湿温病湿重于热证的动物实验研究

用多因素综合造模方法,模拟湿温病湿重于热证的发生发展过程,观察实验动物血液生化、免疫功能的变化及病理切片。并用自拟中药燥湿运脾汤治疗模型动物,观察其治疗前后的变化。初步揭示湿重于热证的病理实质以及燥湿运脾汤的作用机理。     

辅以钙剂治疗急性腹泻52例临床疗效观察

目的：探讨在常规综合治疗基础上辅以钙剂治疗急性腹泻的疗效。方法：采用随机单盲平行对照方式,１０４例急性腹泻患者按病后诊治顺序随机分为两组,对照组５２例给常规综合治疗,治疗组５２例在常规综合治疗基础上加用钙剂治疗３天（１０％葡萄糖酸钙针剂２０ｍｌ,缓慢ｉｖ,ｑｄ）。结果：经ｘ＾２检验,两者疗效有显著性差异（Ｐ〈０．０５）。结论：在常规综合治疗基础上钙剂治疗急性腹泻可提高疗效。     

通络活血汤治疗结节性红斑的临床观察

目的：观察通络活血汤对结节性红斑的治疗作用。方法：对确诊的结节性红斑患者给予通络活血汤治疗,每日一剂,水煎服,疗程 １个月。结果：４９例结节性红斑患者治愈 ３６例,有效 １３例,复发 ３例,总有效率 １００％,复发率 ６．１２％,其治愈率（７３．４６％）同对照组比较有显著性差异（Ｐ＜０．０１）。结论：通络活血汤治疗结节性红斑疗效高、复发率低、无副作用,与西药相比,更适合于临床治疗。     

两种不同血管紧张素ⅡⅠ型受体拮抗剂治疗轻中度原发性高血压的临床研究

目的:探讨缬沙坦和氯沙坦治疗轻中度原发性高血压的临床疗效及安全性。方法:117例轻中度原发性高血压患者随机分为两组,缬沙坦(治疗)组59例,80～160mg/d,口服,疗程半年;氯沙坦(对照)组58例,50～100mg/d,口服,疗程半年。治疗前后测偶测血压、动态血压、肝肾功能、血脂、血糖、血尿酸,血浆肾素活性(PRA)、血管紧张素Ⅱ(AngⅡ)醛固酮(ALd)、内皮素(ET)等,并观察心脑事件发生情况。结果:治疗组总有效率74.6％(44/59例),降压幅度26.2/15.1mmHg,谷/峰(T/P)比值:收缩压(SBP)0.28、舒张压(DBP)0.78,与对照组总有效率74.1％(43/58例),降压幅度25.9/15.8mmHg,T/P比值:SBP 0.81、DBP 0.79均相似(P均>0.05)。两组治疗后PRA、AngⅡ均升高(P均<0.05);Ald、ET均降低(P均<0.05),但组间对照无显著性差异(P>0.05)。血尿酸也明显下降(P均<0.05),且基础值越高,降幅越大,对于治疗前高尿酸血症患者,对照组降压大于治疗组(P<0.05)。半年随访,两组心脑血管事件发生率相似(P>0.05),治疗组不良么应轻微。结论:缬沙坦是一种安全、作用持久、稳定、耐受性好、用药方便的治疗轻中度原发性高血压的新型AngⅡⅠ型受(AT1)拮抗药物之一,值得临床推广应用。