全文获取类型
收费全文 | 313篇 |
免费 | 24篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 28篇 |
妇产科学 | 3篇 |
基础医学 | 34篇 |
口腔科学 | 11篇 |
临床医学 | 46篇 |
内科学 | 86篇 |
皮肤病学 | 3篇 |
神经病学 | 20篇 |
特种医学 | 41篇 |
外科学 | 22篇 |
综合类 | 8篇 |
预防医学 | 10篇 |
眼科学 | 4篇 |
药学 | 12篇 |
中国医学 | 1篇 |
肿瘤学 | 23篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 5篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 10篇 |
2014年 | 8篇 |
2013年 | 14篇 |
2012年 | 8篇 |
2011年 | 7篇 |
2010年 | 21篇 |
2009年 | 26篇 |
2008年 | 5篇 |
2007年 | 15篇 |
2006年 | 12篇 |
2005年 | 4篇 |
2004年 | 10篇 |
2003年 | 2篇 |
2002年 | 3篇 |
2001年 | 3篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 28篇 |
1997年 | 25篇 |
1996年 | 19篇 |
1995年 | 13篇 |
1994年 | 16篇 |
1993年 | 6篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1988年 | 10篇 |
1987年 | 5篇 |
1986年 | 9篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 2篇 |
1982年 | 10篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1926年 | 1篇 |
排序方式: 共有354条查询结果,搜索用时 0 毫秒
151.
ES Petherick S O'Meara K Spilsbury CP Iglesias EA Nelson DJ Torgerson 《BMC medical research methodology》2006,6(1):43-4
Background
A trial was commissioned to evaluate the effectiveness of larval therapy to debride and heal sloughy and necrotic venous leg ulcers. Larval therapy in the trial was to be delivered in either loose or bagged form. Researchers were concerned that resistance to larval therapy may threaten the feasibility of the trial. Additionally there was concern that the use of larval therapy may require a larger effect size in time to healing than originally proposed by the investigators. 相似文献152.
Talbot K; Ponting CP; Theodosiou AM; Rodrigues NR; Surtees R; Mountford R; Davies KE 《Human molecular genetics》1997,6(3):497-500
The Survival Motor Neuron (SMN) gene shows deletions in the majority of
patients with Spinal Muscular Atrophy (SMA), a disease of motor neuron
degeneration. To date only two missense mutations have been reported in SMN
in patients with SMA. The fact that no SMN-homologues have been forthcoming
from data-base searching has resulted in a lack of hypotheses concerning
the structural and functional consequences of these mutations. Recently SMN
has been shown to interact with heterogeneous nuclear ribonucleoproteins
(hnRNPs) suggesting a role in mRNA metabolism. We describe a novel missense
mutation and the subsequent identification of a triplicated
tyrosine-glycine (Y-G) peptide sequence at the C-terminal of SMN which
encompasses each of the three predicted amino acid sequence substitutions.
We have identified apparent orthologues of SMN in Caenorhabditis elegans
and Schizosaccharomyces pombe. These sequences retain the highly conserved
Y-G motif and provide additional support for a role of SMN in mRNA
metabolism.
相似文献
153.
154.
Genetic mapping of a major susceptibility locus for juvenile myoclonic epilepsy on chromosome 15q 总被引:5,自引:0,他引:5
Elmslie FV; Rees M; Williamson MP; Kerr M; Kjeldsen MJ; Pang KA; Sundqvist A; Friis ML; Chadwick D; Richens A; Covanis A; Santos M; Arzimanoglou A; Panayiotopoulos CP; Curtis D; Whitehouse WP; Gardiner RM 《Human molecular genetics》1997,6(8):1329-1334
The epilepsies are a group of disorders characterised by recurrent seizures
caused by episodes of abnormal neuronal hyperexcitability involving the
brain. Up to 60 million people are affected worldwide and genetic factors
may contribute to the aetiology in up to 40% of patients. The most common
human genetic epilepsies display a complex pattern of inheritance. These
are categorised as idiopathic in the absence of detectable structural or
metabolic abnormalities. Juvenile myoclonic epilepsy (JME) is a distinctive
and common variety of familial idiopathic generalised epilepsy (IGE) with a
prevalence of 0.5- 1.0 per 1000 and a ratio of sibling risk to population
prevalence (lambda(s)) of 42. The molecular genetic basis of these familial
idiopathic epilepsies is entirely unknown, but a mutation in the gene
CHRNA4, encoding the alpha4 subunit of the neuronal nicotinic acetylcholine
receptor (nAChR), was recently identified in a rare Mendelian variety of
idiopathic epilepsy. Chromosomal regions harbouring genes for nAChR
subunits were therefore tested for linkage to the JME trait in 34
pedigrees. Significant evidence for linkage with heterogeneity was found to
polymorphic loci encompassing the region in which the gene encoding the
alpha7 subunit of nAChR (CHRNA7) maps on chromosome 15q14 (HLOD = 4.4 at
alpha = 0.65; Z(all) = 2.94, P = 0.0005). This major locus contributes to
genetic susceptibility to JME in a majority of the families studied.
