全文获取类型
收费全文 | 276篇 |
免费 | 8篇 |
专业分类
儿科学 | 1篇 |
妇产科学 | 9篇 |
基础医学 | 15篇 |
口腔科学 | 3篇 |
临床医学 | 12篇 |
内科学 | 72篇 |
皮肤病学 | 2篇 |
神经病学 | 12篇 |
特种医学 | 7篇 |
外科学 | 94篇 |
预防医学 | 3篇 |
眼科学 | 7篇 |
药学 | 10篇 |
肿瘤学 | 37篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 10篇 |
2020年 | 2篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 6篇 |
2015年 | 7篇 |
2014年 | 6篇 |
2013年 | 13篇 |
2012年 | 31篇 |
2011年 | 21篇 |
2010年 | 10篇 |
2009年 | 10篇 |
2008年 | 21篇 |
2007年 | 18篇 |
2006年 | 25篇 |
2005年 | 21篇 |
2004年 | 7篇 |
2003年 | 18篇 |
2002年 | 16篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1977年 | 4篇 |
1975年 | 1篇 |
1974年 | 6篇 |
1973年 | 2篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1968年 | 3篇 |
排序方式: 共有284条查询结果,搜索用时 15 毫秒
101.
Aristotelis Perrakis Klaus Weber Susanne Merkel Klaus Matzel Abbas Agaimy Carol Gebbert Werner Hohenberger 《International journal of colorectal disease》2014,29(10):1223-1229
Purpose
Complete mesocolic excision (CME) is nowadays state of the art in the treatment of colon cancer. In cases of carcinoma of transverse colon and of both flexures an extramesocolic lymph node metastasis can be found in the infrapancreatic lymph node region (ILR) and across the gastroepiploic arcade (GLR). These direct metastatic routes were not previously systematically considered. In order to validate our hypothesis of these direct metastatic pathways and to obtain evidence of our approach of including dissection of these areas as part of CME, we initiated a prospective study evaluating these lymph node regions during surgery.Methods
Forty-five consecutive patients with primary tumour manifestation in transverse colon and both flexures between May 2010 and January 2013 were prospectively analyzed. Patients were followed up for at least 6 months. Mode of surgery, histopathology, morbidity and mortality were evaluated.Results
Twenty-six patients had a carcinoma of transverse colon, 16 patients one of hepatic flexure and four patients one of splenic flexure. The median lymph node yield was 40. Occurrence of lymph node metastasis in ILR was registered in five patients and in GLR in four patients. The mean lymph node ratio was 0.085. Postoperative complications occurred in nine patients, and postoperative mortality was 2 %.Conclusions
We were able to demonstrate this novel metastatic route of carcinomas of the transverse colon and of both flexures in ILR and GLR. These could be considered as regional lymph node regions and have to be included into surgery for cancer of the transverse colon including both flexures. 相似文献102.
103.
104.
Rachael J. Almond Brian F. Flanagan Aristotelis Antonopoulos Stuart M. Haslam Anne Dell Ian Kimber Rebecca J. Dearman 《European journal of immunology》2013,43(1):170-181
Human native milk lactoferrin (LF) and recombinant forms of lactoferrin (rLF) are available with identical aa sequences, but different glycosylation patterns. Native lactoferrin (NLF) possesses the intrinsic ability to stimulate vigorous IgG and IgE antibody responses in BALB/c mice, whereas recombinant forms (Aspergillus or rice) are 40‐fold less immunogenic and 200‐fold less allergenic. Such differences are independent of endotoxin or iron content and the glycans do not contribute to epitope formation. A complex glycoprofile is observed for NLF, including sialic acid, fucose, mannose, and Lewis (Le)x structures, whereas both rLF species display a simpler glycoprofile rich in mannose. Although Lex type sugars play a Th2‐type adjuvant role, endogenous expression of Lex on NLF did not completely account for the more vigorous IgE responses it provoked. Furthermore, coadminstration of rLF downregulated IgE and upregulated IgG2a antibody responses provoked by NLF, but was without effect on responses to unrelated peanut and chicken egg allergens. These results suggest glycans on rLF impact the induction phase to selectively inhibit IgE responses and that differential glycosylation patterns may impact on antigen uptake, processing and/or presentation, and the balance between Th1 and Th2 responses. 相似文献
105.
Chi Y. Lo Aristotelis Antonopoulos Anne Dell Stuart M. Haslam Techung Lee Sriram Neelamegham 《Biomaterials》2013
Mesenchymal stem/stromal cells (MSCs) are an important candidate for cell-based therapy since they can be easily isolated and expanded, secrete beneficial paracrine factors, and differentiate into multiple lineages. Since the endothelium at sites of injury and inflammation often express adhesion molecules belonging to the selectin family, methods to endow MSCs with selectin-ligands can enhance the efficacy of cell delivery and tissue engraftment. Here, we describe a construct 19Fc[FUT7+], where the first 19 amino acids of the pan-selectin ligand PSGL-1 (P-selectin glycoprotein ligand-1) was fused to a human IgG1. When expressed in HEK293T cells over-expressing the α(1,3)fucosyltransferase FUT7, 19Fc[FUT7+] is decorated by a core-2 sialyl Lewis-X sialofucosylated O-glycan. The non-covalent coupling of this protein onto MSC surface using palmitated protein G (PPG) enhanced cell binding to E- and P-selectin under hydrodynamic shear, without altering MSC multipotency. MSCs functionalized with 19Fc[FUT7+] were captured/tethered onto stimulated endothelial cell monolayers at wall shear stresses up to 4 dyn/cm2. Once captured, the cells rolled robustly up to the highest shear stress tested, 10 dyn/cm2. Unlike previous work where MSCs could only be captured onto selectin-bearing substrates at low or no-flow conditions, the current work presents a ‘glycan engineering’ strategy to enable leukocyte-like capture and rolling. 相似文献
106.
