全文获取类型
收费全文 | 1269篇 |
免费 | 93篇 |
国内免费 | 22篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 39篇 |
妇产科学 | 13篇 |
基础医学 | 151篇 |
口腔科学 | 45篇 |
临床医学 | 178篇 |
内科学 | 211篇 |
皮肤病学 | 6篇 |
神经病学 | 165篇 |
特种医学 | 166篇 |
外科学 | 104篇 |
综合类 | 24篇 |
预防医学 | 54篇 |
眼科学 | 12篇 |
药学 | 55篇 |
肿瘤学 | 159篇 |
出版年
2023年 | 7篇 |
2022年 | 7篇 |
2021年 | 17篇 |
2020年 | 8篇 |
2019年 | 14篇 |
2018年 | 27篇 |
2017年 | 21篇 |
2016年 | 18篇 |
2015年 | 22篇 |
2014年 | 26篇 |
2013年 | 45篇 |
2012年 | 53篇 |
2011年 | 57篇 |
2010年 | 48篇 |
2009年 | 75篇 |
2008年 | 48篇 |
2007年 | 47篇 |
2006年 | 55篇 |
2005年 | 34篇 |
2004年 | 34篇 |
2003年 | 40篇 |
2002年 | 43篇 |
2001年 | 35篇 |
2000年 | 37篇 |
1999年 | 27篇 |
1998年 | 55篇 |
1997年 | 44篇 |
1996年 | 44篇 |
1995年 | 47篇 |
1994年 | 27篇 |
1993年 | 32篇 |
1992年 | 18篇 |
1991年 | 12篇 |
1990年 | 19篇 |
1989年 | 34篇 |
1988年 | 33篇 |
1987年 | 20篇 |
1986年 | 14篇 |
1985年 | 20篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1982年 | 19篇 |
1981年 | 12篇 |
1980年 | 17篇 |
1978年 | 8篇 |
1977年 | 15篇 |
1976年 | 9篇 |
1973年 | 2篇 |
1904年 | 3篇 |
1897年 | 2篇 |
排序方式: 共有1384条查询结果,搜索用时 187 毫秒
61.
M Brigita Tan-Sindhunata Inge B Mathijssen Margriet Smit Frank Baas Johanna I de Vries J Patrick van der Voorn Irma Kluijt Marleen A Hagen Eveline W Blom Erik Sistermans Hanne Meijers-Heijboer Quinten Waisfisz Marjan M Weiss Alexander J Groffen 《European journal of human genetics : EJHG》2015,23(9):1151-1157
Fetal akinesia deformation sequence (FADS) refers to a clinically and genetically heterogeneous group of disorders with congenital malformations related to impaired fetal movement. FADS can result from mutations in CHRNG, CHRNA1, CHRND, DOK7 and RAPSN; however, these genes only account for a minority of cases. Here we identify MUSK as a novel cause of lethal FADS. Fourteen affected fetuses from a Dutch genetic isolate were traced back to common ancestors 11 generations ago. Homozygosity mapping in two fetuses revealed MUSK as a candidate gene. All tested cases carried an identical homozygous variant c.1724T>C; p.(Ile575Thr) in the intracellular domain of MUSK. The carrier frequency in the genetic isolate was 8%, exclusively found in heterozygous carriers. Consistent with the established role of MUSK as a tyrosine kinase that orchestrates neuromuscular synaptogenesis, the fetal myopathy was accompanied by impaired acetylcholine receptor clustering and reduced tyrosine kinase activity at motor nerve endings. A functional assay in myocytes derived from human fetuses confirmed that the variant blocks MUSK-dependent motor endplate formation. Taken together, the results strongly support a causal role of this founder mutation in MUSK, further expanding the gene set associated with FADS and offering new opportunities for prenatal genetic testing. 相似文献
62.
JK Gass SK Chan E Rytina DC Greenberg NP Burrows 《Journal of the European Academy of Dermatology and Venereology》2010,24(5):601-603
Background Merkel cell carcinoma (MCC) is a rare malignant cutaneous tumour, the incidence of which is increasing. Second malignancies have been reported to occur with high incidence in these patients. Objectives We report the rate and nature of multiple malignancies in patients with MCC treated over a 10 year period in Addenbrooke’s Hospital in Cambridge, United Kingdom, as well as the temporal relationship of these additional malignancies to the diagnosis of MCC. Results The 27 patients had an approximately equal sex incidence with a median age at diagnosis of 79 years. Seventy percent (n=19) of patients had a second primary malignant tumour; and 7 of these patients had two or more tumours in addition to the MCC. Eighteen patients had additional cutaneous malignancies: melanoma, squamous cell carcinoma and basal cell carcinoma, and 8 patients presented non‐cutaneous malignancy including colorectal, haematological and breast tumours. Of the 28 additional tumours in our patients, half were diagnosed prior to presentation of MCC, 32% within 6 months of diagnosis, and 18% between 6 months and 3 years after diagnosis. Possible reasons for the high rate of additional tumours in this population are discussed. Conclusions Our figures reflect a higher incidence of multiple malignancies in those with Merkel cell tumour than has previously been reported. This has important implications for the care and surveillance of these patients. 相似文献
63.
