冠状动脉CTA对冠状动脉粥样硬化性心脏病高危人群的随访观察价值

目的 探讨冠状动脉CT血管成像（CCTA）对冠状动脉粥样硬化性心脏病（简称冠心病）高危险因素人群（简称高危人群）的随访观察价值。方法 选取于我院接受CCTA检查且空腹血糖、血脂（甘油三酯、总胆固醇、高密度脂蛋白及低密度脂蛋白）等生化指标及体质量指数有2项或2项以上异常的80例患者,经临床干预后,间隔2年再次行CCTA检查,同时行上述生化指标及体质量指数检测,根据两次CCTA检查结果分为稳定组和进展组,并对2组两次生化指标、体质量指数及其变化率进行统计学分析。结果 稳定组60例,进展组20例。进展组第1次与第2次检查高密度脂蛋白水平差异有统计学意义（t=2.64,P=0.02）;稳定组第2次检查体质量指数、血糖、甘油三酯、总胆固醇及低密度脂蛋白明显小于第1次检查（t=2.10、2.68、3.45、4.26、5.90,P=0.04、0.01、0.001、0.000 1、0.000 1）;进展组第2次检查甘油三酯（t=2.39,P=0.02）和低密度脂蛋白高于稳定组第2次检查（t=3.98,P<0.000 1）;进展组血糖变化率高于稳定组（t=-2.04,P=0.045）。结论 CCTA对于随访观察高危人群冠心病有积极的指导意义,通过加强对冠心病危险因素的积极干预,改变不良生活习惯,可以有效延缓或阻止冠心病的发生及发展。     

Reticulocytes. I. Isolation and in vitro maturation of synchronized populations

Studies of reticulocyte maturation have been limited by the inability to obtain pure populations of age-synchronized reticulocytes and by the absence of well-defined methods for the maturation of reticulocytes in vitro. Many of these problems were overcome using temporary suppression of erythropoiesis with thiamphenicol and phlebotomy resulting in a highly reproducible reticulocyte response, Percoll density gradient separation of cells yielding essentially pure populations of age- synchronized reticulocytes, and liquid culture techniques where cell lysis is minimal. The system allows reproducible study of well-defined cohorts of reticulocytes as they mature into erythrocytes. During in vitro maturation we serially monitored changes in reticulocyte count, glucose consumption, 125I-transferrin binding, fluorescein (FITC)- labeled transferrin binding, the activities of four erythrocyte enzymes (glucose-6-phosphate dehydrogenase, pyruvate kinase, phosphofructokinase, and lactate dehydrogenase) and the appearance of cells on scanning electron microscopy. These variables changed at different rates suggesting that multiple mechanisms underlie these maturational events. Transferrin binding and reticulocyte count decreased most rapidly and reached values near zero after three to four days in culture. The four enzyme activities decreased much more slowly, and only two reached pretreatment values after seven days in culture. In contrast to the findings of others, scanning electron microscopy suggested that cells do not assume the normal biconcave shape in this system. The methods described make it feasible to study the process of reticulocyte maturation in vitro. The data presented represent a first step in the study of the coordination and interrelationships of various maturational processes.     

Effects of chronotropic incompetence on exercise capacity in people with heart failure versus age-matched controls

Heart Failure Reviews - Chronotropic incompetence (CI) is an inability to adequately raise heart rate during physiological stress. We established CI prevalence and exercise capacity in heart...     

流行性出血热的CT表现(附38例分析)

目的:总结流行性出血热(EHF)的CT表现及临床应用价值。材料和方法:搜集38例经临床确诊并行SCT扫描的EHF,临床分型:重型11例,中型22例,轻型5例。对照临床对其CT表现进行分析和总结。结果:双肾明显肿胀26例,全部为中、重型;双肾无明显肿胀12例,其中包含5例轻型。38例EHF中肾包膜下出血2例,肾周积液10例,有腹水的为16例,胸腔积液16例,另外还有肺出血1例、肺水肿2例、肺不张1例、胰腺轻度肿胀2例。结论:通过对EHF病人进行CT检查,可快速准确了解相关脏器改变,及时发现病变,准确判断病情严重程度,以制定正确的治疗方案,赢得宝贵治疗时间,使影像学在EHF诊断方面发挥重要作用。     

扩张型心肌病患者两种治疗方法前后microRNA-126 和microRNA-30a 水平表达的研究

目的 探讨microRNA-126（miRNA-126） 和microRNA-30a(miRNA-30a) 检测在扩张型心肌病（dilatedcardiomyopathy,DCM）患者诊断及预后评估的价值。方法 收集2014.2 ～ 2019.3 期间40 例DCM 患者,按治疗方法分为手术治疗组20 例和一般治疗组20 例,同期健康体检者20 例为对照组,采用qRT-PCR 法?检测治疗前后患者血清中miRNA-126 和miRNA-30a 的相对含量,并进行统计学分析。结果 ① DCM 患者与对照组血清miRNA-126 相对表达量为[1.40(0.44,3.28) vs 1.15（0.44,2.25）],miRNA-30a 为[2.37(0.70,4.25) vs 1.05（0.48,2.20）],差异无统计学意义（Z=-0.557,-1.662,P ＞ 0.05）。②治疗后,一般治疗组miRNA-126 为 1.35(0.66,2.00),miRNA-30a 为1.2(0.77,4.55)。手术治疗组miRNA-126 为 0.16(0.10,0.26),miRNA-30a 为0.20(0.14,0.33), 手术治疗组治疗前后miRNA-126,miRNA-30a 表达量差异有统计学意义（均Z =-3.059,均P ＜ 0.05）。一般治疗及手术治疗两者表达量差异有统计学意义（miRNA-126:Z = -3.806,P ＜ 0.05;miRNA-30a:Z = -3.24,P ＜ 0.05）。③ miRNA-126 与NT-proBNP 呈负相关;循环miRNA-30a 与NT-proBNP 呈正相关,与射血分数呈负相关。④ miRNA-126 的12 个月相对危险度为 0.768（95%CI 0.431~1.37,P ＞ 0.05）,miRNA-30a 的12 个月生存分析其相对危险度为1.141（95% CI 0.775~1.678,P ＞ 0.05）。结论 miRNA-126 和miRNA-30a 可为两种不同治疗方法的评价提供一定的参考价值。