Divaris K Ntounis A Marinis A Polyzois GL Polychronopoulou A 《The International journal of prosthodontics》2012,25(2):145-147
This study investigated factors associated with perceptions of complete dentures (CDs) among patients presenting at a university dental clinic. A retrospective chart review of all new edentulous patients presenting at the Athens University School of Dentistry Removable Prosthodontics Clinic between 1993 and 2002 (n = 2,526) was completed. More than half of CD-wearing patients were dissatisfied with their dentures at the initial visit. Dissatisfaction was correlated with current use of denture adhesives and less time spent edentulous and was more prevalent among females. Factors affecting patients? adaptation to and satisfaction with CDs should be considered at CD fabrication and follow-up. 相似文献
107.
Aristotelis Kotronoulas Hulda S. Jnasdttir Rsa S. Sigurardttir Skarphinn Halldrsson Gumundur G. Haraldsson
ttar Rolfsson 《Journal of tissue engineering and regenerative medicine》2020,14(3):441-451
Acellular fish skin (ACS) has emerged as a dermal substitute used to promote wound healing with decreased scar formation and pain relief that may be due to polyunsaturated fatty acid (PUFA) content. However, the PUFA content of ACS is still unknown. The aim of this study was to compare the total fatty acids and lipid profiles of ACS to two bovine‐based grafts and standard of care human cadaver skin (HCS). Furthermore, there was also the goal to assess the capability of ACS lipid content to enhance wound healing. The fatty acid analysis was performed with GC–FID, and an LC–MS untargeted method was developed in order to the analyse the lipid profiles of the grafts was. The enhancement of wound healing by the ACS extract was investigated in vitro on HaCat cells. Our results showed that ACS had the highest content of PUFA (27.0 ± 1.43% of their total fatty acids), followed by HCS (20.6 ± 3.9%). The two grafts of bovine origin presented insignificant PUFA amounts. The majority of the PUFAs found in ACS were omega‐3, and in HCS, they were omega‐6. The untargeted lipidomics analysis demonstrated that ACS grafts were characterized by phosphatidylcholine containing either 20:5 or 22:6 omega‐3 PUFA. The ACS lipid extract increased the HaCat cells migration and enhanced wound closure 4 hr earlier versus control. Our study demonstrated that ACS has a lipid profile that is distinct from other wound healing grafts, that PUFAs are maintained in ACS post‐processing as phosphatidylcholine, and that ACS lipid content influences wound healing properties. 相似文献
108.
Johannes Hartl Lee Serpas Yueyang Wang Ali Rashidfarrokhi Oriana A. Perez Benjamin Sally Vanja Sisirak Chetna Soni Alireza Khodadadi-Jamayran Aristotelis Tsirigos Ivan Caiello Claudia Bracaglia Stefano Volpi Gian Marco Ghiggeri Asiya Seema Chida Ignacio Sanz Mimi Y. Kim H. Michael Belmont Gregg J. Silverman Robert M. Clancy Peter M. Izmirly Jill P. Buyon Boris Reizis 《The Journal of experimental medicine》2021,218(5)
Antibodies to double-stranded DNA (dsDNA) are prevalent in systemic lupus erythematosus (SLE), particularly in patients with lupus nephritis, yet the nature and regulation of antigenic cell-free DNA (cfDNA) are poorly understood. Null mutations in the secreted DNase DNASE1L3 cause human monogenic SLE with anti-dsDNA autoreactivity. We report that >50% of sporadic SLE patients with nephritis manifested reduced DNASE1L3 activity in circulation, which was associated with neutralizing autoantibodies to DNASE1L3. These patients had normal total plasma cfDNA levels but showed accumulation of cfDNA in circulating microparticles. Microparticle-associated cfDNA contained a higher fraction of longer polynucleosomal cfDNA fragments, which bound autoantibodies with higher affinity than mononucleosomal fragments. Autoantibodies to DNASE1L3-sensitive antigens on microparticles were prevalent in SLE nephritis patients and correlated with the accumulation of cfDNA in microparticles and with disease severity. DNASE1L3-sensitive antigens included DNA-associated proteins such as HMGB1. Our results reveal autoantibody-mediated impairment of DNASE1L3 activity as a common nongenetic mechanism facilitating anti-dsDNA autoreactivity in patients with severe sporadic SLE. 相似文献
109.
110.
Michael Luna MD Aristotelis Papayannis MD Elizabeth M. Holper MD MPH FSCAI Subhash Banerjee MD FSCAI Emmanouil S. Brilakis MD PhD FSCAI 《Catheterization and cardiovascular interventions》2012,80(3):437-446
The GuideLiner catheter (Vascular Solutions, Minneapolis, MN) is a novel, rapid exchange catheter that allows deep vessel intubation. We describe 21 patients in whom the GuideLiner catheter [7 French (F) in 76% and 6F in 24%] was used to facilitate equipment delivery (n = 14) or vessel engagement (n = 7). Pressure dampening after GuideLiner catheter insertion was observed in 12 patients (57%). The procedure was successfully completed in 19 patients (90%) and one patient developed acute vessel closure, likely due to dissection. The GuideLiner catheter can facilitate complex coronary interventions but should be used with caution to minimize the risk for vessel injury. © 2011 Wiley Periodicals, Inc. 相似文献