Messenger-rnas for activin and activin receptors are expressed in fetal bovine heart endothelial-cells

Inhibin and activin, members of transforming growth factor beta (TGF beta) superfamily, have been localized immunohistochemically in fetal bovine heart endothelial cells (FBHE), suggesting that these cells produce inhibin/activin proteins. In addition, the mRNAs coding for alpha-, and beta A-subunits of inhibin/activin, and activin receptor II were expressed in these cells by in situ hybridization technique. It is concluded that inhibin/activin and activin receptor are expressed in FBHE and activin may have autocrine functions in these cells.     

微波治疗耳鼻喉科疾病疗效观察

探索耳鼻喉慢性炎症的简便有效疗法。方法：对１０２例病人表麻下用微波辐射探头定量向病 变组织输入电磁波。结果：随访观察半年以上,治愈率达８８．２４％,有效率达１００％。结论：此方法操作简便,疗 效好,病人痛苦小,值得临床推广。     

咬合板治疗颞下颌关节紊乱病的时机与疗效

目的：通过对颞下颌关节紊乱病（ＴＭＤ） 的临床表现及咬合板治疗的疗效进行分析,探讨ＴＭＤ 的最佳治疗时机。方法：对１５６ 例经咬合板治疗的ＴＭＤ患者就诊时间及治疗２ 个月后治疗效果做分类统计分析。结果：治疗２ 个月后疼痛、弹响及张口受限的总治愈率分别为８１．４％ 、４９．０％ 和８１．５％ 。其中于发病４ 周内就诊的患者治愈率分别为９３．２％ 、７７．３％ 和９３．２％ ,于４ 周后就诊的患者治愈率分别为７３．９％ 、４０．８ ％ 和３０．０ ％ ,两组差异显著（ 弹响及张口受限, Ｐ＜ ０．０１,疼痛Ｐ＜０．０５）。结论：ＴＭＤ在发病４ 周内进行咬合板治疗效果满意。     

聚合酶链反应检测细菌16S rRNA基因

根据细菌１６ＳｒＲＮＡ基因的高度保守性,设计合成所有细菌、革兰氏阳性细菌及革兰氏阴性细菌的共同引物,采用聚合酶链反应检测已知细菌１３株,三对引物分别扩增的阳性率为１００％,倍比稀释法能检出细菌的最低浓度为４ＣＦＵ·ｍｌ－１,同时检测临床样本４０份,阳性率为６７５％（２７／４０）,同期细菌培养阳性率为４５％（１８／４０）,二者比较差异有显著性（Ｐ＜０．０５）。结果提示聚合酶链反应检测细菌１６ＳｒＲＮＡ基因具有高度的特异性和敏感性。     

Development and clinical application of dental spot weld & heat-treat tri-uses device]

A spot weld & heating treatment tri-uses device based on modern mechanical-electric technique, was developed for dental spot weld, stainless steel orthodontic wire heating-reversion and Ni-Ti orthodontic wire heating-overload metamorphism. The device will be very helpful instrument in orthodontic clinic. The paper not only described the operating methods of the device, but also discussed the Performance-improved principles of stainless steel orthodontic wire and the principles of heating overload metamorphism of Ni-Ti orthodontic wire.     

Predictors of an acute antidepressant response to fluoxetine and sertraline.

Sertraline and fluoxetine have different pharmacologic and pharmacokinetic profiles which may be of clinical relevance in the determination of response in different subtypes of depression. A randomized, double-blind, 6-week study comparing sertraline (50-100 mg/day) with fluoxetine (20-40 mg/day) in 286 outpatients with major depression, who had demonstrated comparable efficacy and tolerability for the two drugs, was analysed by subgroups of patients at baseline with melancholia, severe depression, single depressive episode, multiple depressive episodes, high anxiety, low anxiety, psychomotor retardation and psychomotor agitation. Multiple logistic regression with regressors including treatment-by-subgroup variables revealed that, within certain subgroups, the efficacy might differ substantially from that of the whole treatment group. However, the only treatment-by-subgroup interaction term that was significant was anxiety (P < 0.05). There was no evidence of interaction in single or recurrent episode subgroups, and these were not included in subsequent analyses. Subsequent two-sample statistical comparison tests of response (i.e. Hamilton Depression Scale reduction > or = 50%) rates at study endpoint between treatment groups demonstrated that patients with melancholic depression and those with symptoms of psychomotor agitation yielded a significantly greater proportion of responders with sertraline compared to fluoxetine (P < 0.05). Response rates in sertraline- and fluoxetine-treated patients, respectively, were: overall study 59%, 51%; melancholia 59%, 44%; severe depression 59%, 41%; low anxiety 71%, 55%; high anxiety 47%, 48%; psychomotor retardation, 48%, 46%; and psychomotor agitation 62%, 39%. Multiple logistic regression adjusting for possible confounding factors, that included a treatment by anxiety interaction term, also led to similar findings. In particular, the analysis showed that significant differences existed in favour of sertraline in patients with low anxiety in the melancholia and severe depression subgroups (P < 0.05), indicating that these characteristics predicted a superior response to 6 weeks of treatment with sertraline relative to fluoxetine. Sertraline also demonstrated advantages over fluoxetine on parameters such as sleep and weight disturbance in severely depressed patients, and sleep disturbance, weight, cognitive disturbance and retardation in melancholic patients.     

