Intervention Effects of Jianpi Liqi Huoxue Decoction (健脾理气活血汤) on Lipid Peroxidative Liver Injury by Alcohol

Objective: To study the intervention effects of Jianpi Liqi Huoxue Decoction ( 健脾理气活血汤, JLHD) on lipid peroxidative liver injury induced by alcohol. Methods: The rat alcoholic model of liver disease (ALD) induced by Lieber-DeCarli liquid diet was established. Thirty-two male SD rats were randomly divided into 4 groups: the normal group ( n = 5), the control group ( n = 9), the model group ( n = 9) and the JLHD group (n = 9). From the 4th week after modeling, the rats were given JLHD or distilled water by gastrogavage respectively, and the samples of blood and liver tissues were taken out from the rats for determination by the end of the 8th week. The hepatic pathological changes were observed with HE staining; the liver injury related indices, including activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, γ-glutamyl transpeptidase (γ-GT) activity and triglyceride (TG) content in liver tissues, as well as the lipid peroxidation related indices, including malonaldehyde (MDA) content and nitric oxide synthase (NOS) activity in liver tissue, serum Fe2 level, and the anti-peroxidation capacity related indices, including superoxide dismutase (SOD) activity, glutathion ( GSH) content and reactive oxygen species (antiROS) activity in liver tissues were determined. Results: ( 1 ) There were obvious figures of fatty degeneration and inflammatory infiltration in liver tissues of the model group. As compared with the control group, in the model group, the activity of ALT and AST, and Fe2 content in serum, γ-GT and NOS activity, TG and MDA content in liver tissues were significantly higher ( P＜0.01 ), while the activity of SOD, GSH and anti-ROS in liver tissues were significantly lower ( P＜0.01 ). (2) The fatty degeneration and inflammatory infiltration of liver tissues in the JLHD group were significantly lessen as compared with those in the model group; and the abnormalities of all the indexes revealed in the model rats were restored to certain extent in the JLHD group,and especially significant were the levels of ALT activity, MDA content and Fe2 , which were nearly normal.Conclusion: JLHD has significant effects against alcoholic liver injury, the acting mechanism of which is likely to be related with its anti-lipid peroxidative effect.     

校企联合开展影像学实验室建设的探讨与实践

本文提出了校企共建、实现双赢的高校专业实验室发展的新模式,并成功地应用于我院影像技术实验室的建设与改革的实践中.通过校企共建实验室,使实验室建设有了超常规的发展,产生了较大的社会效益和经济效益,实现了校企双赢的目的.     

归苓片改善动物学习记忆功能的研究

目的研究归苓片(FBD)促进动物学习记忆功能的作用。方法小鼠ig给予35、70、140mg/kg FBD,连续1周,观察FBD对东莨菪碱所致小鼠记忆获得障碍、亚硝酸钠所致小鼠记忆巩固不良和45%乙醇致小鼠记忆再现障碍的影响,并观察FBD对大鼠双侧颈总动脉结扎所致的慢性脑部供血不足而产生的记忆障碍的改善作用。结果FBD对由东莨菪碱所致的小鼠记忆获得障碍、亚硝酸钠所致的小鼠记忆巩固不良和45%乙醇所致的小鼠记忆再现障碍均有明显的改善作用;并可改善大鼠双侧颈总动脉结扎所致的慢性脑部供血不足而产生的记忆障碍。结论FBD可促进动物的学习记忆功能。     

山东某市二次供水水质卫生状况分析

目的：探讨二次供水系统中蓄水池（箱）等蓄水设施对水质的影响状况。方法：对2001～2004年滨州市区直接供水与二次供水23项卫生监测指标进行分析。检测方法按GB5750—85《生活饮用水标准检验方法》，评价依据按GB5749—85《生活饮用水卫生标准》。监测指标包括浑浊度、总铁、细菌总数、大肠菌群、氨氮、亚硝酸盐氮、硝酸盐氮。结果：二次供水合格率仅为12．2％，与城市供水管网末梢水水质相比较差异有统计学意义。结论：二次供水污染严重，应引起高度重视。     

大肠杆菌气管注入致急性肺损伤的实验研究

目的：建立气管注入细菌致兔急性肺损伤（ALI）模型,为研究细菌致ALI的致病机制及其治疗提供理论依据.方法：兔分为：细菌注入组（Ⅰ组）,生理盐水注入组（Ⅱ组）,正常对照组（Ⅲ组）.对比观察3组以下指标：血流动力学、呼吸力学、血气分析、支气管灌洗液中的细胞数、蛋白含量、肺组织湿重/干重比及病理检查.结果：Ⅰ组与Ⅱ组、Ⅲ组比较,上述指标,均有明显差异（P均＜0.05）.结论：通过直接气管注入细菌可建立ALI模型.     

