A Peptide-based Vector for Efficient Gene Transfer In Vitro and In Vivo

Finding suitable nonviral delivery vehicles for nucleic acid–based therapeutics is a landmark goal in gene therapy. Cell-penetrating peptides (CPPs) are one class of delivery vectors that has been exploited for this purpose. However, since CPPs use endocytosis to enter cells, a large fraction of peptides remain trapped in endosomes. We have previously reported that stearylation of amphipathic CPPs, such as transportan 10 (TP10), dramatically increases transfection of oligonucleotides in vitro partially by promoting endosomal escape. Therefore, we aimed to evaluate whether stearyl-TP10 could be used for the delivery of plasmids as well. Our results demonstrate that stearyl-TP10 forms stable nanoparticles with plasmids that efficiently enter different cell-types in a ubiquitous manner, including primary cells, resulting in significantly higher gene expression levels than when using stearyl-Arg9 or unmodified CPPs. In fact, the transfection efficacy of stearyl-TP10 almost reached the levels of Lipofectamine 2000 (LF2000), however, without any of the observed lipofection-associated toxicities. Most importantly, stearyl-TP10/plasmid nanoparticles are nonimmunogenic, mediate efficient gene delivery in vivo, when administrated intramuscularly (i.m.) or intradermally (i.d.) without any associated toxicity in mice.     

血清P53抗体与原发性肝癌病理分化程度的相关性研究

目的了解P53抗体在西藏地区肝癌患者中的分布状况（阳性率）,分析P53抗体滴度与肝癌病理分化程度的关系。从而推断P53基因突变与肝癌病理分化程度的关系。方法用ELISA法定量测定肝癌患者首诊时血清P53抗体的水平,并与肝癌病理分化程度进行单因素统计分析。结果通过对48例西藏地区原发性肝癌患者血清P53抗体阳性率29．2％（14／48）单因素分析显示,血清P53抗体滴度与病理分化程度低并与肿瘤病理分化程度低有关。结论西藏地区血清P53抗体滴度与肿癌病理分化程度低有关,提示西藏地区肝癌患者血清P53抗体滴度与肿瘤不良物理学行为有关。为AFP（甲胎蛋白）阴性的肝癌患者提供补充诊断依据,     

宫颈癌自然史模型转移概率的研究进展

宫颈癌位于世界女性恶性肿瘤死亡顺位第二位.008年,全球有近53万宫颈癌新发病例和27.5 万死亡病例,其中约85％发生在发展中国家[1].筛查可以大幅降低宫颈癌发病率和死亡率[2,3].常用的筛查方法有醋酸碘染色肉眼观察法、细胞学检查和人乳头瘤病毒(HPV)DNA检测,它们的成本和效果均不同[4].不同国家和地区的经济发展水平和卫生资源条件差异较大,需要通过对各种筛查方法进行全面详尽的卫生经济学评价,进而筛选出适宜当地实际情况的筛查技术[5].     

心理干预对复发性尖锐湿疣患者疗效的影响

目的:调查复发性尖锐湿疣患者心理健康状况,探讨心理干预对复发性尖锐湿疣患者疗效的影响。方法:采用汉密顿焦虑量表(HAMA)对复发性尖锐湿疣患者心理健康状况进行调查,同时采用心理干预+综合治疗和不加心理干预的综合治疗分别对两组复发性尖锐湿疣患者进行治疗并相互比较。结果:和健康人群相比,复发性尖锐湿疣患者在焦虑心境、紧张、害怕、失眠、记忆或注意障碍、抑郁心境和会谈时行为表现等方面评分明显增高,差异有显著统计学意义(P<0.01);给予心理干预的治疗组疗效在治疗2月和3月后明显优于对照组(P<0.05)。结论:复发性尖锐湿疣患者存在一定程度心理健康障碍,心理干预对预防尖锐湿疣的复发存在积极的作用。     

动脉切开取栓术治疗血栓闭塞性脉管炎

目的探讨动脉切开取栓术治疗血栓闭塞性脉管炎（TAO）的临床经验。方法对我院2000年3月至2010年3月期间121例TAO患者中的14例（14条肢体：上肢2例和下肢12例）行动脉切开取栓术,其中8例肢体行单纯动脉切开取栓术,6例行同期动脉切开取栓联合骨髓或外周血干细胞移植术。结果 14例肢体中,11例肢体取栓成功,3例取栓不满意。取栓成功患肢术后疼痛症状消失。成功取栓患者中7例获得随访,随访5个月至8.5年（平均4年）,其中4例间歇性跛行及静息痛症状消失,2例未戒烟患者病情加重（1例行膝下截肢）,1例已戒烟患者仍有间歇性跛行。3例远端取栓不成功的患者,1例失访,1例术后未戒烟,症状加重行膝下截肢术,另1例已戒烟并随访3年,间歇性跛行等症状明显改善。结论动脉切开取栓术是治疗TAO安全、有效的方法 。     

