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991.
992.
Effects of rheumatoid arthritis on employment and social participation during the first years of disease in The Netherlands 总被引:8,自引:1,他引:8
van Jaarsveld CH; Jacobs JW; Schrijvers AJ; van Albada-Kuipers GA; Hofman DM; Bijlsma JW 《Rheumatology (Oxford, England)》1998,37(8):848-853
OBJECTIVE: To study the effect of rheumatoid arthritis (RA) on working
capabilities and social participation, including non-paying jobs, during
the first 6 yr of disease. DESIGN: Cross-sectional study. METHODS: In April
1996, a self-reporting questionnaire was sent to 424 participants of a
population-based clinical trial of therapeutic strategies for early RA
initiated in 1990. RESULTS: A total of 363 completed questionnaires were
returned (response = 86%). Disease duration varied from < 1 to 6 yr
(mean 2.8 yr). The employment rate was low in the RA population compared to
the Dutch population. In the male 45- to 64-yr-old group, 63% of RA
patients were not employed compared to 32% of the Dutch population (P <
0.01). In the female 45- to 64-yr- old group, 76% of the RA population vs
67% of the Dutch were not employed (P < 0.05). Of the employed patients,
59% reported that RA affected their working capabilities, e.g. they worked
an average of 21 h per week less due to RA. Of the patients without a
paying job, 41% believed that this was (partly) due to RA. In addition,
fewer RA patients had non-paying jobs and they performed fewer household
activities compared to the general Dutch population. CONCLUSION: RA already
has a negative influence on the working capabilities, social participation
and household activities of these patients during the first 6 yr of
disease.
相似文献
993.
Expression of 5'-nucleotidase (CD73) related to other differentiation antigens in leukemias of B-cell lineage 总被引:1,自引:1,他引:1
Pieters R; Thompson LF; Broekema GJ; Huismans DR; Peters GJ; Pals ST; Horst E; Hahlen K; Veerman AJ 《Blood》1991,78(2):488-492
Ecto-5'nucleotidase (5'NT; CD73) expression was studied with a monoclonal antibody (7G2) and a radiochemical assay and compared with the expression of other antigens in B-cell-lineage leukemias on cells from 100 leukemic patients and two cell lines. A B-cell origin was confirmed by the expression of CD19 and HLA-DR. Four stages of B-cell leukemias were defined: stage I (pro-B) as CD10-, cytoplasmic mu- (c mu- ), surface Ig- (sIg-); stage II (cALL) as CD10+/c mu-/sIg-; stage III (pre-B) as CD10+ or -/c mu+/sIg-; and stage IV (B) as CD10-/c mu-/sIg+. A linear correlation was found between immunohistochemical and radiochemical determination of 5'NT (r = .86). 5'NT expression was low in T-cell leukemias and stage I, high in stages II and III, and low again in stage IV of B-cell leukemias. 5'NT expression was not related to c mu, CD20, CD21, CD22, CD34, and terminal deoxynucleotidyl transferase (TdT) expression, but was significantly related to CD10 and inversely related to kappa/lambda expression. However, the 5'NT activity in CD10+ leukemias (stages II and III) shows a very wide range. Within the group of CD10+ leukemias no differences were detected between 5'NT+ and 5'NT- cells in their expression of other B-cell antigens. We conclude that the place of 5'NT in leukemias corresponding to early stages of B-cell development has been characterized. 5'NT is expressed in CD10+ stages and decreases before the expression of sIgs. Future studies should make clear whether a high expression of this enzyme in CD10+ stages is a normal maturation phenomenon or a malignant phenomenon. 相似文献
994.
995.
Soft tissue angiofibroma: Clinicopathologic,immunohistochemical and molecular analysis of 14 cases
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Elise M Bekers Patricia JTA Groenen Marian AJ Verdijk Winny L Raaijmakers‐van Geloof Paul Roepman Robert Vink Nathalie DB Gilhuijs Joost M van Gorp Judith VMG Bovée David H Creytens Adrienne M Flanagan Albert JH Suurmeijer Thomas Mentzel Elsa Arbajian Uta Flucke 《Genes, chromosomes & cancer》2017,56(10):750-757
996.
997.
Nonmotor symptoms in healthy Ashkenazi Jewish carriers of the G2019S mutation in the LRRK2 gene
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![点击此处可从《Movement disorders》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Anat Mirelman PhD Roy N. Alcalay MD MSc Rachel Saunders‐Pullman MD Kira Yasinovsky BSc Avner Thaler MD Tanya Gurevich MD Helen Mejia‐Santana MS Deborah Raymond MS Mali Gana‐Weisz PhD Anat Bar‐Shira PhD Laurie Ozelius PhD Lorraine Clark PhD Avi Orr‐Urtreger MD PhD Susan Bressman MD Karen Marder MD MPH Nir Giladi MD the LRRK AJ consortium 《Movement disorders》2015,30(7):981-986
998.
