高效液相色谱法测定多维元素片中泛酸的含量

目的建立复合多维元素片中泛酸的含量测定方法。方法高效液相色谱法,C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-1mL·L-1磷酸水溶液(5∶95);流速:1.0mL·min-1;柱温:25℃;检测波长:200nm。结果泛酸质量浓度在1.32¹32.32μg·mL-1范围内具有良好的线性关系,相关系数r=0.999 9,平均回收率为99.3%,RSD为1.4%(n=12)。结论该方法快速简便,结果准确,适用于多维元素片中泛酸含量测定。     

提高医学院校文科生生物化学教学质量策略探讨

生物化学是医学院校学生一门重要的公共基础课。大多数学生认为生物化学这门课程内容庞杂、名词术语繁多、抽象难懂、学习难度大,尤其对于生物学科与化学学科基础薄弱的文科生来讲,学习过程吃力,学习效果不理想,期末挂科率较高。本研究从文科生学习生物化学遇到的困难及教师面临的教学现状出发,找出问题,探讨具体应对策略,旨在提高学生学习生物化学课的兴趣和效果。     

Regulation of neutrophil migration and superoxide production by recombinant tumor necrosis factors-alpha and -beta: comparison to recombinant interferon-gamma and interleukin-1 alpha

We compared the ability of recombinant human tumor necrosis factor- alpha (rHuTNF-alpha) and tumor necrosis factor-beta (rHuTNF-beta) to stimulate polymorphonuclear neutrophil (PMN) migration and superoxide production. Significant PMN migration occurred across polycarbonate filters after stimulation with rHuTNF-alpha at concentrations ranging from 10(-7) to 10(-10) mol/L and at 10(-7) to 10(-8) mol/L for rHuTNF- beta and N-formylmethionyl-leucyl phenylalanine (FMLP), whereas recombinant human interferon-gamma was only minimally active at 10(-7) mol/L and recombinant human interleukin-1 alpha was inactive at the doses tested. In addition, antibodies to rHuTNF-alpha completely inhibited rHuTNF-alpha but not rHuTNF-beta or FMLP-induced PMN migration. Combinations of rHuTNF-alpha and rHuTNF-beta (at similar molar concentrations) stimulated PMN migration levels comparable to that obtained with rHuTNF-alpha alone. Checkerboard analyses performed by placing different concentrations of rHuTNF-alpha and rHuTNF-beta above and below polycarbonate filters of microchemotaxis chambers demonstrated that rHuTNF-alpha and rHuTNF-beta stimulated both chemotactic and chemokinetic responses by PMN. Additional studies demonstrated that 1 X 10(-8) mol/L rHuTNF-alpha and 3 X 10(-9) mol/L rHuTNF-beta (which represents 10(4) U/mL of each cytokine) were similar in their ability to induce superoxide production by PMNs; however, at ten- to 100-fold lower molar concentrations (10(3) and 10(2) units), rHuTNF-alpha was significantly more active than rHuTNF-beta. At the doses tested, both cytokines were less active than phorbol myristate acetate at stimulating O2- release. The results demonstrate that rHuTNF- alpha and rHuTNF-beta differ quantitatively but not qualitatively in their effects on PMN functions in vitro and suggest that rHuTNF-beta may be less toxic than rHuTNF-alpha in vivo.     

美托洛尔缓释片治疗原发性高血压并舒张压性心力衰竭的疗效观察

目的：探讨美托洛尔缓释片治疗原发性高血压（EH）并舒张压性心力衰竭（DHF）患者的临床疗效。方法：选取2010年7月-2013年4月本院就诊的126例EH合并DHF患者作为研究对象,所有患者给予美托洛尔缓释片治疗12个月。分别观察治疗前后患者的血压、心率、VEGF和hs-CRP水平的变化,同时观察心脏结构、左室功能参数变化及心功能分级的变化情况。结果：治疗后,左室舒张末径（LVEDD）和左室收缩末径（LVESD）显著减小,二尖瓣返流现象显著减轻,左室射血分数（LVEF）、左室高峰充盈数（LVPFR）显著增加,收缩压、舒张压、脉压和心率以及VEGF和hs-CRP、N末端钠肽原（NT-proBNP）均显著降低,上述指标治疗前后比较差异均具有统计学意义（P＆lt;0.05）。结论：美托洛尔缓释片治疗EH并DHF患者,能显著改善患者的心脏功能,治疗效果显著,值得在临床上进一步推广。     