2007年重庆市与西藏林芝地区碘缺乏病高危地区病情调查结果对比分析

目的 调查重庆市与西藏林芝地区碘缺乏病高危地区病情现状,为制订预防控制方案提供依据.方法 2007年由国家统一制订方案并抽样,重庆市调查城口和巫溪县,西藏林芝地区调查林芝、波密、米林和朗县4个县;林芝县抽查5个乡,其余县抽查3个乡;每个乡抽1所乡小学和2所村小学,以触诊和B超法同时检查8～10岁儿童甲状腺,同时检测尿碘;每个乡抽取2个村,每个村检测30户居民家庭食用盐含碘量,同时检测30名育龄妇女尿碘;搜索克汀病病例.结果 调查居民碘盐,重庆市调查340户,碘盐覆盖率98.82%(336/340);林芝地区调查915户,碘盐覆盖率为66.34%(607/905),重庆市高于林芝地区(x2=139.56,P＜0.01).儿童甲状腺容积触诊和B超检查,重庆市儿童甲状腺肿大率分别为9.27%(89/960)和8.34%(61/731);林芝地区儿童甲状腺肿大率分别为7.80%(102/1308)和5.53%(69/1248),两地触诊法检查肿大率比较差异无统计学意义(x2=1.37,P＞0.05),B超法重庆市高于林芝地区(x2=5.51,P＜0.05);儿童尿碘中位数,重庆市为319.15μg/L,其中城口县为345.75μg/L,巫溪县为281.39 μg/L;林芝地区为189.81 μg/L,其中林芝县为207.81μg/L、波密县为161.12μg/L、米林县为131.83 μg/L、朗县为334.60μg/L;育龄妇女尿碘中位数重庆市为248.42μg/L,林芝地区为121.25μg/L,儿童和育龄妇女尿碘均以重庆市为高;重庆市和林芝地区均未发现新发克汀病病例.结论 重庆市和林芝地区碘缺乏病高危地区儿童甲状腺肿大率降至10%以下,未发现新发克汀病,防治成效显著.重庆市存在碘过量;西藏林芝县和朗县儿童存在碘过量,妇女存在碘不足,其原因有待进一步调查,波密和米林均存在碘不足.重庆市应降低食盐加碘量,林芝地区在进一步调查基础上调整补碘措施.

Abstract：

Objective To investigate iodine deficiency disorders(IDD) in Chongqing and Linzhi, and to provide scientific basis for IDD control and prevention. Methods According to the national program developed in 2007, investigation was conducted in Chengkou and Wuxi county in Chongqing municipality, and Linzhi, Bomi,Milin and Langxian county in Linzhi prefecture. Five towns were sampled in Linzhi county, and 3 in other counties.In each town, one township primary school and two village primary schools were selected to inspect thyroid by B ultrasound and palpation, and urinary iodine of children aged 8 to 10 years was tested in these schools. Meanwhile,2 villages were selected in each town for test of salt iodine level and urinary iodine of childbearing age women and search cretin cases. Results Three hundred and forty families in Chongqing and 915 families in Linzhi were investigated. The coverage of iodized salt in Chongqing was 98.82%(336/340), which was significantly higher than that in Linzhi[66.34%(607/905), x2 = 139.56, P ＜ 0.01]. Goiter rate of children in Chongqing was 9.27%(89/960) by palpation and 8.34% (61/731) by B ultrasound, while goiter rate of children in Linzhi was 7.80%(102/1308) by palpation and 5.53% (69/1248) by B ultrasound. The difference of goiter rate by palpation between Chongqing and Linzhi was not statistically significant (x2 = 1.37, P ＞ 0.05 ). But goiter rate of children by B ultrasound in Chongqing was higher than that in Linzhi (x2= 5.51, P ＜ 0.05). In Chongqing, the median urinary iodine was 319.15 μg/L, and 345.75 μg/L in Chengkou county and 281.39 μg/L in Wuxi county. In Linzhi prefecture, the median urinary iodine was 189.81 μg/L, and 207.81 μg/L in Linzhi county, 161.12 μg/L in Bomi county, 131.83 μg/L in Milin county and 334.60 μg/L in Langxian county. The median urinary iodine in childbearing women were 248.42 μg/L in Chongqing and 121.25 μg/L in Linzhi. The median urinary iodine in Chongqing both in children and women were higher than those in Linzhi. No new cretin case was found in these two areas. Conclusions Goiter rate in high risk areas of IDD in Chongqing and Linzhi has decreased to less than 10%.No new cretin case is found in these areas. It can be concluded that the work of control and prevention is effective.There is excess iodine in Chongqing. In Linzhi county and Langxian county, iodine is excess in children and deficient in women. Further investigation should be conducted to find out the reason. Population iodine is excess in Bomi and Milin counties. The concentration of salt iodine should be decreased in Chongqing. In Linzhi prefecture,adding iodine measures should be adjusted based on further investigation.     

温度和湿度对硫酰氟熏蒸灭鼠效果的影响

目的观察温度和相对湿度对硫酰氟熏蒸灭鼠效果的影响。方法试验在模拟熏蒸箱内进行,均匀设计实验方案,通过单因素和多因素分析判断温度、湿度和剂量对硫酰氟熏蒸灭鼠效果的影响。结果单因素分析显示硫酰氟剂量和温度影响灭鼠效果,剂量从0．750g／m＾3增至0．892g／m＾3,鼠死亡率从30％升至70％;不同湿度（25％485％）鼠死亡率差异无统计学意义;均匀设计多因素分析显示相对湿度、剂量与小鼠的死亡率有关,以小鼠的死亡率为应变量,温度、相对湿度和剂量为自变量,进行二次型回归分析,得回归方程：P=-0．00839H＋1．03677D。结论硫酰氟熏蒸灭鼠效果好,在实际工作中,应根据湿度和温度变化科学调整熏蒸时的投药剂量。     

穴位注射联合西药治疗周围性面神经麻痹76例

目的：观察采用穴位注射联合西药治疗周围性面神经麻痹76例的临床疗效,并加以分析。方法：将76例患者随机分为2组,治疗组42例与对照组34例,对照组行西药治疗,治疗组行穴位注射联合西药治疗,两组疗程均为20d。结果：治疗组较对照组疗效更显著,42例患者中27例痊愈,7例显效,5例有效,总有效率92．85％。结论：采用穴位注射联合西药治疗周围性面神经麻痹的临床疗效满意,值得临床推广应用。