芦荟凝胶多糖对急性放射性皮肤损伤模型的修复作用及机制研究

目的研究芦荟凝胶多糖基于磷脂酰肌醇3-激酶(PI3K)-蛋白激酶B(Akt)-雷帕霉素靶蛋白(mTOR)信号通路对急性放射性皮肤损伤模型的修复作用。方法选取40只雄性Wistar大鼠,10只为正常组(等体积生理盐水),其余30只建立急性放射性皮肤损伤模型,随机分为模型组、低剂量组、高剂量组,每组10只。模型组和正常组均给予等体积生理盐水干预,低剂量组、高剂量组分别以单层纱布浸润0.4 mg/mL、0.8 mg/mL的芦荟凝胶多糖外敷干预。记录创面愈合时间、放射性皮肤损伤评级,检测创面微血管密度、血管内皮生长因子(VEGF)含量,病理学观察创面组织细胞形态,Western blot检测PI3K-Akt-mTOR信号通路相关蛋白表达。结果低剂量组和高剂量组创面愈合时间均短于模型组,且高剂量组创面愈合时间显著短于低剂量组(P<0.05);与模型组相比,正常组大鼠0级较多,Ⅳ级较少;低剂量组和高剂量组大鼠Ⅳ级较少(P<0.05)。模型组、低剂量组、高剂量组创面微血管密度、VEGF、PI3K、Akt蛋白表达量低于正常组,mTOR蛋白表达量高于正常组;低剂量组、高剂量组创面微血管密度、VEGF、PI3K、Akt蛋白表达量高于模型组,mTOR蛋白表达量低于模型组;高剂量组创面微血管密度、VEGF、PI3K、Akt蛋白表达量高于低剂量组,mTOR蛋白表达量低于低剂量组。结论高剂量芦荟凝胶多糖可通过调控PI3K-Akt-mTOR信号通路促进急性放射性皮肤损伤模型创面新生血管形成,加快创面修复。     

Morphological analysis of three-dimensionally reconstructed frontal sinuses from Chinese Han population using computed tomography

International Journal of Legal Medicine - The uniqueness and reliability of frontal sinuses for personal identification have gained wide recognition in forensics. However, few studies have assessed...     

临时冠塑形对前牙种植美学的临床观察

目的 在前牙区通过利用临时冠对种植体周围软组织进行塑形,观察最终修复的美学效果及软组织的稳定性。方法 选取本院种植科21例上前牙需要种植病例,牙种植后即刻或延期行临时冠修复,对种植体周围软组织进行塑形,待软组织稳定后,行个性化转移杆取模,制作最终修复体,使用红色美学指数（PES）来评价种植体周围软组织的美学效果。结果 11例患者PES≥12,9例8≤PES≤11,获得较好的美学效果。1例患者前牙区种植体支持固定桥修复,由于牙槽骨水平吸收,牙龈退缩,后期通过牙龈瓷进行美学处理。21例患者最终修复后观察1年,通过PES再次评价,同样获得较好的美学效果。结论 前牙种植术后利用临时冠对软组织进行塑形,能够将软组织调整到较好的美观外形,并且形态较稳定。此方法在前牙区种植修复是必要的,值得在临床中广泛推广。     

Endothelial progenitor cells: the promise of cell-based therapies for acute lung injury

Background

Endothelial progenitor cells (EPCs) are defined as a special type of stem cell that have been found to directly incorporate into injured vessels and that participate in angiogenesis and reconstruction by differentiation into endothelial cells. EPCs are widely used to therapeutically treat cardiovascular disease, limb ischemia and vascular repair. However, the role of EPCs in inflammatory diseases, especially in lung injury, is less studied.

Objective

To investigate the application of EPCs to vascular repair, and the role of EPCs in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).

Methods

A computer-based online search was performed in the PubMed database and Web of Science database for articles published, concerning EPCs, angiogenesis, ALI/ARDS and stem cell transplantation

Conclusion

EPCs have a therapeutic potential for vascular regeneration and may emerge as novel strategy for the diseases that are associated with ALI/ARDS.