联苯双酯对鼠肝细胞的生化药理作用

本文结果表明,联苯双酯可增加肝细胞膜流动性。同时,联苯双酯具有保护线粒体结构和功能的作用:消除表面活性剂Triton X-100引起的线粒体肿胀;增加线粒体呼吸控制比;在氧化反应基础上,保持偶联磷酸化,增加线粒体ATP合成效率;促进线粒体对Ca²⁺的摄取。     

A model for health: its use in an undergraduate nursing programme

A great deal has been said about the need for health professionals to move away from a disease model of care and place more emphasis on health and its promotion and maintenance. One way of introducing a health approach to the service is to emphasise it in the basic education of nurses and doctors.The curriculum for the BA Nursing Studies programme at Glasgow College of Technology is based on a health model for nursing, a model based on activities of living. Initially the emphasis is on health and then the move is towards the study of dysfunction and disease processes. However, a strand of health education is retained throughout the programme, and students are encouraged to identify opportunities for health teaching in both hospital and community settings. The $\text{4}\text{1}\text{3}\text{-}\text{year}$ nursing programme gives a framework for a health approach which looks towards the year 2000.     

Depletion of myocardial glutathione: its effects on heart function and metabolism during ischaemia and reperfusion

To determine the importance of intracellular reactive oxygen metabolites in ischaemia and reperfusion, rat hearts were depleted of glutathione, a major intracellular antioxidant, prior to total global or low flow ischaemia. Chronic pretreatment of rats with up to 7 mmol.kg-1 L-buthionine-S,R-sulphoximine over a period of 96 hours resulted in 85% depletion of total myocardial glutathione. Subsequent recovery of glutathione after treatment with the sulphoximine was slow, reaching only 54% of control levels, 96 hours after the final dose. Depletion of myocardial glutathione by 70% did not increase the metabolic consequences of either total global or low flow ischaemia in the isolated perfused heart, as determined by 31P NMR spectroscopy and high performance liquid chromatographic analysis of purine release. During low flow ischaemia glutathione depletion caused a significant reduction in purine release. On reperfusion, functional recovery was depressed compared to controls. Hearts depleted of glutathione also showed a decrease in developed tension during normoxic perfusion prior to low flow ischaemia. There was no difference between the two groups before or after total global ischaemia. These results do not support the hypothesis of free radical mediated reperfusion injury; however, the method described here for the depletion of glutathione should prove a useful tool for further investigation into the role of glutathione in cardiac metabolism and function.     

Acute myocardial ischemia: MR imaging with Mn-TP

Cardiac-gated magnetic resonance (MR) imaging was performed in rats to determine the effects of manganese ethylenediaminetetraphosphonate (TP). Ten normal rats received Mn-TP in a dose of 50 mumol/kg through a tail-vein injection. Spin-echo MR images were obtained before and every 10 minutes after Mn-TP injection for 1 hour. Cardiac signal intensity (SI) increased more than 70% after Mn-TP injection and remained nearly unchanged 1 hour after injection. Myocardial T1 was 517 +/- 49 msec in eight control rats and 282 +/- 61 msec (P less than .001) in six rats 81 +/- 0 minutes after injection. Nine rats underwent occlusion of the left anterior descending coronary artery prior to MR imaging. Images were obtained before and 15, 30, and 60 minutes after Mn-TP injection. In normal myocardium, SI increased up to 82% and remained elevated for 1 hour. In ischemic myocardium, SI rose 11%, leading to a marked contrast between the two tissue zones. T1 was also different in the two regions: In normal tissue, it was 206 msec +/- 54; in ischemic tissue, 338 +/- 82 (P less than .001). With T1-weighted MR imaging, Mn-TP showed a potential for delineating the jeopardized area after acute myocardial ischemia.