全文获取类型
收费全文 | 31473篇 |
免费 | 1507篇 |
国内免费 | 278篇 |
专业分类
耳鼻咽喉 | 361篇 |
儿科学 | 784篇 |
妇产科学 | 699篇 |
基础医学 | 3736篇 |
口腔科学 | 1794篇 |
临床医学 | 2442篇 |
内科学 | 6860篇 |
皮肤病学 | 609篇 |
神经病学 | 2112篇 |
特种医学 | 716篇 |
外科学 | 3763篇 |
综合类 | 1168篇 |
现状与发展 | 2篇 |
一般理论 | 34篇 |
预防医学 | 3696篇 |
眼科学 | 424篇 |
药学 | 2215篇 |
2篇 | |
中国医学 | 505篇 |
肿瘤学 | 1336篇 |
出版年
2024年 | 31篇 |
2023年 | 279篇 |
2022年 | 360篇 |
2021年 | 845篇 |
2020年 | 531篇 |
2019年 | 746篇 |
2018年 | 1170篇 |
2017年 | 750篇 |
2016年 | 760篇 |
2015年 | 971篇 |
2014年 | 1111篇 |
2013年 | 1435篇 |
2012年 | 2539篇 |
2011年 | 2764篇 |
2010年 | 1495篇 |
2009年 | 1143篇 |
2008年 | 2146篇 |
2007年 | 2214篇 |
2006年 | 2005篇 |
2005年 | 1866篇 |
2004年 | 1610篇 |
2003年 | 1435篇 |
2002年 | 1317篇 |
2001年 | 546篇 |
2000年 | 601篇 |
1999年 | 411篇 |
1998年 | 129篇 |
1997年 | 74篇 |
1996年 | 68篇 |
1995年 | 60篇 |
1994年 | 54篇 |
1993年 | 56篇 |
1992年 | 235篇 |
1991年 | 164篇 |
1990年 | 146篇 |
1989年 | 124篇 |
1988年 | 112篇 |
1987年 | 105篇 |
1986年 | 90篇 |
1985年 | 79篇 |
1984年 | 67篇 |
1983年 | 60篇 |
1979年 | 31篇 |
1975年 | 41篇 |
1974年 | 52篇 |
1973年 | 30篇 |
1971年 | 36篇 |
1970年 | 37篇 |
1969年 | 32篇 |
1968年 | 38篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
82.
83.
目的研究1例17α-羟化酶/17,20-裂解酶部分性联合缺陷症患者CYP17A1基因突变特点,并结合患者的临床表现与基因突变类型初步探讨P450C17酶蛋白的结构与功能的关系。方法收集1例17α-羟化酶/17,20-裂解酶部分性联合缺陷症患者的临床资料及其亲属血标本,提取基因组DNA,设计7对引物扩增CYP17A1基因的8个外显子及外显子与内含子的连接区域,琼脂糖凝胶电泳鉴定PCR产物,产物胶回收后直接做为DNA双链模板测序。DNA双链模板不一致的PCR产物经克隆后测序。测序结果在核苷酸序列数据库进行比较分析。结果患者CYP17A1基因突变检测结果为5994-5995delAT/7541C>T复合杂合子。这两种突变均未见报道。推测5994-5995delAT导致I259H,274X,突变形成的截短蛋白质缺少血红素结合区域,因此是没有功能的;而通过人类P450C17酶计算机模型分析显示7541C>T导致的A398V远离酶的活性中心,推测突变可能使酶的活性减弱,而不是完全地丧失。患者临床表现为有自发不规则月经及轻度高血压、低血钾,结合激素测定结果提示肾上腺和性腺保留部分功能。因而患者的基因型与其临床表型是一致的。结论应进行突变P450C17酶的功能学研究来进一步明确结构改变对功能的影响。 相似文献
84.
