全文获取类型
收费全文 | 187篇 |
免费 | 4篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 2篇 |
妇产科学 | 1篇 |
基础医学 | 14篇 |
口腔科学 | 3篇 |
临床医学 | 13篇 |
内科学 | 47篇 |
皮肤病学 | 8篇 |
神经病学 | 19篇 |
特种医学 | 4篇 |
外科学 | 41篇 |
综合类 | 12篇 |
预防医学 | 22篇 |
眼科学 | 2篇 |
药学 | 4篇 |
出版年
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 2篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 5篇 |
2014年 | 5篇 |
2013年 | 15篇 |
2012年 | 20篇 |
2011年 | 16篇 |
2010年 | 26篇 |
2009年 | 4篇 |
2008年 | 13篇 |
2007年 | 8篇 |
2006年 | 15篇 |
2005年 | 11篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1990年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有192条查询结果,搜索用时 31 毫秒
31.
32.
Anupa Dey Snezana Kusljic Richard J Lang Betty Exintaris 《British journal of pharmacology》2010,161(8):1692-1707
BACKGROUND AND PURPOSE
To investigate the role of connexin 43 in the maintenance of spontaneous activity in prostate tissue from young and old guinea pigs.EXPERIMENTAL APPROACH
Conventional intracellular microelectrode and tension recording techniques, coupled with Western blot analysis and immunohistochemistry for connexin 43 (CX43) were used. The effects of three gap junction uncouplers, 18β glycyrrhetinic acid (10 µM, 40 µM), carbenoxolone (10 µM, 50 µM) and octanol (0.5 mM, 1 mM), were studied in cells displaying slow wave activity and on spontaneously contracting tissue from prostate glands of young (2–5 months) and old (9–16 months) guinea pigs.KEY RESULTS
18β Glycyrrhetinic acid (40 µM), carbenoxolone (50 µM) or octanol (0.5 mM) abolished slow wave activity in prostate tissue from young and old guinea pigs and depolarized membrane potential by approximately 5 mV. These treatments also abolished all contractions in both sets of prostate tissue. These effects were reversed upon washout. Western blot analysis and CX43 immunohistochemistry showed that there was no age-related difference in the expression and distribution of CX43 in prostate tissues.CONCLUSION AND IMPLICATIONS
When gap junctional communication via CX43 was disrupted, spontaneous activity was abolished at a cellular and whole tissue level; CX43 is therefore essential for the maintenance of spontaneous slow wave activity and subsequent contractile activity in the guinea pig prostate gland. 相似文献33.
Fifty (50) cases of Leishmaniasis were included in a prospective study after making the diagnosis based on the demonstration of L D Bodies on bone marrow aspirate. Hemoglobin (Hb) and total leucocyte count (TLC) were less than 10 gm/dl and 4000 per/ml respectively in 22 out of 50 cases. Among 50 cases, 30 showed macrocytic red cell and hypersegmented neutrophils on peripheral blood smear as features of megaloblastic anemia. Out of 30 cases of leishmaniasis with megaloblastic anemia, 10 cases showed decreased reticulocyte count. Bone marrow aspiration cytology showed megaloblastic maturation in erythroid series and giant metamyelocytes in myeloid series in all 30 cases of leishmaniasis with megaloblastic changes. Bone marrow iron was also increased in these cases. However megakaryocyte was normal in all. Among 30 cases of leishmaniasis with megaloblastic changes, 15 cases were treated with sodium antimony gluconate (SAG) only and other 15 with SAG, folic acid and Vit. B12. Rest 20 cases of leishmaniasis without megaloblastic changes were also treated with SAG only. After treatment with SAG alone as well as with SAG, folic acid and vit. B12, hemoglobin and TLC improved in all 50 cases. Of 15 cases of leishmaniasis with megaloblastic changes, who were treated with SAG, folic acid and vit. B12, the reticulocyte count reached at peak on the 7th day and normalized at the end of 4th week. Red blood cells (RBC) also became normocytic normochromic and neutrophils became normal on peripheral blood smear. But no change was observed in reticulocyte count, RBC and white blood cell (WBC) morphology on peripheral blood smear in cases of leishmaniasis without megaloblastic changes and other 15 cases of leishmaniasis with megaloblastic changes, who received SAG only. 相似文献
34.
