全文获取类型
收费全文 | 1332篇 |
免费 | 112篇 |
国内免费 | 63篇 |
专业分类
耳鼻咽喉 | 7篇 |
儿科学 | 57篇 |
妇产科学 | 4篇 |
基础医学 | 147篇 |
口腔科学 | 31篇 |
临床医学 | 106篇 |
内科学 | 266篇 |
皮肤病学 | 60篇 |
神经病学 | 92篇 |
特种医学 | 259篇 |
外科学 | 61篇 |
综合类 | 65篇 |
预防医学 | 88篇 |
眼科学 | 121篇 |
药学 | 107篇 |
肿瘤学 | 36篇 |
出版年
2022年 | 8篇 |
2021年 | 20篇 |
2019年 | 8篇 |
2018年 | 16篇 |
2017年 | 10篇 |
2016年 | 13篇 |
2015年 | 23篇 |
2014年 | 25篇 |
2013年 | 34篇 |
2012年 | 17篇 |
2011年 | 22篇 |
2010年 | 26篇 |
2009年 | 32篇 |
2008年 | 36篇 |
2007年 | 55篇 |
2006年 | 37篇 |
2005年 | 38篇 |
2004年 | 23篇 |
2003年 | 26篇 |
2002年 | 22篇 |
2001年 | 34篇 |
2000年 | 24篇 |
1999年 | 28篇 |
1998年 | 57篇 |
1997年 | 66篇 |
1996年 | 57篇 |
1995年 | 62篇 |
1994年 | 36篇 |
1993年 | 44篇 |
1992年 | 26篇 |
1991年 | 26篇 |
1990年 | 28篇 |
1989年 | 54篇 |
1988年 | 50篇 |
1987年 | 49篇 |
1986年 | 29篇 |
1985年 | 34篇 |
1984年 | 28篇 |
1983年 | 25篇 |
1982年 | 20篇 |
1981年 | 31篇 |
1980年 | 33篇 |
1979年 | 31篇 |
1978年 | 10篇 |
1977年 | 24篇 |
1976年 | 22篇 |
1975年 | 15篇 |
1974年 | 16篇 |
1973年 | 14篇 |
1972年 | 6篇 |
排序方式: 共有1507条查询结果,搜索用时 187 毫秒
31.
32.
0 引言 人类免疫缺陷病毒 (human immunodeficiencyvirus,HIV) - 1编码的反式激活蛋白 TAT具有独特的跨膜运转方式 ,而且有转导速度快 ,效率高的特点 ,被称为蛋白转导结构域 (protein transduction domain,PTD) [1 ,2 ] .本研究用PCR扩增了慢性粒细胞白血病慢粒 bcr/ abl融合蛋白的基因片段 ,在其 5′端融合 PTD结构域的编码区后在大肠杆菌中进行了表达 .表达产物经纯化后 ,加入培养的 HL 6 0细胞 ,表达的蛋白可直接进入细胞内 .这一结果为用外源蛋白负载(L oading)免疫细胞提供了新的途径 .1 材料和方法1.1 DNA重组 人工合… 相似文献
33.
34.
The prevalence of microalbuminuria was assessed in 50 patients of non-insulin dependent diabetes mellitus. The mean age of patients was 52.1 ± 11.6 years and the duration of diabetes was 8.3 ± 6.8 years. Twenty (40%) patients had microalbuminuria. Microalbuminuria was more common in patients with a longer duration of diabetes (more than 5 years), a poor glycaemic control, and higher systolic blood pressure.KEY WORDS: Microalbuminuria, Diabetes mellitus, Diabetic nephropathy, Chronic renal failure 相似文献
35.
36.
A nationwide study of intracellular drug metabolite concentrations in children prescribed 6-mercaptopurine for the treatment of lymphoblastic leukaemia was carried out to assess interpatient variability at a standardised dose. Nine children (2% of the total) had completely undetectable metabolites, indicative of non-compliance. Five were adolescents, but otherwise they had no obvious distinguishing characteristics. Not taking any 6-mercaptopurine at all is uncommon, but the problem cannot be predicted. The total number of children who do not comply cannot be determined from this study, but the nine children described represent only a fraction of these. 相似文献
37.
Zhao P Cao J Zhao LJ Qin ZL Ke JS Pan W Ren H Yu JG Qi ZT 《第二军医大学学报》2006,27(5):506-506
The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DHSalpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobutins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity (DTH) and CD^8+ CTL responses to N protein. 相似文献
38.
Catherine A. Brownstein Paul Wicks 《Clinical orthopaedics and related research》2010,468(10):2581-2585
Background
Osteogenesis imperfecta (OI) is an inherited connective tissue disorder with many phenotypic presentations ranging from mild to severe. It is often called “brittle bone disease.” Treatment consists of physical therapy, surgical interventions, medications and, in some cases, experimental therapies. Because treatment is not standardized and is often experimental, information on the success of different methods is usually not available or well documented. 相似文献39.
The epidemiology of hyperuricaemia and gout in Taiwan aborigines 总被引:4,自引:1,他引:4
To determine the prevalence of hyperuricaemia, gout and gout-related
factors in Central Taiwan Atayal aborigines, 342 subjects over 18 yr old
were interviewed and examined. A questionnaire was designed to screen for
signs and symptoms of gout and gout-related risk factors. Serum uric acid,
triglyceride and creatinine were measured in all subjects. The prevalence
of hyperuricaemia was 41.4% and that of gout 11.7% in aborigines. The uric
acid level was 7.9+/-1.7 mg/dl in males and 5.7+/-1.5 in females, and
differed significantly under age 70 yr (P < 0.001). Significantly
increased triglyceride, creatinine and alcoholism was found in gouty
patients compared with non-gouty patients. In 40 cases with gout, 54% had
tophi and 35% of their first- degree relatives had gout. The high
prevalence of hyperuricaemia and gout in Taiwan Atayal aborigines, a
significant family predisposition, increased creatinine level and
alcoholism suggest multiple factors affecting the hyperuricaemia.
相似文献
40.
Ortiz-Alvarez O; Cabral D; Prendiville JS; Stringer D; Petty RE; Malleson PN 《Rheumatology (Oxford, England)》1997,36(2):280-284
Two children are reported in whom intestinal pseudo-obstruction was the
initial manifestation of systemic sclerosis. Gastrointestinal symptoms and
skin changes resolved or improved in both children following treatment with
prednisone and penicillamine (case 1) or methotrexate (case 2), although
radiological changes of the gastrointestinal tract persisted at 3 and 2 yr
of follow-up, respectively.
相似文献