相似文献
155.
Purification and characterization of 26S proteasomes from human and mouse spermatozoa 总被引:4,自引:2,他引:4
Tipler CP; Hutchon SP; Hendil K; Tanaka K; Fishel S; Mayer RJ 《Molecular human reproduction》1997,3(12):1053-1060
We purified by fractionation on 10-40% glycerol gradients, 26S proteasomes
from normal human spermatozoa. These proteasomes, which participate in the
ATP-dependent degradation of ubiquitinated proteins, share a similar
sedimentation coefficient to those purified from other human tissues.
Fluorogenic peptide assays reveal they have chymotrypsin, trypsin and
peptidyl-glutamyl-like peptide hydrolysing activities; the chymotrypsin
activity is ablated by the specific 26S proteasome inhibitor MG132.
Confirmation that these large proteases are 26S proteasomes is provided by
detection of the 20S proteasome subunits HC2, XAPC7, RN3 and Z and
regulatory ATPases MSS1, TBP1, SUG1 and SUG2 by Western analyses with
monoclonal antisera. These antigens are found only in the gradient
fractions enriched in proteolytic activities. We have also shown that,
although mature spermatozoa from mice have considerably reduced amounts of
a ubiquitin-conjugating enzyme (E2) and ubiquitin-protein conjugates in
comparison with less mature germ cells, they retain relatively high values
of 26S proteasome activity. This suggests that proteasomes may have further
roles to play in normal sperm physiology.
相似文献
156.
V García‐García A Bascones‐Martínez AI García‐Kass CP Martinelli‐Kläy R Küffer E Álvarez‐Fernández T Lombardi 《Oral diseases》2013,19(1):65-72
Oral Diseases (2012) 19 , 65–72 Objective: Heat‐shock protein 27 (hsp27) has been implicated in several biological events. In this experimental study, we aimed at analysing, for the first time, the expression of hsp27 in the diverse stages of oral lichen planus (OLP) lesions. Materials and methods: Thirty‐six biopsy specimens of patients with OLP and 10 of healthy patients were selected. OLP specimens were divided into three groups: G1 – moderate or mildly active OLP; G2 – active or moderately active atrophic OLP; G3 – mild or inactive atrophic OLP. Hsp27 expression was analysed by immunohistochemistry (staining intensity and percentage of stained cells), and results of staining were compared between the different groups. Gender, age and anatomical location were also studied. Results: In the basal layer, an increase of hsp27 expression in both G2 and G3 was observed when compared to G1 and control group. In contrast, a decrease of hsp27 expression in the superficial layer was observed in all groups when compared to control group. Conclusion: The increased expression of Hsp27 in the basal layer observed during the OLP evolution and the less staining in the superficial layers in all cases of OLP suggest that hsp27 may have a role in the OLP pathogenesis. 相似文献
157.
Omuro A Chan TA Abrey LE Khasraw M Reiner AS Kaley TJ Deangelis LM Lassman AB Nolan CP Gavrilovic IT Hormigo A Salvant C Heguy A Kaufman A Huse JT Panageas KS Hottinger AF Mellinghoff I 《中国神经肿瘤杂志》2013,(2):131
BACKGROUND:In this phase II trial,we investigated the efficacy of a metronomic temozolomide schedule in the treatment of recurrent malignant gliomas(MGs).METHODS:Eligible patients received daily temozolomide(50 mg/m2)continuously until progression.The primary endpoint was progression-free survival rate at 6 months in the glioblastoma cohort(N=37).In an exploratory analysis,10 additional recurrent grade III MG patients were enrolled.Correlative studies included evaluation of 76 frequent mutations in glioblastoma(iPLEX assay,Sequenom)aiming at establishing the frequency of potentially"drugable"mutations in patients entering 相似文献
158.
Edílson Damke Joyce K Tsuzuki Diógenes AG Cortez Izabel CP Ferreira Thâmara A Bertoni Márcia R Batista Lucélia Donati Terezinha IE Svidzinski Márcia EL Consolaro 《BMC complementary and alternative medicine》2011,11(1):35
Background
Study of in vivo antifungal activity of the hydroalcoholic extract (HE) and n-BuOH extract (BUTE) of Sapindus saponaria against azole-susceptible and -resistant human vaginal Candida spp. 相似文献159.
160.
CP Charalambous C Mosey E Johnstone P Akimau TK Gullett I Siddique RA Wilkes 《Annals of the Royal College of Surgeons of England》2009,91(7):596-598