全文获取类型
收费全文 | 1047篇 |
免费 | 47篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 29篇 |
妇产科学 | 14篇 |
基础医学 | 148篇 |
口腔科学 | 12篇 |
临床医学 | 76篇 |
内科学 | 284篇 |
皮肤病学 | 45篇 |
神经病学 | 91篇 |
特种医学 | 40篇 |
外科学 | 97篇 |
综合类 | 3篇 |
预防医学 | 41篇 |
眼科学 | 27篇 |
药学 | 83篇 |
中国医学 | 1篇 |
肿瘤学 | 94篇 |
出版年
2023年 | 15篇 |
2022年 | 18篇 |
2021年 | 31篇 |
2020年 | 13篇 |
2019年 | 18篇 |
2018年 | 22篇 |
2017年 | 12篇 |
2016年 | 24篇 |
2015年 | 23篇 |
2014年 | 32篇 |
2013年 | 46篇 |
2012年 | 84篇 |
2011年 | 87篇 |
2010年 | 43篇 |
2009年 | 54篇 |
2008年 | 70篇 |
2007年 | 63篇 |
2006年 | 50篇 |
2005年 | 69篇 |
2004年 | 50篇 |
2003年 | 43篇 |
2002年 | 46篇 |
2001年 | 4篇 |
2000年 | 18篇 |
1999年 | 15篇 |
1998年 | 13篇 |
1997年 | 13篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 13篇 |
1991年 | 13篇 |
1990年 | 9篇 |
1989年 | 9篇 |
1988年 | 12篇 |
1986年 | 6篇 |
1985年 | 3篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1976年 | 2篇 |
1974年 | 5篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1971年 | 2篇 |
1969年 | 2篇 |
1968年 | 1篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1965年 | 2篇 |
排序方式: 共有1100条查询结果,搜索用时 515 毫秒
91.
Genetic changes and histopathological types in colorectal tumors from patients with familial adenomatous polyposis 总被引:11,自引:0,他引:11
M Miyaki M Seki M Okamoto A Yamanaka Y Maeda K Tanaka R Kikuchi T Iwama T Ikeuchi A Tonomura 《Cancer research》1990,50(22):7166-7173
Loss of heterozygosity (LOH) and K-ras mutation were analyzed in 111 colorectal polyps and 26 invasive carcinomas from 40 patients with familial adenomatous polyposis of distinct histopathological types. LOH, being less than 2% in moderate adenomas, was detected on chromosome 5q (20%) in severe adenomas, on 5q (26%) and 17p (38%) in intramucosal carcinomas, and on 5q (52%), 17p (56%), 18 (46%), and 22q (33%) in invasive carcinomas. LOH on chromosome 5q occurred most frequently in the region close to the APC gene both in adenomas and carcinomas, and a loss of the normal allele of the APC gene was demonstrated in 3 cases. K-ras mutation markedly increased in the step of development from moderate (11%) to severe (36%) adenomas. These results suggest the following mechanisms for the development of colon tumors in patients with familial adenomatous polyposis: (a) the heterozygous mutant/wild-type condition at the APC gene causes formation of mild or moderate adenoma; (b) the loss of the normal allele in the APC gene leads to a change from moderate to severe adenoma; (c) LOH on chromosome 17p contributes to the conversion of adenoma to intramucosal carcinoma; (d) LOH on other chromosomes, such as 18 and 22q, are involved in the progression of intramucosal carcinoma to invasive carcinoma; and (e) K-ras mutation may also affect the development of moderate to severe adenoma. 相似文献
92.
93.
Lanthiopeptin, a new peptide antibiotic. Production, isolation and properties of lanthiopeptin 总被引:6,自引:0,他引:6
N Naruse O Tenmyo K Tomita M Konishi T Miyaki H Kawaguchi K Fukase T Wakamiya T Shiba 《The Journal of antibiotics》1989,42(6):837-845
A strain of Streptoverticillium cinnamoneum produced a peptide antibiotic named lanthiopeptin, which contained four unusual amino acids, erythro-beta-hydroxyaspartic acid, mesolanthionine, threo-beta-methyllanthionine and lysinoalanine. Lanthiopeptin showed antiviral activity against herpes simplex virus type 1 KOS strain infection in Vero cells by cytopathic effect reduction assay. The structure of lanthiopeptin is similar to that of ancovenin. 相似文献
94.
Miyaki M 《Nihon rinsho. Japanese journal of clinical medicine》2000,58(6):1225-1230
Germline mutations in one of two alleles of c-ret, c-met and c-kit protooncogenes have been revealed to be the causes of three autosomal dominant hereditary tumors; multiple endocrine neoplasia type 2(MEN2), hereditary papillary renal cell carcinoma (HPRCC), and familial gastrointestinal stromal tumor(FGIST), respectively. Patients with MEN2A have missense mutations at extracellular cysteine rich domain of c-ret, those with MEN2B have missense mutations at tyrosine kinase domain of c-ret, those with HPRCC have missense mutations at tyrosine kinase domain of c-met, and those with FGIST have in-frame deletion mutations at juxtamembrane domain of c-kit. All of these mutations are assumed to cause constitutive activation of protooncogenes without binding to ligands, resulting in tumor formation. 相似文献
95.
