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排序方式: 共有454条查询结果,搜索用时 31 毫秒
151.
152.
Mariana F.A. Funari Juliana S. de Barros Lucas S. Santana Antonio M. Lerario Bruna L. Freire Thais K. Homma Gabriela A. Vasques Berenice B. Mendonca Mirian Y. Nishi Alexander A.L. Jorge 《Clinical genetics》2019,96(3):261-265
Short stature homeobox (SHOX) haploinsufficiency is a frequent cause of short stature. Despite advances in sequencing technologies, the identification of SHOX mutations continues to be performed using standard methods, including multiplex ligation-dependent probe amplification (MLPA) followed by Sanger sequencing. We designed a targeted panel of genes associated with growth impairment, including SHOX genomic and enhancer regions, to improve the resolution of next-generation sequencing for SHOX analysis. We used two software packages, CONTRA and Nexus Copy Number, in addition to visual analysis to investigate the presence of copy number variants (CNVs). We evaluated 15 patients with previously known SHOX defects, including point mutations, deletions and a duplication, and 77 patients with idiopathic short stature (ISS). The panel was able to confirm all known defects in the validation analysis. During the prospective evaluation, we identified two new partial SHOX deletions (one detected only by visual analysis), including an intragenic deletion not detected by MLPA. Additionally, we were able to determine the breakpoints in four cases. Our results show that the designed panel can be used for the molecular investigation of patients with ISS, and it may even detect CNVs in SHOX and its enhancers, which may be present in a significant fraction of patients. 相似文献
153.
Kim HJ Barajas B Chan RC Nel AE 《The Journal of allergy and clinical immunology》2007,119(5):1225-1233
BACKGROUND: Dendritic cells (DCs) play a key role as antigen-presenting cells in the immune system. There is growing evidence that the redox equilibrium of these cells influences their ability to induce T-cell activation and to regulate the polarity of the immune response. This could affect the outcome of the immune response during systemic diseases and aging. OBJECTIVE: Our aim was to elucidate the mechanism by which the redox equilibrium of antigen-presenting DCs affects the delayed-type hypersensitivity (DTH) response during experimental modification of glutathione levels, as well as during aging. METHODS: We looked at the effect of glutathione depletion by diethyl maleate in DCs as well as during systemic administration on the DTH response to the contact-sensitizing antigens, oxazolone, and 2,4-dinitro-1-fluorobenzene. We also determined whether glutathione repletion with N-acetyl cysteine could influence the decline of the DTH response in aged mice. RESULTS: Glutathione depletion in bone marrow-derived DCs interfered in their ability to mount a DTH response on adoptive transfer into recipient mice. Glutathione depletion interfered in IL-12 production and costimulatory receptor expression in DCs, leading to decreased IFN-gamma production in the skin of recipient mice. Systemic diethyl maleate treatment exerted similar effects on the DTH response and IFN-gamma production, whereas N-acetyl cysteine administration reversed the decline of the DTH response in aged animals. CONCLUSION: Glutathione depletion downregulates T(H)1 immunity through a perturbation of DC maturation and IL-12 production. CLINICAL IMPLICATIONS: These data show that the induction of oxidative stress in the immune system, under disease conditions and aging, interferes in T(H)1 immunity. 相似文献
154.
Elena Larrieta-Carrasco Yvonne N. Flores Luis R. Macías-Kauffer Paula Ramírez-Palacios Manuel Quiterio Eric G. Ramírez-Salazar Paola León-Mimila Berenice Rivera-Paredez Guillermo Cabrera-Álvarez Samuel Canizales-Quinteros Zuo-Feng Zhang Tania V. López-Pérez Jorge Salmerón Rafael Velázquez-Cruz 《Experimental and molecular pathology》2018,104(1):50-58
Non-alcoholic fatty liver disease (NAFLD) is the accumulation of extra fat in liver cells not caused by alcohol. Elevated transaminase levels are common indicators of liver disease, including NAFLD. Previously, we demonstrated that PNPLA3 (rs738409), LYPLAL1 (rs12137855), PPP1R3B (rs4240624), and GCKR (rs780094) are associated with elevated transaminase levels in overweight/obese Mexican adults. We investigated the association between 288 SNPs identified in genome-wide association studies and risk of elevated transaminase levels in an admixed Mexican-Mestizo sample of 178 cases of NAFLD and 454 healthy controls. The rs2896019, rs12483959, and rs3810622 SNPs in PNPLA3 and rs1227756 in COL13A1 were associated with elevated alanine aminotransferase (ALT, ≥ 40 IU/L). A polygenic risk score (PRS) based on six SNPs in the ADIPOQ, COL13A1, PNPLA3, and SAMM50 genes was also associated with elevated ALT. Individuals carrying 9–12 risk alleles had 65.8% and 48.5% higher ALT and aspartate aminotransferase (AST) levels, respectively, than those with 1–4 risk alleles. The PRS showed the greatest risk of elevated ALT levels, with a higher level of significance than the individual variants. Our findings suggest a significant association between variants in COL13A1, ADIPOQ, SAMM50, and PNPLA3, and risk of NAFLD/elevated transaminase levels in Mexican adults with an admixed ancestry. This is the first study to examine high-density single nucleotide screening for genetic variations in a Mexican-Mestizo population. The extent of the effect of these variations on the development and progression of NAFLD in Latino populations requires further analysis. 相似文献
155.
