Proteomic studies of early-stage and advanced ovarian cancer patients

ObjectivesThe objectives of this study were to evaluate the diagnostic value for ovarian cancer using proteomic pattern established by surface-enhanced laser desorption/ionization (SELDI-TOF-MS) profiling of plasma proteins coupled with support vector machine (SVM) data analysis, and to investigate whether the proteomic pattern established by advanced ovarian cancer could be used for diagnosis of early-stage ovarian cancer patients.MethodsThe study included 44 ovarian cancer patients (11 early-stage and 33 advanced ovarian cancer patients) and 31 age-matched non-cancer controls. SELDI-TOF-MS coupled with SVM analysis was performed to establish a proteomic pattern to discriminate 33 advanced ovarian cancer patients from 31 non-cancer controls. A blind test, including 11 early-stage ovarian cancer cases, was performed to investigate whether proteomic pattern established by advanced ovarian cancer could be used for diagnosis of early-stage ovarian cancer patients.ResultsA seven-peak proteomic pattern was established which discriminated 33 advanced ovarian cancer patients from 31 non-cancer controls effectively. A sensitivity of 93.94% (31/33) and a specificity of 93.55% (29/31) were yielded from the proteomic pattern. Among the 7 protein peaks, 5 with mass charge ratio (m/z) 4099 Da, 5488 Da, 4144 Da, 4479 Da and 3940 Da were up-regulated, while 2 peaks, with m/z 13 783 Da and 8588 Da were down-regulated in the advanced ovarian cancer group compared with non-cancer control group. After blind test, 9 of 11 early-stage ovarian cancer patients were successfully diagnosed with the accuracy of 81.82% (9/11).ConclusionsThis study demonstrated that SELDI-TOF-MS coupled with SVM is effective in distinguishing protein expression between ovarian cancer and non-cancer plasma and it may be feasible to diagnose early-stage ovarian cancer using proteomic pattern established by advanced ovarian cancer. The gained and lost protein peaks in plasma may exist in both early-stage and advanced ovarian cancer plasma. Further studies should be performed using larger sample numbers.     

细胞周期调控基因p21和p27单核苷酸多态性与卵巢上皮性癌的关系

目的 探讨细胞周期调控基因p21和p27的单核苷酸多态性(SNP)与卵巢上皮性癌(卵巢癌)发病风险的关系.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测234例卵巢癌患者(卵巢癌组)和284例健康妇女(对照组)p21基因C/T和p27基因V/G SNP位点基因型和等位基因的频率分布.结果 (1)对照组妇女p21基因的C/C、C/T和T/T基因型频率分别为34.2%、49.6%和16.2%,C和T等位基因频率分别为59.0%和41.0%;卵巢癌组患者3种基因型频率分别为28.2%、53.0%和18.8%,C和T等位基因频率分别为54.7%和45.3%.两组基因型频率和等位基因频率分别比较,差异均无统计学意义(P＞0.05).3种基因型频率在4种病理类型的卵巢癌中的分布有明显差异(P=0.02),C/C基因型降低子宫内膜样癌的发病风险(OR为0.56,95%CI为0.32～0.98).(2)对照组妇女p27基因V/V、V/G和G/G基因型频率分别为88.4%、10.9%租0.7%,V和G等位基因频率分别为93.8%和6.2%;卵巢癌组患者的基因型频率分别为93.6%、5.1%和1.3%,V和G等位基因频率分别为96.2%和3.8%.两组基因型频率分布比较,差异有统计学意义(P=0.04),等位基因频率分布比较,差异则无统计学意义(P=0.09).与V/G和G/G基因型比较,V/V基因型增加卵巢癌的发病风险(OR为1.92,95%CI为1.02～3.63).结论 p21基因C/T多态性的C/C基因型可能降低子宫内膜样癌的发病风险,p27基因的V/V基因型可能是卵巢癌发病的潜在危险因素.     

泛发性无菌性脓疱病长期随访1例

报告1例随访观察26年的疱疹样脓疱病。患者女,37岁。3岁开始发病,反复发作,1980—2005年共发病15次。1992年妊娠期间皮损加重,未予特殊处理,顺产一健康男婴。1993—2005年共发病8次。发作不重时仅予对症处理,一般2～3周皮损消退;发作严重时则采用雷公藤多苷、糖皮质激素治疗,疗效显著。诊断倾向于疱疹样脓疱病。     

甲真菌病临床评分指数计算公式的推导和计算尺的研制

目的：建立甲真菌病临床评分指数（SCIO）的计算公式和计算尺。方法：根据甲真菌病的发展规律和系统抗真菌治疗甲真菌病的原理,按Sergeev评分规则,应用数学分析方法进行推导和设计计算尺。结果：SCIO计算尺由一直尺和其上的一游标组成。直尺上刻有两组带有刻度的直线,滑动直尺上的游标,即可通过游标上的垂直线读出所要求的SCIO值。结论：SCIO计算游标尺具有使用方便、便于携带的优点。     

皮肤弹性过度

报告1例皮肤弹性过度。患儿男,16岁。全身皮肤脆性增加,弹性过度,关节伸展过度14年。皮损组织病理检查示真皮组织变薄,胶原纤维疏松。弹性纤维染色示真皮上、中部弹性纤维数量增加。诊断:皮肤弹性过度。     

表皮囊肿并发毛母质瘤

报告2例表皮囊肿并发毛母质瘤。例1,男,34岁。右侧大腿上方有一灰褐色肿块,直径4cm。皮损组织病理检查示表皮囊肿的囊壁不完整,囊肿中央和周边均为成片的影细胞,囊肿中央的影细胞内可见许多小而圆的嗜伊红角化中心,提示向毛母质分化,未见嗜碱性细胞。例2,女,20岁。右上臂暗蓝色、质硬的皮下结节,直径1cm。皮损组织病理检查示表皮囊肿与毛母质瘤同时存在于真皮内,相互独立,毛母质瘤内可见嗜碱性细胞和影细胞。2例患者均未出现加德纳综合征。     

应用二维超声方法评价高血压血管内皮功能

目的:对高血压1～2级无心血管其他危险因素病人的血管内皮功能作一评估,并探讨高血压对内皮功能的影响及其可能机制。方法:研究对象30例,高血压病人和正常人各15例。应用高分辨二维超声方法检测反应性充血前后肱动脉直径,比较两组的血流介导血管舒张测定百分数(FMD)功能以及与血压之间的关系;同时比较两组血清氧化低密度脂蛋白(ox-LDL)、一氧化氮(NO)及内皮素-1(ET-1)的水平。结果:①高血压病人FMD(10.79%)明显低于对照组(19.61%),P<0.01。②相关分析显示,FMD%分别与收缩压(r=-0.446,P<0.01)、舒张压(r=-0.645,P<0.01)呈负相关。③高血压病人血清ox-LDL、ET-1显著高于对照组(P<0.01),而NO则明显低于对照组(P<0.01)。结论:高血压病早期虽无明显动脉硬化,但已存在血管内皮功能的损伤。