Survivin在宫颈癌及癌前病变组织中的表达及意义

目的: 探讨Survivin原位杂交表达在宫颈癌及癌前病变诊断中的应用效果.方法: 选取45例宫颈癌患者病理组织为观察组,宫颈上皮内瘤变组织45例为对照1组,正常宫颈组织45例为对照2组.入试者均行Survivin原位杂交检测.结果: Survivin在对照2组、对照1组、观察组阳性率分别为2.2%、48.9%和84.4%,差异均有统计学意义(P<0.01).Survivin在宫颈癌Ⅰ期、Ⅱ期和Ⅲ期中的阳性率,在宫颈癌高分化组、中分化组及低分化组中的阳性率和在宫颈癌伴淋巴结转移组与不伴淋巴结转移组中阳性率差异均无统计学意义(P>0.05).结论: Suvrvini在宫颈癌及癌前病变的发生发展中发挥一定作用,可作为宫颈癌预后不良的标志之一.     

紫暗舌与舌血流关系的研究

目的探索紫暗舌与舌血流的关系.方法运用激光多普勒血流仪分别对56例紫暗舌及54例正常舌从舌面多部位测试舌血流灌注量及流速,同时进行对照分析. 结果紫暗舌组患者的舌血流灌注量明显低于正常舌组(P ＜0.01),而流速明显增快(P ＜0.01).舌体各部位(舌尖、舌中、舌边)之间的舌血流灌注量及流速参数接近,无统计学差异. 结论紫暗舌患者舌血流灌注量降低、流速增快.舌微循环障碍可能是紫暗舌形成的主要因素之一.     

Granulopoietic differentiation in AML blasts in culture

A newly described monoclonal antibody detected an antigen (My-1) that is expressed on the cell surface during normal granulopoietic differentiation. The kinetics of expression of My-1 and NASD chloracetate esterase were studied during the formation in culture of blast colonies and normal granulopoietic colonies. My-1-positive cells increased during blast colony formation in 6 of 9 experiments, while NASD esterase-positive cells decreased. In contrast, in normal granulopoiesis in culture both markers increased coherently. We suggest that components of granulopoietic differentiation programs are expressed abnormally during blast colony formation in culture.     

Phase II evaluation of diaziquone (CI-904, AZQ) in the treatment of human malignant glioma

Summary Diaziquone, a new alkylating agent which crosses the blood brain barrier, has shown a 20% response rate in phase II studies in heavily pretreated patients. We have treated 23 patients at our institution as part of a multicentric phase II European trial of diaziquone. All had histologically proven malignant glioma unequivocally progressing on CT scan. Prior therapy had consisted of surgical excision (13 patients), cobalt radiotherapy to CNS (13 patients), and chemotherapy with nitrosourea derivatives (11 patients). Six patients had no prior therapy. Median age was 42 years (range 22–69) and performance status was 3+ or better. They were treated with monthly courses of diaziquone 5.5 mg/m² I.V. (10 min.) × 5 days. Dosage adjustments were made according to leucocyte and platelet nadirs. Thrombocytopenia was the dose limiting toxicity. Very mild gastrointestinal toxicity was observed. One patient developed hemolytic anemia. One complete response (clinical and CT scan), 7 partial clinical responses (3: > 50%, 4: 25–50%), and 1 disease stabilization (<25%) were documented. The longest response has now lasted over 26 months. These preliminary results show that chemotherapy with diaziquone can achieve a response rate as high as 35% in malignant glioma even in patients previously treated with a chemotherapy regimen including a nitrosourea (four of the seven objective responses were seen in such patients). Diaziquone is well tolerated and deserves further study in the management of malignant glioma. Address for offprints: Dr J Maral, Medical Oncology Service, Hôpital de la Salpetriere, 75651 Paris Cedex 13, France     

HISTOLOGICAL AND BIOCHEMICAL CHANGES IN NEONATAL THYROID TISSUES

ABSTRACT: Etling, N. and Larroche, J. C. (Unité Inserm 30, Hôpital des Enfants Malades, Centre de Recherches biologiques néonatales, Hôpital de Port Royal, Paris, France). Histological and biochemical changes in neonatal thyroid tissues. Acta Paediatr Scand 64:315, 1975.–The thyroid tissues of 17 infants who died between 3 hours and 46 days after birth were studied by histological and biochemical techniques. The morphological aspect and the iodine content of these tissues are not related to the gestational age of the neonates, but they are related to the survival time, There are dramatic events early after birth: desquamation of the epithelium and absence of colloid, low iodine content of tissue extract (less than 1 g127-I per mg of protein) and low percentage of thyroglobulin (less than 10 %). 24 hours after birth, the vesicles fill with colloid and the epithelium is cuboidal; the iodine content of the protein increases (between 1 and 2 g 127I per mg protein) as well as the thyroglobulin percentage (around 20 %). One week after birth, there is a maximum of colloid and flat epithelium; the iodine content of the protein extract is much higher (more than 2 g 127I per mg protein) as is thyroglobulin percentage (up to 40 %). Our studies of thyroid tissues of neonates suggest that a leakage of colloid, iodine and thyroglobulin takes place in the perinatal period, this phenomenon being followed by their rapid repletion.     

The role of small bowel radiology in the diagnosis and management of Crohn's disease

A total of 50 children with Crohn's disease were examined by barium follow-through and colonoscopy with ileoscopy, to determine the value of small bowel radiology. Of these children, 40 (80%) had evidence of small bowel Crohn's disease on ileoscopy and/or barium follow-through. Twenty-two (44%) had disease confined to the terminal ileum. Radiology diagnosed disease proximal to the terminal ileum in 18 cases (36%), including 5 children in whom the terminal ileum was normal. Ileoscopy was not possible in nine patients (18%), six of whom had small bowel disease on barium follow-through. Colonic involvement, demonstrated in 34 (68%), was the sole site of disease in 6 (12%). Fifteen (30%) children had surgery, which in six (12%) was determined by the radiological findings of complicated small bowel disease. As the terminal ileum may be uninvolved in the presence of proximal ileal disease, normal ileoscopy does not exclude small bowel Crohn's disease. Small bowel radiology remains necessary to assess the full extent of Crohn's disease in children.