Priscilla Davidson Negraes Berenice Quinzani Jord?o Veronica Elisa Pimenta Vicentini Mário Sérgio Mantovani 《Toxicology in vitro》2004,18(1):147-149
Chlorophyllin, a sodium-copper salt derivative of chlorophyll-a and -b, was evaluated for antimutagenic activity against ethyl methane sulfonate by the hypoxanthin-guanine-phosphoribosyl transferase (HGPRT) assay. The results obtained suggest that this chlorophyllin can potentiate the mutagenicity of an alkylating agent which induces DNA damage. 相似文献
156.
Martínez-Nava Gabriela A. Méndez-Salazar Eder O. Vázquez-Mellado Janitzia Zamudio-Cuevas Yessica Francisco-Balderas Adriana Martínez-Flores Karina Fernández-Torres Javier Lozada-Pérez Carlos Guido-Gómora Dafne L. Martínez-Gómez Laura E. Jiménez-Gutiérrez Guadalupe E. Pineda Carlos Silveira Luis H. Sánchez-Chapul Laura Sánchez-Sánchez Roberto Camacho-Rea María del Carmen Martínez-Armenta Carlos Burguete-García Ana I. Orbe-Orihuela Citlalli Lagunas-Martínez Alfredo Palacios-González Berenice López-Reyes Alberto 《Clinical rheumatology》2023,42(1):203-214
Clinical Rheumatology - Persistent hyperuricemia is a key factor in gout; however, only 13.5% of hyperuricemic individuals manifest the disease. The gut microbiota could be one of the many factors... 相似文献
157.
158.
Helal I Reed B McFann K Yan XD Fick-Brosnahan GM Cadnapaphornchai M Schrier RW 《Clinical journal of the American Society of Nephrology》2011,6(10):2439-2443
Summary
Background and objectives
The purpose of this study was to determine whether glomerular hyperfiltration (GH) occurring early in autosomal dominant polycystic kidney disease (ADPKD) is indicative of more rapid disease progression in children.Design, setting, participants, & measurements
One hundred eighty children with ADPKD (ages 4 to 18 years) with normal renal function were examined by renal ultrasound. Renal volume was calculated using a standard formula for a modified ellipsoid. Creatinine clearance was calculated from serum creatinine and 24-hour urine creatinine. GH was defined as creatinine clearance ≥140 ml/min per 1.73 m2.Results
Thirty-two children had GH (mean age 11.4 ± 3.6 years) and 148 had normal renal function (mean age 10.8 ± 3.9 years). Patients with GH at baseline demonstrated an increased rate of total renal volume growth (β: rate of change = +19.3 ± 10.8 cm3/year) over 5 years compared with those without GH at baseline (β = −4.3 ± 7.7 cm3/year), P = 0.008. Those with GH at baseline experienced a faster decline in creatinine clearance in subsequent years (β = −5.0 ± 0.8 ml/min per 1.73 m2 per year) compared with those without GH at baseline (β = +1.0 ± 0.4 ml/min per 1.73 m2 per year), P < 0.0001.Conclusions
This study revealed that occurrence of GH in ADPKD children is associated with a significantly faster decline in renal function and higher rate of kidney enlargement over time. GH combined with the increased renal volume may therefore be used as an early marker for a more severe progression of ADPKD in children. 相似文献159.
HIV infection induces morphometrical changes on the oral (buccal mucosa and tongue) epithelial cells
Pompermayer AB Gil FB França BH Machado MÂ Trevilatto PC Fernandes A de Lima AA 《Current HIV research》2011,9(1):11-16
The aim of this study was to assess morphological and morphometrical alterations of oral squamous epithelial cells in type 1 HIV infected individuals. Oral smears were collected from tongue and buccal mucosa of 30 HIV infected (experimental) and 30 non-infected (control) individuals by liquid-based exfoliative cytology. The cells were morphologically analyzed and the nuclear area (NA), the cytoplasmic area (CA) and the nucleus-to-cytoplasm area ratio (NA/CA) were calculated. No morphological differences were found between the groups. The mean values of CA were decreased in tongue (P=.00006) and buccal mucosa (P=.00242) in HIV infected individual, while mean values of NA were increased (P=.00308 and .00095, respectively) in the same group. NA/CA ratio for experimental group was increased in both collected places, with P=.00001 (tongue) and P=.00000 (buccal mucosa). This study revealed that HIV infection was able to induce morphometrical changes on the oral epithelial cells. 相似文献
160.
Mariana Diupotex María Berenice Martínez‐Salazar Alma Reyna Escalona‐Montao Jaime Zamora‐Chimal Norma Salaiza‐Suazo Adriana Ruiz‐Remigio Abigail Roldn‐Salgado María Magdalena Aguirre‐García Santiago Martínez‐Calvillo Paul Gaytn Ingeborg Becker 《Parasite immunology》2019,41(2)
Parasites have been engineered to express fluorescent reporter proteins, yet the impact of red fluorescent proteins on Leishmania infections remains largely unknown. We analysed the infection outcome of Leishmania mexicana parasites engineered for the constitutive expression of mKate protein and evaluated their immunogenicity in BALB/c mice. Infection of BALB/c mice with mKate transfected L. mexicana (LmexmKate) parasites caused enlarged lesion sizes, leading to ulceration, and containing more parasites, as compared to LmexWT. The mKate protein showed immunogenic properties inducing antibody production against the mKate protein, as well as enhancing antibody production against the parasite. The augmented lesion sizes and ulcers, together with the more elevated antibody production, were related to an enhanced number of TNF‐α and IL‐1β producing cells in the infected tissues. We conclude that mKate red fluorescent protein is an immunogenic protein, capable of modifying disease evolution of L. mexicana. 相似文献