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101.
We describe a highly efficient stable gene transfection procedure for Chinese hamster ovary (CHO) cells using a modification of the calcium phosphate-DNA coprecipitation method. We have found that treatment of CHO cells with chloroquine increases the efficiency of gene transfer by up to 20-fold (from approx. 0.01% to approx. 0.2%) when transfection is done using the pSV2-neo plasmid. The optimized transfection procedure requires that CHO cells to be incubated with calcium phosphate-DNA coprecipitate and chloroquine (100 µM) for a total of 16 h. By using high-molecular-weight human genomic DNA as a DNA source for transfection, we show that this procedure is equally efficient for stably transferring a much larger gene, such as the 49-kb human hypoxanthine phosphoribosyltransferase gene. 相似文献
102.
103.
类黄酮物质抗动脉粥样硬化作用研究进展 总被引:9,自引:0,他引:9
人类植物性膳食成分中存在丰富的类黄酮物质,具有多种生物活性。近几年的流行病学研究和试验研究显示,类黄酮物质可以抑制动脉粥样硬化的发生与发展,从而降低心脑血管疾病的发生率和死亡率。本就类黄酮物质抗动脉粥样硬化的作用机制作一综述。 相似文献
104.
Brain neurons and glial cells express Neu differentiation factor/heregulin: a survival factor for astrocytes. 总被引:8,自引:1,他引:7 下载免费PDF全文
R Pinkas-Kramarski R Eilam O Spiegler S Lavi N Liu D Chang D Wen M Schwartz Y Yarden 《Proceedings of the National Academy of Sciences of the United States of America》1994,91(20):9387-9391
Neu differentiation factor (NDF, also called heregulin) was isolated from mesenchymal cells on the basis of its ability to elevate phosphorylation of ErbB proteins. Earlier in situ hybridization analysis showed that NDF was transcribed predominantly in the central nervous system during embryonic development. To gain insights into the role of NDF in brain we analyzed its distribution by immunohistochemistry and in situ hybridization. Late-gestation (day 17) rat embryos displayed high NDF immunoreactivity in both motor (e.g., putamen) and limbic (e.g., septum) regions. Lower levels of the factor were exhibited by adult brain, except for the cerebellum, where NDF expression was increased postnatally. Both neurons and glial cells were identified by immunohistochemistry as NDF-producing cells (e.g., pyramidal neurons in the cerebral cortex and glial cells in the corpus callosum). By establishment of primary cultures of rat brain cells we confirmed that NDF was expressed in neurons as well as in astrocytes. In addition, by using such primary cultures we observed that NDF treatment exerted only a limited mitogenic effect, which was accompanied by significant acceleration of astrocyte maturation. Furthermore, long-term incubation with the factor specifically protected astrocytes from apoptosis, implying that NDF functions in brain as a survival and maturation factor for astrocytes. 相似文献
105.
Fusiform posterior cerebral artery aneurysm treated with excision and end-to-end anastomosis. Case report 总被引:2,自引:0,他引:2
A case of a ruptured fusiform aneurysm of the posterior cerebral artery is reported. The aneurysm was excised and end-to-end anastomosis was carried out between the two ends of the posterior cerebral artery. There is no previous report of a posterior cerebral artery aneurysm treated with this technique. The pertinent literature is reviewed and the significance of this technique in the treatment of unclippable cerebral aneurysms is discussed. 相似文献
106.
慢性乙肝患者血清细胞因子、乙肝病毒与肝纤维化的关系 总被引:1,自引:0,他引:1
目的:探讨慢性乙肝患血清细胞因子(TGF-β,TNF-α,IL-6,IL-8),乙肝病毒(HBV)对肝纤维化(HA,PCⅢ,C-Ⅳ,LN)的影响作用。方法:采用ELISA和RIA方法检测171例慢性乙肝患血清TGF-β,TNF-α,IL-6,IL-8,HLA,PCǚ,C-Ⅳ,LN的水平,HBV-DNA定量检测采用PCR方法。结果:慢性乙肝患血清TGF-β,TNF-α,IL-6,IL-8,HA,PCⅢ,C-Ⅳ,LN的含量均不同程度高于对照组,且随肝损害程度的加重而升高,与肝损害程度呈正相关关系(P<0.05或P<0.01);须血清TGF-β1水平与血清HA,PCⅢ,C-Ⅳ,LN水平具有直线相关关系。HBV-DNA阳性组(Ⅱ组)均高于HBV-DNA阴性组(I组),且在慢性中,重度肝炎间差异显(P<0.05)。结论:血清TGF-β,TNF-α,IL-6,IL-8参与慢性乙肝的发病机制及肝纤维化的形成,且与病毒复制的活跃程度相关。 相似文献
107.