Elisa Moya-Sáez Rafael Navarro-González Santiago Cepeda Ángel Pérez-Núñez Rodrigo de Luis-García Santiago Aja-Fernández Carlos Alberola-López 《NMR in biomedicine》2022,35(9):e4754
Glioblastoma is an aggressive and fast-growing brain tumor with poor prognosis. Predicting the expected survival of patients with glioblastoma is a key task for efficient treatment and surgery planning. Survival predictions could be enhanced by means of a radiomic system. However, these systems demand high numbers of multicontrast images, the acquisitions of which are time consuming, giving rise to patient discomfort and low healthcare system efficiency. Synthetic MRI could favor deployment of radiomic systems in the clinic by allowing practitioners not only to reduce acquisition time, but also to retrospectively complete databases or to replace artifacted images. In this work we analyze the replacement of an actually acquired MR weighted image by a synthesized version to predict survival of glioblastoma patients with a radiomic system. Each synthesized version was realistically generated from two acquired images with a deep learning synthetic MRI approach based on a convolutional neural network. Specifically, two weighted images were considered for the replacement one at a time, a T2w and a FLAIR, which were synthesized from the pairs T1w and FLAIR, and T1w and T2w, respectively. Furthermore, a radiomic system for survival prediction, which can classify patients into two groups (survival >480 days and 480 days), was built. Results show that the radiomic system fed with the synthesized image achieves similar performance compared with using the acquired one, and better performance than a model that does not include this image. Hence, our results confirm that synthetic MRI does add to glioblastoma survival prediction within a radiomics-based approach. 相似文献
85.
Dias EP Rocha ML Silva JúniorA Spyrides KS Ferreira SM Polignano GA Feijó EC Da Fonseca EC 《American journal of clinical pathology》2000,114(3):395-401
Accurate diagnosis of oral hairy leukoplakia (OHL) is important because it may be an early indicator of undiagnosed HIV infection; moreover, it may be a prognostic indicator. Our purpose was to investigate the histopathologic features of subclinical OHL and to evaluate and support the rationale of detecting subclinical OHL with cytopathology. The Epstein-Barr virus (EBV) was detected by immunohistochemistry and in situ hybridization in 4 cases of macroscopically normal lateral borders of tongue mucosa from 8 AIDS necropsies and in none of 8 controls. The histopathologic features were specific when based on nuclear changes: Cowdry type A inclusion, ground glass, and nuclear beading. Smears were obtained from 50 patients with AIDS, without OHL, from the scraping of lateral borders of the tongue. Numerous clusters of the cells were associated with Candida organisms (30% of cases). Nuclear changes were observed in 12 patients (24%) on both sides of the tongue. We describe the histopathologic features of subclinical OHL, and our observations suggest that cytopathology can detect OHL in the subclinical phase. 相似文献
86.
Velayos A Fuentes-Vicente M Aguilar-Elena R Eslava AP Iturriaga EA 《Current genetics》2004,45(6):371-377
Two Mucor circinelloides
structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes.Abbreviations FPP
farnesyl diphosphate (or pyrophosphate)
- GGPP
geranylgeranyl diphosphate
- PrenylPP
prenyl diphosphate
- DPP
decaprenyl diphosphate
- HPP
hexaprenyl diphosphate
- SPP
solanesyl diphosphate 相似文献
87.
In this paper, the specific absorption rate (SAR) in scaled human head models is analysed to study possible differences between SAR in the heads of adults and children and for assessment of compliance with the international safety guidelines, while using a mobile phone. The finite-difference time-domain method (FDTD) has been used for calculating SAR values for models of both children and adults, at 900 and 1800 MHz. Maximum 1 g averaged SAR (SAR1 g) and maximum 10 g averaged SAR (SAR10 g) have been calculated in adults and scaled head models for comparison and assessment of compliance with ANSI/IEEE and European guidelines. Results show that peak SAR1 g and peak SAR10 g all trend downwards with decreasing head size but as head size decreases, the percentage of energy absorbed in the brain increases. So, higher SAR in children's brains can be expected depending on whether the thickness of their skulls and surrounding tissues actually depends on age. The SAR in eyes of different sizes, as a critical organ, has also been studied and very similar distributions for the full size and the scaled models have been obtained. Standard limits can only be exceeded in the unpractical situation where the antenna is located at a very short distance in front of the eye. 相似文献
88.
