全文获取类型
收费全文 | 443篇 |
免费 | 31篇 |
国内免费 | 13篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 16篇 |
妇产科学 | 8篇 |
基础医学 | 35篇 |
口腔科学 | 17篇 |
临床医学 | 54篇 |
内科学 | 101篇 |
皮肤病学 | 17篇 |
神经病学 | 2篇 |
特种医学 | 127篇 |
外科学 | 23篇 |
综合类 | 11篇 |
预防医学 | 18篇 |
眼科学 | 3篇 |
药学 | 14篇 |
肿瘤学 | 40篇 |
出版年
2023年 | 1篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 9篇 |
2017年 | 4篇 |
2016年 | 5篇 |
2015年 | 12篇 |
2014年 | 5篇 |
2013年 | 21篇 |
2012年 | 9篇 |
2011年 | 7篇 |
2010年 | 15篇 |
2009年 | 25篇 |
2008年 | 6篇 |
2007年 | 24篇 |
2006年 | 12篇 |
2005年 | 8篇 |
2004年 | 2篇 |
2003年 | 3篇 |
2002年 | 6篇 |
2001年 | 7篇 |
2000年 | 2篇 |
1999年 | 7篇 |
1998年 | 47篇 |
1997年 | 45篇 |
1996年 | 22篇 |
1995年 | 23篇 |
1994年 | 12篇 |
1993年 | 12篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 10篇 |
1988年 | 13篇 |
1987年 | 13篇 |
1986年 | 11篇 |
1985年 | 10篇 |
1984年 | 10篇 |
1983年 | 8篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 9篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1975年 | 6篇 |
排序方式: 共有487条查询结果,搜索用时 17 毫秒
131.
1临床资料1990-01/2003-12采用新型植骨方法结合天鹅型记忆接骨器[1]治疗上肢骨干骨不连93(男60,女33)例,107(肱骨50,尺桡骨28,尺骨14,桡骨11,锁骨4)根,年龄10~80(平均37.6)岁.所有病例入我院前已行1~5(平均1.7)次手术.植骨以自体髂骨为主,必要时辅以同种异体骨植骨.内固定采用我院张春才发明,以镍钛记忆合金制成,由鹅体、鹅颈、鹅翼组成的天鹅型记忆接骨器[1](Swan-like Memorablycompressive Connector , SMC). 相似文献
132.
目的 报告腹膜外途径腹腔镜前列腺癌根治术的初步体会。方法 分别于2005年4月、12月,经腹膜外途径进行腹腔镜前列腺癌根治术2例。结果 2例均获成功,首例手术时间为7小时20分钟,第2例为3小时20分钟。术中分别输红细胞悬液3单位、2单位。首例术后出现尿漏12d,术后23d带导尿管出院,半月后膀胱造影提示无尿漏后拔导尿管,站立后有尿失禁,术后4个月后尿失禁完全消失;第2例患者术后未出现尿漏,于术后10d拔除导尿管,拔管后即无尿失禁,术后15d出院。结论 腹膜外途径腹腔镜前列腺癌根治术是安全、可行的,较传统的经耻骨后径路的开放性手术及经腹腔途径的腹腔镜前列腺癌根治术均有一定的优势。 相似文献
133.
Tani M; Takenoshita S; Kohno T; Hagiwara K; Nagamachi Y; Harris CC; Yokota J 《Carcinogenesis》1997,18(5):1119-1121
Mutations in the transforming growth factor beta-type II receptor (TGFbeta
RII) gene have been detected in several types of human cancers that
represent the phenotype of genomic instability. The TGFbeta RII gene has
been mapped to chromosome 3p, on which loss of heterozygosity (LOH) was
frequently detected in both small cell lung carcinoma (SCLC) and non-small
cell lung carcinoma (NSCLC). To investigate whether the TGFbeta RII gene on
3p22 is inactivated in lung cancers, we examined 35 sporadic lung cancers
(15 SCLC and 20 NSCLC) with LOH on 3p for mutations of the TGFbeta RII
gene. We previously produced eight intron based primer pairs for mutational
analysis of the entire coding region of the TGFbeta RII gene. Using these
primers, we screened for mutations of the TGFbeta RII gene by polymerase
chain reaction-single strand conformational polymorphism (PCR-SSCP)
analysis. A mutation was detected in a case of SCLC: one base insertion in
the polyadenine tract of exon 3. This tumor showed the replication error
(RER) phenotype. There were no mutations in exons 1, 2, 4, 5, 6 and 7.
