Induction of mixed-function oxidases in mouse liver by psoralens

8-methoxypsoralen, the most widely used psoralen in photochemotherapy, was shown to induce mixed-function oxidases. Induction of mixed-function oxidases in mouse liver was examined after mice were given a single dose of one of three psoralens. 8-methoxypsoralen clearly induced p-nitro-anisole-O-demethylase and slightly induced aryl hydrocarbon hydroxylase. Psoralen and 4,5′,8-trimethylpsoralen failed to induce either enzyme. These results represent the first enzyme induction studies on these clinically useful compounds. The studies may have relevance in understanding the clinical differences in skin photosensitizing activity and photochemotherapeutic effectiveness of the compounds in such diseases as psoriasis and vitiligo.     

SENSORY DEPRIVATION IN YOUNG CHILDREN

Summary The importance of the role of intentional movement and active exploration in the development of a child's knowledge concerning his body, his movement and his surrounding is recognized. Senoristasis is defined and the concept applied to deprivation studies with animal and human subjects. It is suggested that the principle can be used to explain behavioural patterns found in two groups of children who may suffer from sensory deprivation; namely those who live in environments which provide insufficient stimulation and those who lack the necessary mobility to explore the environment in which they live. The importance in a young child's development of a rich sensory environment and opportunities for exploration are stressed.     

ASSESSMENT OF FLEXOR TENOSYNOVITIS IN RHEUMATOID ARTHRITIS

Assessment of the effect of therapy—physical, surgical,or local corticosteroid Injections—in the management ofrheumatoid flexor tenosynovitis has proved difficult. This paperdescribes a simple, accurate and reproducible method of documentationusing a photocopying technique. Its application to a study ofthe role of hydrocortisone injections in the management of flexortenosynovitis is presented.     

The Intercellular Substances of Bone Marrow. I. Preliminary Studies of the Physical and Chemical Character of the Intercellular Bone Marrow Substances in Normal Rabbits

Separation of cellular and noncellular fractions of rabbit bone marrow wasaccomplished by mechanical disruption of the marrow stroma and centrifugation. Viscosity studies performed on the intercellular substances showed a decreasing viscosity with increasing age of the animals. Chemical analyses confirmed the presence of mucopolysaccharide material in the intercellular substance of the marrow, but neither this material nor the fat present in the stromacould account for the viscosity measured or the changes in viscosity observed.

Submitted on January 9, 1961 Accepted on February 23, 1961     

UREA AND GUANIDINE HYDROCHLORIDE INDUCED DISSOCIATION AND DENATURATION OF BACTERIOPHAGE F2 CAPSID

A single, low molecular weight protein is found after urea or guanidine hydrochloride (Gdn.HCl) treatment of empty capsids derived from bacteriophage f2. The final product of denaturation is apparently a monomer, existing as a random coil in ≥ 4.0 M Gdn.HCl but in a less extended form in 8.0 M urea. In contrast, an 11 S protein component is isolated after treatment of the intact virus with 4.0 M Gdn.HCl (Zelazo & Haschemeyer, 1969), indicating that RNA plays a role in stabilizing larger subunits. Denaturation by Gdn.HCl occurs in two stages as measured by changes in CD and Stokes radius: dissociation that involves a structural perturbation of aromatic side chains, followed by a major, cooperative transition that evidently results in the loss of all noncovalent structure. Denaturation by urea appears to be a much less cooperative process that occurs in several steps over a wide range of urea concentration (1–7 M). In both urea and Gdn.HCl, dissociation into subunits begins at a lower concentration of denaturant than the major changes in conformation.     

STRUCTURING MOVEMENT EXPERIENCES FOR PRE-SCHOOL CHILDREN

Summary It is proposed that a conceptual framework should be used as a basis for the provision of movement experiences for young children. Developmental levels and classifications of movement are considered in order to structure environments that provide suitable opportunities for self initiated movement for pre-school children.