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31.
This paper examines temporal changes in the organisation of the cytoskeleton within isolated articular chondrocytes cultured
for up to 7 days in agarose constructs. Fluorescent labelling and confocal microscopy were employed to visualise microtubules
(MT), vimentin intermediate filaments (VIF) and actin microfilaments (AMF). To quantify the degree of cytoskeletal organisation
within populations of cells, a novel image analysis technique has been developed, and fully characterised. Organisation was
quantified in terms of an Edge Index, which reflects the density of ‘edges’ present within the confocal images as defined
by a Sobel digital filter. This parameter was shown to be independent of image intensity and, for all three cytoskeletal components,
was validated statistically against a visual assessment of organisation. Both MT and VIF exhibited fibrous networks extending
throughout the cytoplasm, while AMF appeared as punctate units associated with the cell membrane. The use of the Edge Index
parameter revealed statistical significant temporal variation, in particular associated with VIF and AMF. These findings indicate
the possibility of cytoskeletal mediated temporal variation in many aspects of cell behaviour following isolation from the
intact tissue. Furthermore, the image analysis techniques are likely to be useful for future studies aiming to quantify changes
in cytoskeletal organisation. 相似文献
32.
Bader N 《Business and health》1993,11(12):62-4, 66
33.
目的;探讨手术治疗胸椎结核的更好方法。方法;自1990年以来,对21例胸椎结核导致椎柱不稳的患者,应用病灶清除,哈氏棒内固定,椎间及椎板植骨的手术方法。本组平均33.2岁。胸7椎体6例,胸8椎体8例,胸10椎体7例,椎体压缩〈1/2椎体高度13例,〉1/2椎体高度8例,并不全瘫14例。手术中先行病灶清除,然后哈氏棒内固一,撑开后再次清除病灶,取肋骨和髂骨行椎间及椎板上植骨。术后化疗12~15月。结 相似文献
34.
Prednicarbate Versus Conventional Topical Glucocorticoids: Pharmacodynamic Characterization In Vitro
Lange Katharina Gysler Anja Bader Michael Kleuser Burkhard Korting Hans Christian Schäfer-Korting Monika 《Pharmaceutical research》1997,14(12):1744-1749
Purpose. Pharmacodynamic characterization of topical glucocorticoids as prednicarbate (PC), its metabolites prednisolone 17-ethylcarbonate (PEC) and prednisolone (PD), betamethasone 17-valerate (BMV), beta-methasone (BM) and desoximetasone (DM) by evaluating their effects on epidermal and dermal cells. Synopsis of pharmacokinetic and pharmacodynamic studies, possibly explaining the improved benefit-risk ratio of prednicarbate.
Methods. Isolated foreskin keratinocytes were used to investigate the influence on epidermal inflammatory processes, dermal fibroblasts of the same origin to study antiproliferative activities of glucocorticoids. Interleukins were measured by ELISA-assay, the influence on II-l-production also on mRNA-level by RNAse protection assay. Proliferation was assessed by 3H thymidine incorporation and biodegradation by HPLC/UV-absorption. Cell viability was controlled by MTT assay.
Results. In keratinocytes, inflammation was induced by TNF, resulting in an increased II- l synthesis. This cytokine was particularly suppressed by PC and BMV, whereas PEC, PD, DM and BM were less potent (p 0.05). Since, however, the double ester PC is rapidly degraded in keratinocytes, a RNAse-protection assay of II-1 mRNA was performed allowing short incubation times and thus minimizing biodegradation effects. In agreement with the previous experiment, the antiinflammatory potency of native PC was confirmed. In fibroblasts, II-l and II-6 synthesis indicate proliferation and inflammation respectively. Whereas PC inhibited II- l and II-6 production in fibroblasts to a minor extent only, it was strongly reduced by the conventional glucocorticoids and PEC (p 0.05). The minor unwanted effect of PC on fibroblasts was also reflected by its low influence on cell proliferation as assayed by 3H thymindine incorporation. More pronounced antiproliferative features were observed with BM, PEC and espectially BMV.
Conclusions. Correlating antiphlogistic effects in keratinocytes (suppression of II-l) with antiproliferative effects in fibroblasts (suppression of II-l and II-6), the improved benefit–risk ratio of PC compared to conventional glucocorticoids does not result only from distinct drug metabolism in the skin but also from a specific influence on the cytokine network. 相似文献
35.
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37.
Polydactyly may be preaxial or tibial (hallux-side), postaxial or ulnar (side of the little toe) and central (middle toes). The duplication may appear at the distal and medial phalanges or at the whole digit. The metatarsal bone may be part wise or completely duplicated, the accessory toes may share only one metatarsal. Surgical intervention may be indicated in shoe problems, for esthetic reasons or, especially in duplication of the metatarsales, because of secondary deviation of the toes and therefore shoe problems or plantar callosities. Preoperative analysis including x-ray is of great importance to achieve good functional and cosmetic results. 相似文献
38.
Tschöpe C Koch M Spillmann F Heringer-Walther S Mochmann HC Stauss H Bader M Unger T Schultheiss HP Walther T 《Immunopharmacology》1999,44(1-2):111-117
An increase in myocardial bradykinin (BK) might be a mechanism to protect the heart during acute myocardial infarction (MI). To characterize the regulation of the myocardial B2 receptor during MI, we studied the expression of this BK receptor in the right ventricle (RV), left ventricle (LV) and myocardial septum (S) 24 h after left coronary ligation. Experiments were performed in male Wistar Kyoto rats (n = 10) and compared with sham operated animals (n = 6). After total RNA extraction, the myocardial B2-receptor expression was analyzed by a RNase protection assay (n = 6), using a specific probe from the coding region of the receptor gene. After 24 h, rats with MI were normotensive and showed an impaired left ventricular function. The B2-receptor expression of the LV of these rats was significantly elevated (2.3-fold) compared to sham operated rats. Furthermore, we found a dramatic upregulation of the B2 receptor in the RV (7.8-fold) and a dramatic expression of B2 receptor mRNA in S of infarcted hearts, whereas in the S of sham operated rats no B2 receptor expression could be detected. Our data show clearly that the described increase in BK during myocardial ischemia is accompanied by an elevated B2-receptor expression in the infarcted and non-infarcted parts of cardiac ventricles. 相似文献
39.
The authors report a case that offers insight into the diagnostic challenges of "Munchausen Syndrome by Proxy." Initial presentation with history of fever and later with intractable vomiting led to invasive and expensive diagnostic evaluation before confirming the diagnosis. Certain toxic effects of emetine were clinically noted. Biochemical and clinical improvement were clearly demonstrated after withdrawal of the toxic agent. 相似文献
40.