药流与负压吸宫法的效果和可接受性研究

研究的目的:比较RU486/Cytotec药物和负压吸宫术两种流产方法的效果,以及医学的和个人的可接受性.对象为通过咨询,介绍两种流产方法后,让对象自愿选择而组成.年龄在20～34岁.药物组100例,闭经35～42天,第1天口服RU486 600mg,第3天服Cytotec(PGE1)0.4mg,第17、43天回医院随访.手术组100例,闭经≤56天,负压吸宫术后第14、43天回医院随访.结果:完全流产率药物组为89%,手术组为100%.对象选择这两种流产方法的主要原因:药物组94%的人认为痛苦少,手术组的55%认为手术快、节省时间,而且手术同时可取出或放置宫内节育器(占45%).结论:RU486/Cytotec药物流产和负压吸宫术在各自适合的人群中都具有高度的可接受性.两种方法各具优缺点,不能相互取代,二者相辅相成,取长补短,将使终止妊娠的措施更为安全     

Influence of grass pollen immunotherapy on cellular infiltration and cytokine mRNA expression during allergen-induced late-phase cutaneous responses.

We have studied the influence of grass pollen immunotherapy on cellular infiltration and cytokine mRNA expression during allergen-induced late-phase cutaneous responses. In a double-blind, placebo-controlled trial of immunotherapy in 40 adult hay fever sufferers, clinical improvement was accompanied by a decrease in the size of the late-phase skin response. When the immunotherapy-treated group was compared with the placebo group, analysis of skin biopsies obtained 24 h after intradermal allergen revealed a significant reduction in the number of infiltrating CD3+ (P = 0.04) and CD4+ (P = 0.009) cells and a trend for a decrease in EG2+ eosinophils (P = 0.08). Treatment did not influence allergen-induced recruitment of CD8+ cells, neutrophils, or macrophages. Unexpected increases in expression of CD25 (P = 0.006) and HLA-DR (P = 0.007) were observed in the actively treated group. In situ hybridization using a panel of riboprobes demonstrated "TH2-type" (IL-4, IL-5) cytokine mRNA responses in both groups of patients. In contrast, significant hybridization for IL-2 (8/16 patients, P = 0.02) and for interferon-gamma (6/16 patients, P = 0.04) was observed only in the actively treated group. These findings indicate that immunotherapy is associated with suppression of allergen-induced CD4+ T lymphocyte infiltration, but among the cells that are recruited, there is upregulation of CD25 and HLA-DR. At least in this model, immunotherapy does not appear to affect expression of TH2-pattern cytokines in response to allergen exposure, but expression of mRNA for Th1-type cytokines was enhanced in half of the patients. The results support the view that immunotherapy may possibly be working through induction of T cell tolerance.     

电针对去势大鼠外周血淋巴细胞雌激素受体影响的研究

目的　从生殖内分泌 -免疫网络角度探讨电针治疗围绝经期综合征的作用机制。方法　以去势大鼠为实验对象 ,取双侧三阴交、太溪、后三里穴 ,采用放射免疫法和流式细胞分析技术 ,观察电针治疗对去势大鼠血清雌激素 (E2 )、促黄体生成素 (LH)、促卵泡生成素(FSH)及外周血淋巴细胞内雌激素受体 (ER)水平的影响。结果　电针可使去势大鼠血清E2 明显升高 ,LH及FSH明显下降 ,外周血淋巴细胞内ER明显升高 ,与造模组比较有显著性差异。结论　电针可明显改善去势大鼠的生殖内分泌功能 ,并能调节免疫细胞的雌激素受体表达。