These results indicate that the polyadenine tract is a mutational hot spot
in the TGFbeta RII gene in RER positive tumors, and that TGFbeta RII
mutations occur rarely in lung cancers with LOH on chromosome 3p.
相似文献
134.
Serum, plasma and paraffin-embedded tissues as sources of DNA for studying cancer susceptibility genes 总被引:1,自引:1,他引:1
The ability to isolate DNA from archived human serum, plasma and
paraffin-embedded human tissues enhances opportunities to study breast,
lung and other cancer risk factors. We report herein a simple and fast
protocol for the extraction of genomic DNA from these sources. Using a
phenol-based extraction method, the recovery for DNA is quantitative and
reproducible. DNA yields in serum (250 microl) were between 162 and 1060 ng
(n = 18 subjects), in plasma (250 microl) were between 165 and 375 ng (n =
5 subjects) and in embedded tissues (5-microm thick sections for ethanol
fixed, and between 5- and 20-microm sections for formaldehyde fixation)
were between 1 microg and 11.7 microg (n = 32 subjects). The extraction
method was combined with newly designed PCR- based assays for cancer
susceptibility marker genes such as CYP1A1 (exon 7), CYP2E1 (Dra1, Rsa1),
GSTM1 and NAT2 [NAT2*5A (C481T), NAT2*6A (G590A), NAT2*7A (G857A)].
Genotyping results from the serum and paraffin-embedded tissues compared
favorably to results from archived freshly frozen tissues, where
concordance was 98% for serum, 100% for ethanol-fixed embedded tissues, and
97% for formaldehyde-fixed and paraffin-embedded tissues. This facile
method will allow for the use of archived tissue samples of prospective
cohort and other studies where intact DNA was not previously available.
相似文献
135.
TYW Hon CC Chan T Loke CS Chan 《Journal of Medical Imaging and Radiation Oncology》1998,42(3):258-261
A case of torsion of the wandering spleen with splenic infarction is reported. The medical imaging studies performed with typical findings are described. Case reports have been reviewed. Although rare, torsion of the spleen remains an important differential diagnosis in patients presenting with acute abdominal pain. Early intervention is necessary to reduce the risk of splenic infarction and other complications, and an increased awareness of the condition together with the use of appropriate medical imaging can lead to the correct diagnosis more readily. 相似文献
136.
137.
Mutation analysis of the Smad2 gene in human colon cancers using genomic DNA and intron primers 总被引:2,自引:0,他引:2
Takenoshita S; Tani M; Mogi A; Nagashima M; Nagamachi Y; Bennett WP; Hagiwara K; Harris CC; Yokota J 《Carcinogenesis》1998,19(5):803-807
In mammals, one of the Mad homologues, Smad2, was reported to be a mediator
of TGF-beta signaling, and was found mutated in some cases of colon and
lung cancers. To extend the analysis of this gene, we previously
investigated the genomic organization of the human Smad2 gene and defined
the structure of 12 exons and flanking introns. In this study, we designed
11 sets of intron-based primers to examine the entire coding region of the
Smad2 gene. By the PCR-SSCP method using these primers, we screened genomic
DNA sequences of colorectal cancers for mutations of the Smad2 gene. Though
there was no mutation within all exons of the Smad2 gene, two of 60
sporadic colorectal cancers displayed deletions in the polypyrimidine tract
preceding exon 4. Deletions of this region were also detected in colon
cancer cell lines, and were clustered within cells exhibiting
microsatellite instability. Deletions in the polypyrimidine tract had
various effects on pre-mRNA splicing, but had no effect on the splicing of
the Smad2 gene in these cases. However, our data support the idea that the
polypyrimidine tract in the splicing acceptor site is a target of mutations
in mismatch repair-deficient tumors.
相似文献
138.
Yen CC; Hsieh RK; Chiou TJ; Liu JH; Fang FS; Wang WS; Tung SL; Tzeng CH; Chen PM 《Japanese journal of clinical oncology》1998,28(2):129-133
The anti-emetic efficacy of a combination of tropisetron and dexamethasone
was studied in 33 patients who underwent bone marrow transplantation.