工作场所空气中2-二乙氨基乙醇气相色谱测定方法的研究

[目的]建立工作场所空气中2-二乙氨基乙醇气相色谱测定方法。[方法]采用活性炭管吸附空气中2-二乙氨基乙醇,经二硫化碳解吸后气相色谱仪检测。[结果]本研究方法测定空气中2-二乙氨基乙醇线性范围5.0~200μg/ml,相对标准偏差(RSD)2.6%~6.4%,回归方程Y=5.34X-7.32,r=0.9998;方法最低检出浓度0.23mg/m(3采样体积3L计),解吸效率86.1%~92.8%,相对标准偏差1.9%~5.8%;穿透容量(BTV)6.57mg;2-二乙氨基乙醇室温放置5d,下降率9.5%;采样效率>90%。[结论]建立了工作场所空气中2-二乙氨基乙醇气相色谱测定方法,具有灵敏度高、操作简便、重现性好、准确可靠,适合工作场所空气中2-二乙氨基乙醇的测定。     

耐多药结核分枝杆菌三种基因的快速检测

目的 :探讨耐多药结核分枝杆菌耐药基因突变与耐药性的关系。方法 :采用 PCR和 PCR- DS技术对 5 7例耐多药结核临床分离株进行 kat G、rpo B和 emb B基因检测和序列分析。结果 :耐 INH(kat G)、RFP(rpo B)、EMB(emb B)基因突变率分别为 6 3.8%、90 .7%、37.1% ,其中同时耐 INH(kat G)和 RFP(rpo B)基因突变率为 5 4 .1% ,同时耐三种药的基因突变率为 6 5 .6 %。结论 :PCR- DS法对耐两种或两种以上药物的结核检出率较高 ,与传统药敏试验互相弥补 ,对临床用药有指导意义。     

HPLC-MS同时测定人血浆中吡格列酮及其活性代谢产物的浓度

 目的建立高效液相色谱质谱联用测定人血浆中吡格列酮及其活性代谢产物M-Ⅲ(酮类衍生物)和M-Ⅳ(羟化衍生物)的方法。方法采用WatersXTerraTN C₁₈色谱柱(2.1mm×150mm,3.5μm),Phenomenex C₁₈保护柱,柱温50℃,流动相:乙腈-30mmol·L^-1醋酸铵溶液(含0.1%的甲酸和0.05%的三氟醋酸)(35:65),流速0.22mL·min^-1。质谱采用电喷雾电离源正离子模式(ESI⁺),选择性监测质荷比(m/z)357.4(吡格列酮),358.2(内标),371.5(M-Ⅲ),373.2(M-Ⅳ)的准分子分子离子峰;血浆样品经盐酸酸化后,采用叔丁基甲醚-氯仿提取,内标法定量。结果吡格列酮,M-Ⅲ和M-Ⅳ分别在11.16～1748.60,3.33～520.50,5.00～687.50ng·mL^-1内线性良好(r≥0.9997),最低检测浓度分别为2.90,1.10,1.20ng·mL^-1,3个化合物的方法回收率均在90%-110%范围内,日间和日内RSD皆小于15%。结论本方法操作简单,灵敏准确,稳定性好,适用于临床吡格列酮治疗药物监测,药动学及其药物相互作用的研究。     

明黄膏质量标准的研究

目的:研究明黄膏质量标准.方法:采用薄层色谱(TLC)法对明黄膏中的主要成分大黄、黄连、苦参进行定性鉴别;高效液相色谱(HPLC)法测定明黄膏中大黄酚的含量.结果:TLC色谱中均能明显地检出大黄、黄连、苦参;HPLC法测得本品中大黄酚的含量为0.267～0.308 mg·g-1;在10.02～100.16 mg·L-1的范围内,溶液的浓度与峰面积呈良好线性关系,r=0.999 9;加样平均回收率为98.48%(n=6),RSD为1.20%.结论:本品定性、定量方法简便、准确,专属性强,质量标准能够控制该制荆的内在质量.     

南京地区22家医院2001年～2003年降糖药应用分析

目的:分析南京地区22家医院2001年～2003年降糖药的应用情况及用药趋势.方法:用金额排序法统计3年来降糖药的主要品种的应用情况,分析临床用药趋势.结果:降糖药的用药金额逐年上升;胰岛素制剂中,合资品种占明显优势的市场分额;口服降糖药中,阿卡波糖用药金额占据第1位;格列美脲、瑞格列奈、罗格列酮的构成比逐年跳跃增长;二甲双胍的用药频度最大,日均费用最低.结论:降糖药的需求量逐年递增;市场需求潜力巨大,合资品种、进口品种受到医生和患者的青睐.开发不同作用机制的质优、方便、价廉的降糖药物,将会有良好的市场前景.