成人阻塞性睡眠呼吸暂停低通气综合征患者的人格特征分析

目的　调查成人阻塞性睡眠呼吸暂停低通气综合征（OSAHS）患者的人格特征及生活质量,了解该患病群体的心理健康情况。方法　采用明尼苏达多项人格测量表（Minnesota multiphasic personality inventory,MMPI）和简明健康状况调查表（the MOS item short from health survey,SF-36）分别对98例成人OSAHS患者及100例单纯鼾症志愿者进行问卷调查,评价OSAHS患者的人格特征及生活质量情况并进行对照性研究。结果　在MMPI调查中,OSAHS患者在抑郁、疑病、精神衰弱、情绪不稳定、精神分裂临床量表T分高于单纯鼾症组,差异具有统计学意义（P＜0.05）,其中OSAHS组中女性患者的抑郁、疑病、精神衰弱、情绪不稳定、精神分裂、社会内向临床量表T分高于男性患者,差异具有统计学意义（P <0.05）。在SF-36调查中,OSAHS患者在生理职能、一般健康状况、躯体疼痛、精力、社会功能、情感功能、精神健康7个维度的平均评分低于单纯鼾症组,差异具有统计学意义（P＜0.05）。结论  OSAHS患者有较多的心理健康问题,表现更多的易疲劳、注意缺陷,生活质量更低,OSAHS病情越重,心理健康问题越大,女性患者尤重。     

Temas de actualidad en cardiología: riesgo vascular y rehabilitación cardiaca

Cardiovascular disease develops in a slow and subclinical manner over decades, only to manifest suddenly and unexpectedly. The role of prevention is crucial, both before and after clinical appearance, and there is ample evidence of the effectiveness and usefulness of the early detection of at-risk individuals and lifestyle modifications or pharmacological approaches. However, these approaches require time, perseverance, and continuous development. The present article reviews the developments in 2013 in epidemiological aspects related to prevention, includes relevant contributions in areas such as diet, weight control methods (obesity is now considered a disease), and physical activity recommendations (with warnings about the risk of strenuous exercise), deals with habit-related psychosocial factors such as smoking, provides an update on emerging issues such as genetics, addresses the links between cardiovascular disease and other pathologies such as kidney disease, summarizes the contributions of new, updated guidelines (3 of which have recently been released on topics of considerable clinical importance: hypertension, diabetes mellitus, and chronic kidney disease), analyzes the pharmacological advances (largely mediocre except for promising lipid-related results), and finishes by outlining developments in the oft-neglected field of cardiac rehabilitation. This article provides a briefing on controversial issues, presents interesting and somewhat surprising developments, updates established knowledge with undoubted application in clinical practice, and sheds light on potential future contributions.     

脊柱化脓性感染动物模型的建立及应用进展

手术部位感染是脊柱手术后常见且非常严重的并发症,严重影响患者的身体健康。尽管手术操作无菌细致,及时给予适当的全身抗生素,但手术部位感染率仍然很高[1-2]。据报道,我国脊柱手术感染的风险从0.5%~7.8%不等[3-4]。糖尿病、肥胖、高血压等疾病显著增加脊柱术后感染,感染后治疗的费用可达10多万美元[5],大大增加了患者的经济负担。     

4种便携式血糖仪与生化分析仪的一致性和抗干扰能力的研究

目的 通过对不同便携式血糖仪与贝克曼库尔特A U 5800全自动生化分析仪(A U 5800)检测血糖结果的一致性比对和抗干扰能力的研究,为临床选择适合的血糖仪提供参考.方法 共选取4种不同检测原理的2台进口(A和B)和2台国产(C和D)血糖仪,与A U 5800同时检测不同浓度的血糖并进行比较,分析其一致性.分别检测...     

三维有限元分析在牙周夹板研究中的应用

牙周炎是口腔常见病、多发病,是牙齿松动的主要原因之一.牙周夹板一直以来是松牙固定的有效手段,它可以将多个牙连成一个整体,形成一个“多根巨牙”,共同承担咬合力,有效地分散牙周膜及牙槽骨的应力,为牙周组织的愈合创造有利条件.三维有限元分析法是研究复杂模型应力分布的生物力学方法,近年来广泛应用于牙周夹板的研究中,该方法可以逼...