Chromosome banding studies on 60 children with acute lymphocytic leukemia (ALL), including "null," pre-B, B, and T cell phenotypes, were performed. In 4 of 17 patients with pre-B cell ALL, we noted a previously undescribed chromosome translocation, t(1;19)(q23;q13). This translocation was not found in patients with "null" cell, B cell, or T cell ALL. Since each patient with the 1;19 translocation experienced early treatment failure, t(1;19)(q23;q13) may mark a subgroup of patients with pre-B cell ALL who have an especially poor prognosis. 相似文献
999.
BACKGROUND & AIMS: Enteric neurons can be characterized by their chemical coding, projections, and morphology. The aim of this study was to describe the different classes of human colonic circular muscle motor neurons. METHODS: Human colonic circular muscle motor neurons were identified by retrograde tracing with 1,1'-didodecyl 3,3,3',3'- indocarbocyanine perchlorate (Dil) applied to the circular muscle layer. Whole-mount preparations of the myenteric plexus were then double-labeled with antisera to choline acetyltransferase (ChAT) and/or nitric oxide synthase (NOS), or NOS and vasoactive intestinal peptide (VIP), and the position and immunoreactivity of Dil-filled neurons were recorded. RESULTS: Fifty-two percent of all Dil-filled neurons were ChAT immunoreactive, and 86% of these projected up to 11 mm orally, with 14% projecting short distances anally. Forty-eight percent of the Dil-filled neurons were NOS immunoreactive, and 77% of these projected up to 19 mm anally, with 23% projecting no more than 6 mm orally. A subpopulation of these NOS-immunoreactive motor neurons were also VIP- immunoreactive. A small population of myenteric neurons was immunoreactive for both ChAT and NOS, but none projected to the circular muscle. NOS-immunoreactive motor neurons projected for longer distances than those with ChAT immunoreactivity and were larger. CONCLUSIONS: There are two classes of human colonic motor neurons: one is excitatory (ChAT-immunoreactive) and mainly projects orally and the other is inhibitory (NOS +/- VIP immunoreactive) and projects preferentially anally. (Gastroenterology 1997 Dec;113(6):1916-23) 相似文献
1000.
Ligation of CD69 induces apoptosis and cell death in human eosinophils cultured with granulocyte-macrophage colony-stimulating factor 总被引:3,自引:1,他引:3
Peripheral blood (PB) eosinophils rapidly undergo apoptosis and cell death in vitro unless cultured in the presence of cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) in which their survival is prolonged for up to 10 days. CD69 is a type II membrane antigen expressed by cytokine-activated, but not freshly isolated, PB human eosinophils. We have examined the effect of ligation of CD69 by specific monoclonal antibody (MoAb) on the viability of human eosinophils cultured with recombinant human (rh)GM-CSF. Eosinophils were purified by immunomagnetic selection and cultured with GM-CSF (10(-10) mol/L). Eighteen hours after the start of culture, a panel of CD69 MoAb or controls (anti-CR3 or isotype-matched control MoAb) were added. Viability was assessed by trypan blue exclusion and apoptosis by morphologic assessment, DNA laddering, and flow cytometric analysis of eosinophil red autofluorescence. Up to 50% of the eosinophils had undergone apoptosis 48 hours after addition of anti- CD69 MoAb compared with less than 10% apoptosis for CR3 or the isotype matched control. The majority of apoptotic eosinophils excluded trypan blue at 48 hours post CD69 ligation. More apoptotic eosinophils were observed at later time-points and this was associated with loss of viability. At 120 hours post-addition of the anti-CD69 MoAb MLR3, 24% +/- 10.6% eosinophils were viable compared with 84% +/- 3.4% for the CR3 control (P < .001). A F(ab)2 fragment of CD69 MoAb P8, also induced apoptosis in GM-CSF cultured eosinophils. A more rapid induction of eosinophil apoptosis was obtained with CD69 MoAb immobilized via their Fc portions on protein-A coated plastic 96 well plates. Ligation of CD69 or CR3 resulted in the release of comparable quantities of eosinophil peroxidase at 48 hours post-ligation. These levels of EPO were consistent with the viability of these cells at 48 hours as assessed by exclusion of trypan blue. Finally, a neutralizing MoAb to TGF beta 1 had no effect on CD69-dependent apoptosis induction nor were there detectable quantities of TGF beta 1 in supernatants from GM-CSF-- cultured eosinophils ligated with CD69 or control MoAb. These results suggest that eosinophils cultured with GM-CSF can be induced to undergo apoptosis as a result of cell signalling mediated by perturbation of CD69. This may represent an important physiologic mechanism for eosinophil removal in vivo. 相似文献