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81.
链球菌蛋白G应用于ELISA的实验研究 总被引:2,自引:0,他引:2
对酶标记链球菌蛋白G(SPG)在ELISA的应用条件进行了研究并与SPA作了系统比较。SPG与IgG结合受温度影响,37℃结合活性高于室温和4℃;反应时间定为2h反应溶液pH值升高,SPG的结合力亦增强。在同等条件下与酶标记SPA进行比较,结果表明SPG对小鼠单克隆抗体、羊抗人、兔抗IFN-γ和驴抗兔Igg以及牛、大鼠和人IgG的结合力均明显高于SPA。SPG用于大鼠血清抗IFN-γ抗体的检测,其阳性率和抗体滴度均大大高于SPA。因此,SPG作为一种广谱的、高亲和力的IgG结合物,是应用于ELISA的理想的替代二抗。 相似文献
82.
The prevalence of LRRK2 Gly2385Arg variant in Chinese Han population with Parkinson's disease. 总被引:2,自引:0,他引:2
Cao Li Zhang Ting Xiao Qin Wang Ying Bai Li Lu Guo Qiang Ma Jian Fang Zhang Jing Ding Jian Qing Chen Sheng Di 《Movement disorders》2007,22(16):2439-2443
We conducted a case-control study to determine the prevalence of the LRRK2 Gly2385Arg variant in patients with Parkinson's disease in Han population in mainland China. Heterozygous LRRK2 Gly2385Arg variant was identified in 14 of 235 patients with Parkinson's disease (5.69%), but not in 214 unrelated healthy controls. Multivariate analysis indicated the frequency of Gly2385Arg variant in the female patients with early age at onset is higher than their male counterparts. The founder haplotype analysis showed the variant carriers shared the same founder. Clinically, the LRRK2 Gly2385Arg carriers presented with classical Parkinson's disease symptoms. Our study indicates that the LRRK2 Gly2385Arg variant is a potential ethnic-specific genetic risk factor of Parkinson's disease within Chinese Han ethnicity. 相似文献
83.
目的对比观察剖腹产选用连续硬膜外麻醉时,辅助麻醉用药镇静及对牵拉反应抑制效果。方法选择行剖腹产手术产妇60例在硬膜外麻醉效果满意后,随机分两组各30例。Ⅰ组:哌替啶50mg,异丙嗪25mg单次静注。Ⅱ组:氯胺酮28.5mg,咪达唑仑1.42mg单次静注。分组记录用药前,1min,5min,15min及术毕产妇SpO2、RR、HR、MAP值;记录手术时间、术毕苏醒例数、术中恶心躁动例数;记录术毕产妇OAA/S评分;记录新生儿娩出时Apger评分。结果两组术中镇静、抑制牵拉反应效果均满意。两组RR、BP、HR、SpO2均平稳;术毕两组OAA/S评分均为4-5分;新生儿Apger评分均正常。结论两组方法效果确切,对产妇和新生儿安全。 相似文献
84.
分析了1984~1993年期间47例经治的急性视神经炎患者。治疗应用地塞米松10mg、青霉素480万u静脉点滴7天,随后减量。结果治愈35例(74.47%),好转6例(12.76%),无效2例(4.26%),转院及自动出院各2例。47例中11例治疗效果不好者行筛窦开放术后,视力逐渐恢复正常,显示筛窦开放术有其临床实用价值。 相似文献
85.
86.
目的 利用聚合酶链反应的酶联免疫吸附试验 ,探讨检测自排尿脱落细胞端粒酶活性在膀胱癌早期诊断中的意义。方法 应用 (端粒酶重复序列扩增基础上PCR -ELISA)TRAP -PCR -ELISA方法检测 5 8例膀胱癌患者、 2 5例良性血尿患者及 30例健康体检者尿脱落细胞的端粒酶活性。结果 (1) 5 8例膀胱癌患者尿脱落细胞阳性率为 81 0 % (4 7/ 5 8) ,2 5例良性血尿患者及健康体检者均无端粒酶活性表达。 (2 )TRAP -PCR -ELISA方法检测膀胱癌患者尿脱落细胞端粒酶活性与常规尿细胞形态学镜检间的差别有非常显著性意义 (P <0 0 1)。结论 尿脱落细胞端粒酶活性的检测法对膀胱癌患者的阳性检出率较常规尿细胞形态学方法高 ,可用于膀胱癌的早期诊断。 相似文献
87.