Another 50 patients receiving conventional anti-emetic therapies in bone
marrow transplantation served as control. On the first and second days of
preconditioning chemotherapy, 51% and 36% respectively of patients in the
tropisetron and dexamethasone group did not experience vomiting, compared
with only 12% and 10% of control group patients (P < 0.001). The mean
number of episodes of vomiting in the tropisetron and dexamethasone group
was also significantly lower than in the control group (0.97+/-1.65 vs
3.50+/-2.45 and 1.30+/-1.40 vs 4.44+/-2.91 respectively, both P <
0.001). Control of vomiting in the two groups was not significantly
different during days 3-6. Analysis of patients receiving busulfan and
cyclophosphamide as the preconditioning regimen still showed better
anti-emetic control in the tropisetron and dexamethasone group than in the
control group on the first two days of treatment (total control rate 33.3%
vs 6.5% and 44.4% vs 12.9% respectively, P < 0.001). Patients given
tropisetron and dexamethasone combination more frequently suffered from
dizziness and burning sensation of the chest. However, diarrhea and
extrapyramidal symptoms were the most frequent adverse effects seen after
using conventional anti-emetic combination. The combination of tropisetron
and dexamethasone was thus superior to conventional anti-emetic
combinations in preventing vomiting during preconditioning period of bone
marrow transplantation. The adverse effects of this combination were
minimal and well tolerated by patients.
相似文献
139.
Lee CC; Masuda C; Yamamoto S; Wanibuchi H; Ikemoto S; Sugimura K; Nakatani T; Wada S; Kishimoto T; Fukushima S 《Japanese journal of clinical oncology》1998,28(3):227-232
A case featuring a well differentiated adenocarcinoma mixed with a
transitional cell carcinoma (TCC) arising in the renal pelvis of a
63-year-old woman is presented. Daughter tumors, located in the ureter and
the uretero-vesical junction, were entirely TCC in character.
Immunohistochemical assessment of cell cycle-related proteins revealed
overexpression of cyclin D1 but reduced p21WAF1/Cip1 or PCNA expression in
the adenocarcinomatous regions. Conversely, expression of p21WAF1/Cip1 and
PCNA was high in the TCC components. Immunohistochemical staining for p53
was negative and PCR-SSCP analyses confirmed the absence of any mutation.
Therefore, assessments on the altered expression of cell cycle-related
elements may contribute to our understanding of tumor biology in
adenocarcinomas and TCCs of the renal pelvis and to identifying the
similarities and differences between the two different cell types.
相似文献
140.
Chiou TJ; Tung SL; Hsieh RK; Wang WS; Yen CC; Fan FS; Liu JH; Chen PM 《Japanese journal of clinical oncology》1998,28(5):318-322
BACKGROUND: The efficacy of the treatment of advanced gastric cancer is not
very good. The response rate to the original
etoposide--leucovorin--5-fluorouracil (ELF) treatment is 53% with tolerable
side effects. Whether increasing the dose intensity by prolonging the
duration of infusion with 5-fluorouracil (5-FU) and leucovorin (LV) from 3
to 5 days for advanced or metastatic gastric cancer patients would enhance
the efficacy but not increase side effects is still unknown. METHODS:
Thirty-six advanced or metastatic gastric cancer and chemotherapy-naive
patients with measurable or evaluable diseases were scheduled to receive
intravenous etoposide 100 mg/m2/day on days 2-4, LV 300 mg/m2/day
intravenously and 5-FU 500 mg/m2/day intravenously on days 1-5, every 4
weeks. All patients who received at least two courses of chemotherapy were
evaluated for tumor response, survival and response duration and toxicity
according to the WHO criteria. RESULTS: Thirteen patients showed a
response, including five with complete response (CR). The overall response
rate was 36% (95% confidence interval, CI, 20-52%) in the whole group and
46% (95% CI 28-66%) in the 28 patients with measurable disease. The median
progression-free interval and overall median survival time were 5 and 7
months, respectively. The most frequent toxicity was alopecia (grade I/II
56.3%). The incidence of grade III or greater myelosuppression was 5.9%. No
treatment-related death occurred. CONCLUSIONS: The efficacy of the modified
ELF by increasing the dosages of 5-FU and LV is not superior to the results
of the original regimen, yet it is a relatively safe and tolerable
combination regimen for advanced gastric cancer.
相似文献