Hiroshi Kumagai Asako Zempo-Miyaki Toru Yoshikawa Takehiko Tsujimoto Kiyoji Tanaka Seiji Maeda 《Journal of Clinical Biochemistry and Nutrition》2016,58(1):84-89
Obesity has reached epidemic proportions worldwide. Obesity results in reduced serum testosterone levels, which causes many disorders in men. Lifestyle modifications (increased physical activity and calorie restriction) can increase serum testosterone levels. However, it is unknown whether increased physical activity or calorie restriction during lifestyle modifications has a greater effects on serum testosterone levels. Forty-one overweight and obese men completed a 12-week lifestyle modification program (aerobic exercise training and calorie restriction). We measured serum testosterone levels, the number of steps, and the total energy intake. We divided participants into two groups based on the median change in the number of steps (high or low physical activities) or that in calorie restriction (high or low calorie restrictions). After the program, serum testosterone levels were significantly increased. Serum testosterone levels in the high physical activity group were significantly higher than those in the low activity group. This effect was not observed between the groups based on calorie restriction levels. We found a significant positive correlation between the changes in serum testosterone levels and the number of steps. Our results suggested that an increase in physical activity greatly affected the increased serum testosterone levels in overweight and obese men during lifestyle modification. 相似文献
96.
Reiji Higashiyama Shigeru Miyaki Satoshi Yamashita Teruhito Yoshitaka Görel Lindman Yoshiaki Ito Takahisa Sasho Kazuhisa Takahashi Martin Lotz Hiroshi Asahara 《Modern rheumatology / the Japan Rheumatism Association》2010,20(1):11-17
Recent studies suggest that histone deacetylase (HDAC) inhibitors may therapeutically prevent cartilage degradation in osteoarthritis
(OA). Matrix metalloproteinase-13 (MMP-13) plays an important role in the pathogenesis of this disease and in the present
study we investigated the correlation between HDACs and MMP-13. Comparing the expression of different HDACs in cartilage from
OA patients and healthy donors, HDAC7 showed a significant elevation in cartilage from OA patients. High level of HDAC7 expression
in OA cartilage was also confirmed by immunohistochemistry. Knockdown of HDAC7 by small interference RNA (siRNA) in SW1353
human chondrosarcoma cells strongly suppressed interleukin (IL)-1-dependent and independent induction of MMP-13 gene expression.
In conclusion, elevated HDAC7 expression in human OA may contribute to cartilage degradation via promoting MMP-13 gene expression,
suggesting the critical role of MMP-13 in OA pathogenesis. 相似文献
97.
Katsutoshi Sato Kazumasa Orihashi Tatsuya Kurosaki †Asako Tokumine †Shintaro Fukunaga †Shinji Ninomiya Taijiro Sueda 《Artificial organs》2009,33(4):352-359
Abstract: In order to develop a diaphragm-type ventricular assist device (VAD), we studied the flow field change following structural modifications. We devised a center flow-type pump by putting a small projection on the center of the housing and/or diaphragm to provide a center in the flow field, and examined the following four types of VADs: N type without a projection, D type with a projection on the diaphragm, H type with a projection on the housing, and DH type with projections on both the diaphragm and housing. Computational fluid dynamics (CFD) was used for flow simulation. Particle image velocimetry (PIV) was also used to verify the reliability of the CFD method and to determine how the flow field changes in the presence of a projection. The results of the PIV and CFD analyses were comparable. The placement of a projection on the housing was most effective in rectifying the flow field. 相似文献
98.
99.
Daisuke Usuda Toshihide Izumida Nao Terada Ryusho Sangen Toshihiro Higashikawa Sayumi Sekiguchi Risa Tanaka Makoto Suzuki Yuta Hotchi Shintaro Shimozawa Shungo Tokunaga Ippei Osugi Risa Katou Sakurako Ito Suguru Asako Yoshie Takagi Kentaro Mishima Akihiko Kondo Keiko Mizuno Hiroki Takami Takayuki Komatsu Jiro Oba Tomohisa Nomura Manabu Sugita Yuji Kasamaki 《World Journal of Clinical Cases》2021,9(23):6886-6899
100.
Atsuhiro Ichihara Mariyo Sakoda Asako Kurauchi-Mito Tatsuya Narita Kenichiro Kinouchi Hiroshi Itoh 《Journal of pharmacological sciences》2009,109(1):20-23
Discovery of the (pro)renin receptor uncovered a novel function of renin/prorenin as the receptor ligands in addition to the enzyme and its precursor. The bindings of renin and prorenin to the (pro)renin receptor trigger two major pathways: the angiotensin II–dependent pathway as a result of the enzymatic activation of renin/prorenin and the angiotensin II– independent intracellular pathway involving hypertrophic, hyperplastic, and profibrotic signals. A specific blocker of the receptor was discovered through identification of the amino acid sequence of prorenin prosegment that binds to the receptor and leads to non-proteolytic conversion of prorenin to its active form. A peptide containing this sequence was found to block the binding of prorenin to its receptor. Its infusion in animal models of diabetes and low-renin hypertension significantly inhibited the development and progression of nephropathy, but (pro)renin receptor blockade had no benefit in the clipped kidney of 2K1C rats or rat models of high-renin hypertension. Since renin is still active without a (pro)renin receptor, (pro)renin-receptor blockade elicits a maximum benefit under low-renin conditions. Thus, (pro)renin-receptor blockade can be a useful therapy for chronic kidney disease with low renin levels in the plasma. 相似文献