64.
Thomas Jakobsen Jørgen Baas Joan E. Bechtold Brian Elmengaard Kjeld Søballe 《Clinical orthopaedics and related research》2010,468(3):867-874
Background
Long-term survival of uncemented total joint replacements relies on osseointegration. With reduced bone stock impacted morselized allograft enhances early implant fixation but is subject to resorption. 相似文献65.
66.
67.
DC Wilson MJ Cunningham MMcC Reid SS Johnston TF Fannin 《Acta paediatrica (Oslo, Norway : 1992)》1992,81(1):84-85
A baby with unilateral cleft lip, midline cleft palate and hypertelorism developed meningitis in the first 48 h of life. Examination of the nasopharynx showed a soft tissue mass, which was confirmed as a basal encephalocele by computed tomography. There was also congenital hydrocephalus and the corpus callosum was absent. Surgical treatment included repair of the anterior basal skull defect, repair of the lip and palate, and ventriculo-peritoneal shunt. There is currently evidence of developmental delay and right-sided visual impairment due to Morning Glory syndrome. This case demonstrates that basal encephalocele should be considered in any baby with midline facial deformity who develops meningitis. 相似文献
68.
E. W. Eijdems G. J. Zaman M. de Haas C. H. Versantvoort M. J. Flens R. J. Scheper E. Kamst P. Borst F. Baas 《British journal of cancer》1995,72(2):298-306
We have analysed the contribution of several parameters, e.g. drug accumulation, MDR1 P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP) and topoisomerase (topo) II, to drug resistance in a large set of drug-resistant variants of the human non-small-cell lung cancer cell line SW-1573 derived by selection with low concentrations of doxorubicin or vincristine. Selection with either drug nearly always resulted in MDR clones. The resistance of these clones could be explained by reduced drug accumulation and was associated with a decrease rather than an increase in the low MDR1 mRNA level. To test whether a decrease in MDR1 mRNA indirectly affected resistance in these cells, we introduced a MDR1-specific hammerhead ribozyme into wild-type SW-1573 cells. Although this led to a substantial reduction in MDR1 mRNA, it did not result in resistance. In all resistant clones we found an altered form of the multidrug resistance-associated protein (MRP), migrating slightly slower during SDS-polyacrylamide gel electrophoresis than MRP in parental cells. This altered MRP was also present in non-P-gp MDR somatic cell hybrids of the SW-1573 cells, demonstrating a clear linkage with the MDR phenotype. Treatment of crude cellular membrane fractions with N-glycanase, endoglycosidase H or neuraminidase showed that the altered migration of MRP on SDS-PAGE is due to a post-translational modification. There was no detectable difference in sialic acid content. In most but not all doxorubicin-selected clones, this MDR phenotype was accompanied by a reduction in topo II alpha mRNA level. No reduction was found in the clones selected with vincristine. We conclude from these results that selection of the SW-1573 cell line for low levels of doxorubicin or vincristine resistance, predominantly results in MDR with reduced drug accumulation associated with the presence of an altered MRP protein. This mechanism can be accompanied by other resistance mechanisms, such as reduced topo II alpha mRNA in case of doxorubicin selection. 相似文献
69.
Expression and distribution of id helix-loop-helix proteins in human astrocytic tumors 总被引:12,自引:0,他引:12
Vandeputte DA Troost D Leenstra S Ijlst-Keizers H Ramkema M Bosch DA Baas F Das NK Aronica E 《Glia》2002,38(4):329-338
The Id family of helix-loop-helix proteins is involved in a variety of processes, such as development, proliferation, and angiogenesis. In this study, we investigated the expression pattern of Id1, Id2, and Id3 in surgical specimens of human glial tumors. Western blot analysis demonstrated that all three Id proteins were expressed in astrocytic tumors. Expression levels in high-grade tumors were higher than in low-grade tumors. Immunohistochemical analysis confirmed that many of the tumor astrocytes exhibited strong Id1-3 IR. In contrast, in adult human normal brain, Id expression was low both in resting astrocytes and in endothelial cells. In tumor cells, Id proteins displayed cytoplasmic as well as nuclear localization. Id1-3 IR scores in tumor cells were positively correlated with proliferation indices. Moreover, Id1-3 IR was detected in endothelial cells of the astrocytic tumor blood vessels. The vascular Id1-3 expression correlated positively with tumor vascularity and grade. These results support the role of the Id gene family in the enhanced proliferative potential of tumor astrocytes. The evidence also supports the involvement of the Id gene family in tumor angiogenesis, a process that critically influences the malignant behavior of glial tumors. 相似文献