Concepción Núñez Diana Alecsandru Jezabel Varadé Isabel Polanco Carlos Maluenda Miguel Fernández-Arquero Emilio G de la Concha Elena Urcelay Alfonso Martínez 《BMC medical genetics》2006,7(1):32-5
Background
Celiac disease (CD) is a chronic disorder characterized by a pathological inflammatory response after exposure to gluten in genetically susceptible individuals. The HLA complex accounts for less than half of the genetic component of the disease, and additional genes must be implicated. Interleukin-10 (IL-10) is an important regulator of mucosal immunity, and several reports have described alterations of IL-10 levels in celiac patients. The IL-10 gene is located on chromosome 1, and its promoter carries several single nucleotide polymorphisms (SNPs) and microsatellites which have been associated to production levels. Our aim was to study the role of those polymorphisms in susceptibility to CD in our population. 相似文献89.
Zúñiga MC 《Virus research》2002,88(1-2):17-33
The poxviruses have evolved a diverse array of proteins which serve to subvert innate and adaptive host responses that abort or at least limit viral infections. Myxoma virus and its rabbit host are considered to represent an ideal poxvirus-host system in which to study the effects of these immunomodulatory proteins. Studies of laboratory rabbits (Oryctolagus cuniculus) infected with gene knockout variants of myxoma virus have provided compelling evidence that several myxoma virus gene products contribute to the pathogenic condition known as myxomatosis. However, myxomatosis, which is characterized by skin lesions, systemic immunosuppression, and a high mortality rate, does not occur in the virus' natural South American host, Sylvilogus brasiliensis. Moreover, in Australia where myxoma virus was willfully introduced to control populations of O. cuniculus, myxomatosis-resistant rabbits emerged within a year of myxoma virus introduction into the field. In this review I discuss the characterized immunomodulatory proteins of myxoma virus, their biochemical properties, their pathogenic effects in laboratory rabbits, the role of the host immune system in the susceptibility or resistance to myxomatosis, and the evidence that immunomodulatory genes may have been attenuated during the co-adaptation of myxoma virus and O. cuniculus in Australia. 相似文献
90.
Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used 总被引:5,自引:0,他引:5 下载免费PDF全文
Velikovsky CA Goldbaum FA Cassataro J Estein S Bowden RA Bruno L Fossati CA Giambartolomei GH 《Infection and immunity》2003,71(10):5750-5755
The immunogenicity and protective efficacy of recombinant lumazine synthase from Brucella spp. (rBLS) administered with different adjuvants was evaluated in mice. Mice were immunized with rBLS in the absence or the presence of aluminum hydroxide gel (BLS-Al), monophosphoryl lipid A (BLS-MPA), or incomplete Freund's adjuvant (BLS-IFA). rBLS per se induced a vigorous immunoglobulin G (IgG) response, with high titers of IgG1 as well as IgG2. All the adjuvants increased this response; the BLS-IFA formulation was the most effective at inducing BLS-specific IgG antibodies. In addition, after in vitro stimulation with rBLS, spleen cells from BLS-IFA-, BLS-Al-, or BLS-MPA-immunized mice proliferated and produced interleukin-2 (IL-2), gamma interferon (IFN-gamma), IL-10, and IL-4, suggesting the induction of a mixed Th1-Th2 response. Immunization with rBLS protected mice against challenge with B. abortus 544. The levels of protection in the spleen were similar for all adjuvants, but only BLS-Al and BLS-IFA were effective in the liver. Our results indicate that BLS might be a useful candidate for the development of subunit vaccines against brucellosis, since it elicits antigen-specific cellular responses, with production of IFN-gamma and protection, independently of the adjuvant formulation used. 相似文献