108.
经直肠超声引导前列腺穿刺活检203例临床分析 总被引:8,自引:1,他引:7
目的评估经直肠超声引导的前列腺六针穿刺活检在前列腺癌及前列腺其他疾病的诊断和鉴别诊断的价值。方法对指肛检查阳性,血清PSA〉4pg/L及经直肠超声检查前列腺声像图异常怀疑有占位性病变的203人进行经直肠超声引导的前列腺穿刺活检。结果穿刺活检的203例病理结果:良性前列腺增生(BPH)104例占51.24%,前列腺癌(PCa)95例占46.80%,前列腺结核及前列腺平滑肌肉瘤各2例,分别占0.98%。结论经直肠超声引导的前列腺穿刺活检其操作简单,病人痛苦小,并发症少,较安全。在前列腺癌及其他前列腺疾病的诊断与鉴别诊断中有重要的临床价值。 相似文献
109.
目的:了解烧伤病区铜绿假单胞菌(Pseudomonas aeruginosa,PA)感染的分子流行病学特征。方法:采用脉冲场凝胶电泳(PFGE)分型的方法,对15株分离自烧伤临床病人的多重耐药PA染色体组DNA进行分析。结果:15株检测菌株中有9株菌分别属于两种不同的PFGE图谱类型,具有同源性的菌株占测试菌株的60%。结论:导致我院烧伤患者感染多重耐药的PA中有同源性的比例较高,提示在烧伤救治中采取有针对性的措施,防止烧伤病房内的交叉感染对于降低烧伤感染率、提高烧伤救治水平具有重要意义。 相似文献
110.
The significance of a rapid cold hemagglutination test for detecting mycoplasma infections in children with asthma exacerbation. 总被引:5,自引:0,他引:5
Ya Ting Chang Yao Hsu Yang Bor Luen Chiang 《Journal of microbiology, immunology, and infection》2006,39(1):28-32
BACKGROUND AND PURPOSE: Mycoplasma pneumoniae infection is a frequent cause of community-acquired respiratory infections in children and adults. However, standardized, rapid, specific methods for its diagnosis are lacking. The relationship between M. pneumoniae infection and asthma exacerbation has been recently discussed in the literature. We investigated the accuracy of rapid detection of mycoplasma infection by cold hemagglutination test compared to conventional enzyme immunoassays. The clinical characteristics of mycoplasma infection seen during emergent visits in asthmatic children were reviewed. METHODS: We retrospectively reviewed medical records of patients with asthma exacerbation visiting the Department of Pediatric Emergency, National Taiwan University Hospital, over a 12-month period. Subjects 2-18 years of age diagnosed with asthma at our outpatient clinic were included in this study. Patients with immunodeficiency, congenital anomalies, neurological diseases and irregular follow-up were excluded. RESULTS: A total of 269 children (174 males and 95 females) with a mean (+/- standard deviation) age of 6.15 +/- 3.08 years were included. The prevalence of asthma exacerbation in regular follow-up patients was 13.4%, and as many as 19.6% of cases (74/378 person-times) required hospitalization. Asthma attacks were most prevalent during December. 126 patients had both rapid cold hemagglutination testing and mycoplasma immunoglobulin M titers determined using acute blood samples drawn in the emergency room; 46 (36.5%) of these patients demonstrated mycoplasma infection. Sensitivity and specificity of the rapid cold hemagglutination test was 78.3% and 41.3%, respectively. The positive predictive value was 43.4%. Comparison of patients with or without mycoplasma infection revealed no differences in gender, age, chest X-ray findings, and most symptoms/signs and laboratory data, except that more signs of fever and auscultatory rales were seen in the non-mycoplasma infection group. CONCLUSIONS: Mycoplasma infections could be an exacerbating factor for asthma, and the rapid cold hemagglutination test should not be a guideline for prescribing macrolides in the emergency room. 相似文献