Ivermectin for the chemotherapy of bancroftian filariasis: a meta-analysis of the effect of single treatment 总被引:4,自引:4,他引:0
Wu-chun Cao Catharina P. B. Van der Ploeg Anton P. Plaisier I. J. Sivera van der Sluijs J. Dik F. Habbema 《Tropical medicine & international health : TM & IH》1997,2(4):393-403
Summary The efficacy and safety of ivermectin in the treatment of filariasis due to Wuchereria bancrofti was assessed by a meta-analysis of the results from 15 published clinical trials. Seven hundred and forty-eight microfilaraemic patients were enrolled in 7 dose-finding and 8 comparative studies. Administered as a single dose, ivermectin induced nearly complete clearance of microfilariae from the blood from the first day to 30 days post-treatment, followed by gradual recurrence of microfilaraemia and increase in its intensity. Higher doses of ivermectin showed greater clearance effects and maintained lower microfilaraemia levels for a longer time. The adverse reactions caused by the drug were flu-like, transient, generally mild and well tolerated by patients. The frequency and intensity of adverse reactions were strongly associated with pretreatment microfilaria counts in the blood, but independent of dose. The findings of the meta-analysis suggest that ivermectin given at a single annual dose of 200 μg/kg body weight or higher, whether or not in combination with DEC, has great potential for therapeutic strategies to control bancroftian filariasis. 相似文献
88.
Anatomic and spiral computed tomographic study of the genial tubercles for genioglossus advancement.
Shan Kai Yin Hong Liang Yi Wen Ying Lu Jian Guan Hong Min Wu Zhen Yu Cao Dong Zhen Yu Yan Yan Huang Chun Gen Wu 《Otolaryngology--head and neck surgery》2007,136(4):632-637
OBJECTIVE: To measure and compare Chinese mandibular genial tubercles measured anatomically and with computed tomography (CT). STUDY DESIGN AND SETTING: Spiral CT scans were taken of 40 adult human skulls; the superior genial spines were measured using anatomic and CT methods. RESULTS: The height and width of the superior genial spines, mandible thickness, and distance from the menton to the inferior and superior margins of the superior genial spines were 5.82 +/- 0.71, 6.98 +/- 1.35, 11.95 +/- 1.59, 11.08 +/- 2.05, and 16.91 +/- 2.30 mm from anatomic measurements and 6.17 +/- 0.71, 7.01 +/- 1.13, 12.19 +/- 1.64, 10.41 +/- 1.55, and 15.73 +/- 2.12 mm using spiral CT, respectively. The anatomic and CT measurements were correlated. CONCLUSION: Spiral CT of the genial tubercles can help locate the osteotomy in genioglossus advancement. SIGNIFICANCE: This study acquired reference data on Chinese genial tubercles demonstrating that CT measurements of the genial tubercles reflect their anatomy, which should allow accurately locate the osteotomy. 相似文献
89.
We detected the presence and distribution of HBcAg in the liver by immunohistochemistry (ABC method) and the presence of HBV-DNA in serum (spot hybridization) and anti-HBe in serum (ELISA) from 59 cases of hepatitis B hospitalized in our hospital, including 47 cases of CAH, 5 cases of CPH, and 7 cases of subacute fulminant hepatitis. 1. HBcAg in the liver was detected in 25 out of 47 cases (53%) of CAH, in 2 out of 5 cases of CPH and in 4 out of 7 cases of subacute fulminant hepatitis. The total percentage was 53% (31/59). 2. There was no positive correlation between HBV replication activity and liver disease activity (P greater than 0.05). Our results did not support the hypothesis that suggests a direct cytopathic effect of HBV. Oppositely, the fact was that the presence, the amount and the patterns of HBcAg in the liver, and the presence of HBV-DNA in serum were predominant in mild CAH compared with those in severe CAH, predominant in CAH without cirrhosis compared with those in CAH with cirrhosis. There was a tendency of inverse correlation between HBV replication activity and liver disease activity. The results above were in line with the concept that HBcAg expressed on the surface of infected hepatocytes may be relevant target for T lymphocyte cytotoxicity. The results have suggested that an immune response to HBV is present, leading to the destruction of most infected cells. 3. There was a positive correlation between HBV-DNA in serum and HBcAg in the liver (P less than 0.005), indicating that HBV-DNA in serum can represent HBV replication.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
90.