铬对克山病病区粮致心肌损伤的保护机理探讨

方法：采用克山病病区粮分别加Ｃｒ３＋、加Ｓｅ喂养大鼠，以荧光偏振法测定心肌细胞线粒体膜流动性，低温顺磁共振技术直接测定心、肝自由基含量，同时观察ＧＳＨ－Ｐｘ及ＳＯＤ活性变化。结果：在缺Ｓｅ状态下膜流动性降低，心、肝自由基水平升高，ＧＳＨ－Ｐｘ及ＳＯＤ活性降低，加Ｃｒ３＋或加Ｓｅ喂养的大鼠膜流动性明显改善，自由基水平显著降低，ＧＳＨ－Ｐｘ及ＳＯＤ活性亦有改善。结论：增强抗氧化功能可能是Ｃｒ３＋抗心肌损伤的重要机理之一。     

Acute fasting decreases the expression of GLUT1 and glucose utilisation involved in mouse oocyte maturation and cumulus cell expansion

Acute fasting impairs meiotic resumption and glucose consumption in mouse cumulus cell and oocyte complexes (COCs). This study examines the effects of acute fasting on the regulation of glucose transporter 1 (GLUT1) expression and glucose consumption in oocyte maturation. Our results indicate that the restriction of glucose utilisation by 2-deoxyglucose (2-DG) mimicked the inhibitory effects of acute fasting on oocyte meiotic resumption and cumulus cell expansion, effects that were rescued by high glucose concentrations in the culture medium. GLUT1 protein levels were higher in cumulus cells compared with oocytes, and GLUT1 expression in COCs increased with FSH treatment in vitro. However, under acute fasting conditions, GLUT1 expression in COCs decreased and the response to FSH disappeared. Exposure to high glucose conditions (27.5mM and 55mM), significantly increased both glucose consumption and GLUT1 levels in COCs. Inhibition of GLUT1 function using an anti-GLUT1 antibody significantly inhibited FSH-induced oocyte meiotic resumption. Taken together, these results suggest that acute fasting decreases GLUT1 expression and glucose utilisation, inhibiting the processes of oocyte maturation and cumulus cell expansion.     

徐汇区学龄前儿童早餐与体格发育的关系

为探讨儿童早餐与体格发育的关系，对上海市徐汇区学龄前儿童早餐与体格发育进行抽样调查。结果表明：早餐热能、蛋白质摄入不足的儿童．体重和县高均低于早餐热能、蛋白质摄入正常儿童。早餐营养素配比合理儿童的平均体重和身高高于总体平均值，而配比极不合理儿童的平均体重和身高较低。提示早餐可能影响学龄前儿童的体格发育，应引起社会和家庭的重视。     

CCTβ在大鼠脑内的分布及在昆虫细胞中的表达

目的:考察大鼠CTP:磷酸胆碱胞苷转移酶β(CCTβ)在脑内的分布及在昆虫细胞中的表达以研究其活性和特征.方法:用地高辛标记CCTβ探针对大鼠脑切片进行原位杂交;利用杆状病毒表达系统在粉纹夜蛾细胞中表达CCTβ;利用酶测活实验和代谢标记实验,研究表达蛋白的活性和特征,及对细胞磷脂酰胆碱(PC)合成的影响.结果:(1)CCTβ在海马的齿状回、CA1、CA2和CA4区有明显分布.(2)Tn细胞中表达的CCTβ含量是天然脑内的1×10~4倍,它的表达加快了细胞的PC合成.(3)软脂酰磷酸胆碱以及一些离子会抑制CCTβ活性;dCTP是CCTβ另一最适底物.结论:CCTβ在大鼠脑内有与精氨酸加压素片段(4—8)相似的分布.     

中西医结合治疗小儿支气管哮喘急性发作期90例

目的观察中西医结合治疗小儿支气管哮喘的疗效。方法治疗组病例采用中药宣郁汤雾化吸入并结合西药抗菌抗病毒治疗;对照组采用西药抗菌抗病毒及沙丁胺醇雾化吸入。结果治疗组90例,总有效率96.7%,对照组总有效率88.3%(P<0.05)。结论采用中西医结合治疗小儿支气管哮喘疗效较好。     

Inhibitory effects of magnolol on distal colon of guinea pig in vitro

The influence of plant product magnolol (0-100 microM) on the contractile activity of isolated colonic muscle strips in guinea pig and related mechanism were investigated. Magnolol did not affect the base tone of colon muscle strips, but it dose-dependently inhibited 40 mM KCl-, 1 microM carbachol (CCh)- and 10 microM serotonin (5-HT)-induced contractions at concentrations higher than 10 microM. And also, magnolol inhibited the 5-HT- or CCh-induced muscle contraction in calcium-free buffer. Furthermore, magnolol inhibited the KCl-induced contraction under the condition of procaine. In addition, inhibition rate of nifedipine plus magnolol on muscle strips was lower than that of nifedipine alone. Moreover, magnolol dose-dependently decreased the velocity of pellet propulsion in the concentration range of 0.1-10 microM, and totally inhibited pellet propulsion at the concentration higher than 30 microM. Thus, it can be concluded that magnolol may 1) block receptor-operated cation channels and the voltage dependent Ca2+ channel, and 2) inhibit calcium release from the sarcolemmal membrane (SR) through blocking InsP3-sensitive and ryanodine-sensitive pathways. This explains, at least partially, that Cortex magnoliae officinalis exerts therapeutic effects on gastrointestinal disease through relaxation of GI tract smooth muscles.     

对医院实施医药分开管理和分别核算的分析

笔者在较详细介绍医药分开管理和分别核算的政策规定,上海实行总量控制、结构调整政策,药品采购纳入政府采购序列,药品价格管理,医院药品受益实行集中上缴、适当返还的政策,阻断片面追求药的收益的职工收入分配改革方案等方面进行了分析,并在此基础上,提出了相应的对策,即有必要组建专门机构,制定工作计划,定期召开会议分析相关政策的执行情况,研究完善管理措施等     

有机药物中微量硒的荧光测定法

用荧光分光光度法测定了六种有机药物中的微量硒。样品用氧瓶燃烧法破坏,利用2,3-二氨基萘和硒(Ⅳ)的络合反应,用荧光分光光度计测定其荧光强度。本法的最低检出限为0.0476μg,平均回收率为100.0±3.0%(置信率95%),变异系数小于6%。含硒量在0～2μg/5ml符合线性关系。本法灵敏度高,样品用量少,误差较小,可满足有机药物中微量硒测定的要求。