靶控输注依托咪酯在住院老年患者结肠镜下治疗的麻醉效果

目的观察靶控输注依托咪酯麻醉在住院老年患者结肠镜下治疗的麻醉效果及不良反应。方法选择192例患者,随机分成两组。靶控输注依托咪酯组(E组)、靶控输注丙泊酚组(P组),在BIS指导下施行麻醉,记录麻醉前、肠镜治疗中、麻醉后几个时段的BP、ECG、SpO2的变化。观察注射疼痛、肌阵挛、血压降低、心律失常、低氧饱和度、术后呕吐发生情况。结果 P组在BIS值降到50时的SBP、DBP、HR及在完全退镜时的DBP比用药前显著降低(P<0.05);E组生命征变化不明显(P>0.05);E组在BIS值降到50时的SBP、DBP及完全退镜时的SBP与P组比较差异有统计学意义(P<0.01)。两组患者麻醉起效时间、苏醒时间及手术持续时间比较差异均无统计学意义(P>0.05)。需要处理的低氧饱和度(SpO2<90%),E组低于P组(P<0.01);需要使用血管活性药(SBP<90mmHg或HR<50次/分),E组低于P组(P<0.01)。结论靶控输注依托咪酯麻醉应用于住院老年患者的结肠镜下治疗对心肺抑制小,麻醉效果满意。     

Postnatal development of multivesicular nuclear body in the Shiba goat Sertoli cell: an ultrastructural study

The multivesicular nuclear body (MNB) within the Sertoli cell nucleolus has been observed in the ruminant testis, but not in other mammalian species. Generally, the MNB is composed of vesicles, tubules and ribosome-like structures. This study has been conducted in order to clarify MNB formation during postnatal development. The testes were obtained from immature Shiba goats at 1, 2, 3, 4, and 5 months old, and from adults. They were fixed with 5% glutaraldehyde, postfixed with 1% OsO4, dehydrated in ethanol and embedded in Araldite-M. The serial cross-sections of seminiferous tubules were morphologically and morphometrically observed using light and transmission electron microscopy. In these Shiba goat testes, the MNB contained vesicles and tubules in various sizes, as well as ribosome-like structures. The volume of each Sertoli cell nucleus at each age (1, 2, 3, 4, 5 months old and adult) was about 269.3 microm3, 327.1 microm3, 361.3 microm3, 431.2 microm3, 525.0 microm3, and 760.4 microm3, respectively. The average number of vesicles per Sertoli cell nucleus was 0, 7.4, 11.1, 12.3, 15.5, and 32.7, respectively. At 1 month old, one or more nucleoli with fibrillar components were identified in the Sertoli cell nucleus. No MNB was observed. At 2 months old, a MNB first appeared, though it was underdeveloped and infrequently encountered. At this stage, a MNB, consisting of a small amount of vesicles and ribosomes, was located in the peripheral region of the nucleus. At later stages (3, 4, and 5 months old), MNBs gradually developed, increased in number, moved from the periphery to the central region of the nucleus, and associated with the nucleus to form a well-developed MNB. In the adults, the Sertoli cell nucleus displayed a well-developed and large-sized MNB situated in the central region.     

Polymorphisms of the dopamine metabolic and signaling pathways are associated with susceptibility to motor levodopa-induced complications (MLIC) in Parkinson’s disease: a systematic review and meta-analysis

Neurological Sciences - Dopamine replacement therapy remains the gold standard for symptomatic management of Parkinson’s disease worldwide. However, most patients will develop debilitating...     

大鼠坐骨神经固有频率测定

目的:确定实验大鼠坐骨神经的固有频率,观察不同状态下神经固有频率的变化,为神经再生修复研究中康复治疗仪器的频率选择提供参考。方法:将18只SD大鼠随机分为正常组、SNI 7 d组、SNI 28 d组,每组各6只,正常组不予干预,模型组行坐骨神经钳夹伤术。于造模后第7天通过振动平台对正常组、正常组的离体神经（即离体组）、SNI 7 d组,以及造模后第28天对SNI 28 d组大鼠施加振动频率源,在振动响应检测模块测得的数据基础上,根据频率特性曲线确定大鼠坐骨神经固有频率。结果:正常组、离体组、SNI 7 d组、SNI 28 d组大鼠坐骨神经频率特异性曲线均显示单一峰值;组间固有频率比较差异均无统计学意义（P=0.156）;组间两两比较显示,正常组与离体组、SNI 7 d组、SNI 28 d组比较,SNI 7 d组与SNI 28 d组比较,差异均无统计学意义（P>0.05）。固有频率检测值总体均数为9.635 Hz。结论:实验大鼠坐骨神经固有频率约为9.635 Hz,且固有频率是相对稳定的,不会因其所处状态不同而发生较大变化。     

广东省登革热流行与伊蚊种群随气候因素变化的相互关系

为探索登革热发病与伊蚊种群动态变化和气候因素的关系 ,为登革热的防治和监测提供科学依据 ,收集广东省 1990～ 2 0 0 1年的登革热发病资料 ,及潮州市 1995～ 2 0 0 1年伊蚊媒介监测资料及同期的平均气温、最低气温、最高气温、日照时间、降雨量、相对湿度等气象资料 ,用相关分析和多元逐步回归分析的方法进行分析。结果显示 ,登革热发病的Logistic回归方程 :p(1) =1 [1+e- (- 7 750 +0 391BI) ]。与布雷图指数 (breteauindex,BI)有关的气象因素是 :降雨量 (X2 )、日照时间、降雨天数、平均气温 (X1 )、最低平均气温、相对湿度(X3) ;回归分析得到回归方程 :^YBI=2 4 80 0 +0 82 6X1 +0 0 2 0X2 - 0 418X3。结论 :气候因素对伊蚊媒介密度的影响是复杂的 ,但其主要影响因素是最低平均气温、降雨量、相对湿度 ;影响登革热发病的主要因素是布雷图指数 (BI)。     

Multivesicular nuclear body in sertoli cells of the lesser mouse deer, Tragulus javanicus

The Sertoli cell of the lesser mouse deer, Tragulus javanicus, was examined using light and transmission electron microscopy. Similar to other ruminants, a multivesicular nuclear body (MNB) and laminated smooth endoplasmic reticulum (sER) were observed in the lesser mouse deer Sertoli cell. The MNB was present within the Sertoli cell nucleus, and consisted of vesicles, irregular tubules and ribosome-like structures. It was infrequent in the lesser mouse deer, which differs from domestic ruminants. Vesicles and irregular tubules seem to contain some materials with low and/or middle electron density, and be surrounded by electron dense materials. The diameter of vesicles was between 30 nm and 180 nm. Since the MNB, though less developed compared to that of bulls and goats, was present even in the Sertoli cell nucleus of the primitive ruminant-lesser mouse deer, it should be a common structure of ruminant Sertoli cells.