35.
Microbial risk assessment for recreational use of the Chicago area waterway system 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《Journal of water and health》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rijal G Tolson JK Petropoulou C Granato TC Glymph A Gerba C Deflaun MF O'Connor C Kollias L Lanyon R 《Journal of water and health》2011,9(1):169-186
A microbial risk assessment was conducted to estimate the human health risks from incidental contact recreational activities such as canoeing, boating and fishing in the Chicago Area Waterway System (CAWS) receiving secondary treated, but non-disinfected, effluent from three municipal water reclamation plants. Actual concentrations of the pathogens (pathogenic E. coli [estimated], Giardia, Cryptosporidium, adenovirus, norovirus, enteric virus) detected from the waterway field data collection at locations upstream and downstream of the effluent outfall during dry and wet weather conditions within the recreation season were included in the risk assessment. The results under the current treatment scheme with no disinfection indicated that the total expected gastrointestinal illness (GI) rate per 1000 incidental contact recreational exposure events during combined weather (dry and wet) conditions ranged from 0.10 to 2.78 in the CAWS, which is below the eight illnesses per 1000 swimmers considered tolerable by the United States Environmental Protection Agency. Wet weather conditions contribute to elevated pathogen load to the CAWS; therefore this study determined that disinfecting the effluents of three major WRPs that discharge to the CAWS would result in an extremely small reduction in the aggregate recreation season risk to incidental contact recreators. 相似文献
36.
Rajan Chamlagain Sangam Shah Bijay Rijal Roman Dhital Bipin Kandel Basanta Sharma Paudel 《Clinical Case Reports》2021,9(8)
We report this case to focus on the importance of early diagnosis, challenges to reach the diagnosis in a low‐resource setting, and management that may result in a better prognosis of the patients. 相似文献
37.
38.
39.
Laxman Rijal Hemant Manandhar Pravin Nepal Md Tahir Ansari C. S. Yadhav 《European journal of orthopaedic surgery & traumatology : orthopedie traumatologie》2010,20(6):505-507
Intramedullary interlocking nail has become standard mode of treatment for fracture of long bones of leg. Where instrumentations,
like flexible reamers, are restraint, it can be sufficed with serial reaming with straight reamer. However, straight reamer
makes the surgery difficult at times, because it has a tendency to break. We encountered a similar situation and would like
to share how we retrieved the broken piece of straight reamer from the medullary canal. 相似文献
40.
Burniston I Roy L Picado A Das M Rijal S Rogers M Coosemans M Boelaert M Davies C Cameron M 《Journal of medical entomology》2010,47(5):902-906
Anthroponotic visceral leishmaniasis, transmitted by Phlebotomus argentipes Annandale & Brunetti (Diptera: Psychodidae) sand flies, is regarded as a major problem of public health importance in the Indian subcontinent. Understanding the feeding behavior of the vector can be used to investigate changes in human-vector contact during intervention programs. An enzyme-linked immunosorbent assay (ELISA) was modified to make it suitable to identify the origin of P. argentipes and Phlebotomus papatasi Scopoli (Diptera: Psychodidae) blood meals. The sensitivity and specificity of the precipitin ring test and ELISA were compared, as well as the stability of the tests across different stages of blood meal digestion. The ELISA was more sensitive and specific than the precipitin test for identifying the sources of blood meals. When using the ELISA method with a plate reader, it was possible to obtain 100% sensitivity and specificity. When comparing the techniques across digestion stages, it was found that there was a drop in sensitivity, 48 and 72 h postblood meal for precipitin and visually read ELISA, respectively. However, the sensitivity of the ELISA using a plate reader was not altered by the digestion time. The feeding habits of P. argentipes and P. papatasi from the Terai region of Nepal, determined by the ELISA developed, showed P. papatasi to be highly anthropophilic, and P. argentipes appeared to feed both on humans and animals, in particular bovines. 相似文献