Synthesis of M Protein by Mouse Myeloma Tumor: Correlation of In Vitro and In Vivo Methods

1. In vitro incubation of Potter-Pilgrim No. 5563 and No. 5647 mousemyeloma tumors with tritiated-d,l-leucine produced demonstrable radioactivity in the respective myeloma proteins.

2. In vivo inoculation of H₃-d,l-leucine in tumor bearing animals showedsimilar incorporation and in addition showed incorporation of radioactivityinto albumin and alpha globulin.

3. It is suggested that the in vitro studies better serve to identify the isolatedtissue function in protein synthesis.

4. Mouse myeloma studies confirm previous results in human multiplemyeloma and indicate that in vitro incubation of protein producing tissueswith H₃-d,l-leucine can demonstrate protein synthesis.

Submitted on September 24, 1960 Accepted on June 6, 1961     

Morphine consumption and respiratory depression in children receiving postoperative analgesia from continuous morphine infusion or patient controlled analgesia

Thirty children, aged between five and 15 years, were randomly allocated to receive postoperative analgesia from continuous morphine infusion (CMI) or patient controlled analgesia (PCA), also using morphine. The children's morphine consumption, respiratory rates, oxygen saturations and observation points during which they were sleeping were recorded during two periods, one on the day of operation and one the following day. The median dose of morphine consumed by the children using PCA was significantly larger than that consumed by the children having continuous infusions. Children aged between nine and 15 years using PCA had significantly lower minimum respiratory rates and minimum oxygen saturations than similarly aged children receiving continuous infusions. There was no significant difference between the PCA and CMI groups in the number of observation times that the children were asleep or in the minimum respiratory rates and minimum oxygen saturations in the awake and sleeping children.     

碳糊电极溶出伏安法测定奋乃静等抗精神病药物

本文报道一种测定吩噻嗪类治疗精神病药物奋乃静、氟奋乃静、氯丙嗪和三氟拉嗪的灵敏电化学分析方法。该类药物在碳糊电极上预富集后,进行溶出伏安测定,溶出电流与浓度在一定范围内具有线性关系。最低检测限可达5ng/ml。用本方法可以不经分离,直接测定药片和人体液中上述药物的含量。作者提出了一种新型挤压式碳糊电极,对上述药物在该电极上的富集机理和测试条件进行了系统研究。认为药物的富集作用是由吸附和萃取引起的,并用计时库仑法定量地估价了这两部分在富集中所起的作用。