结核分枝杆菌MPT64抗原对巨噬细胞凋亡的抑制作用

目的探讨结核分枝杆菌MPT64抗原对RAW264.7巨噬细胞的影响及相关机制。方法将MPT64基因在大肠杆菌中表达、纯化,获得的纯化蛋白用于后续实验。将用佛波醇酯(PMA)分化的RAW264.7巨噬细胞分为对照组、卡介菌纯蛋白衍生物(BCGPPD)诱导组、BCG-PPD+MPT64共同作用组,分别给予相应的处理。孵育16 h后,采用流式细胞术检测细胞凋亡情况,用ELISA试剂盒检测培养细胞上清的细胞因子TNF-α及IL-10水平。结果 BCG-PPD可以诱导RAW264.7巨噬细胞凋亡,BCG-PPD+MPT64组的细胞凋亡水平低于BCG-PPD组(P<0.05)。细胞因子上清检测结果表明,与对照组相比,BCG-PPD作用组的TNF-α水平升高(P<0.01),而IL-10水平无明显变化;与BCG-PPD组相比,BCG-PPD+MPT64共同孵育组的IL-10水平升高(P<0.01),而TNF-α水平无明显变化。结论 MPT64抗原可能是一种毒力因子,能够抑制BCG-PPD诱导的巨噬细胞凋亡,该作用可能是通过提高IL-10水平来实现的。     

Development and Evaluation of a MAb-Based ELISA for Detection of Chlamydophila pneumoniae Infection with Variable Domain 2 and 3 of the Major Outer Membrane protein

ObjectiveThis paper aims to develop a monoclonal antibodies (MAbs)- based ELISA for detecting Chlamydophila pneumoniae (C. pneumoniae) antigens in humans with the variable domains (VD) 2 and 3 of the major outer membrane protein (MOMP_VD2-VD3) and to assess its sensitivity and specificity by comparing with a widely used MAb that is able to recognize the elementary bodies of C. pneumoniae.MethodsMOMP_VD2-VD3 were overexpressed in Escherichia coli and purified by affinity chromatography. Mice were immunized with the recombinant antigen, and hybridomas secreting MAbs were screened. Three stable hybridomas clones were selected and named 5D6, 7G3, and 8C9. The MAbs-based ELISA was scrutinized for species-specific recognition with a number of human throat swab samples from Group I (156 patients with typical respiratory illness clinically confirmed before) and Group II (57 healthy donors).ResultsIn Group I, 55 positive cases were detected by anti-EB MAb-based ELISA, 51 cases were positive by MAbs 5D6-based ELISA, and 33 and 38 cases were positive by MAb 8C9 and 7G3-based ELISA respectively. Of the 57 samples from Group II “healthy donors”, 5 were positive and 52 were negative with both anti-EB and 5D6-based tests, while 2 and 3 positive cases were identified by the other two MAb-based ELISAs respectively.ConclusionThe novel MOMP_VD2-VD3 MAb-based assay may have higher specificity than the anti-EB MAb, which may possibly be used as an alternative tool for the diagnosis of C. pneumoniae infection.     

大学生抑郁情绪及相关因素的初探

社会经济的快速发展、竞争压力的增大等社会因素导致大学生的心理卫生问题明显增多。有资料说明 ,以前影响大学生学习的主要原因是躯体疾病 ,现在 ,抑郁、精神分裂症等疾病已是升为主要原因[1] 。抑郁情绪的发生可能与大学生受各种生活事件的影响、以及对这些精神刺激的应付方式有密切的关系[2 ] 。本研究对部分大学生进行了调查 ,结果如下。1　对象与方法1.1　研究对象于 2 0 0 0年 10月 ,在某综合大学进行随机抽样 ,共抽取样本 15 0 0人。其中 ,男 95 1人 (6 3.4 % )、女5 4 9人 (36 .6 % ) ;平均年龄 17～ 2 3岁 (19.3± 2 .5 5 )。大学…     

Head and neck pilomatrixoma in children.

OBJECTIVE: To provide a review of the current information on the etiology, clinical presentation, management, and outcome of pilomatrixoma of the head and neck in children. DESIGN: Retrospective review. SETTING: A tertiary care pediatric center. PATIENTS: Fifty-one pediatric patients with a diagnosis of pilomatrixoma of the head and neck. INTERVENTION: All patients underwent excision of pilomatrixoma from January 1997 to March 1999. A total of 55 tumors were studied. RESULTS: A preponderance of girls (n = 36; 71%) presented with this condition. The average age at diagnosis was 5.7 years, and the average size of the lesion was 1 cm. The skin of the cheek and the periorbital area were the most commonly involved sites. Only 27 lesions (49%) had a correct preoperative diagnosis. Two (4%) of 55 tumors recurred after complete surgical excision. MAIN OUTCOME MEASURES: The age and sex of the patient, preoperative diagnosis, time elapsed before diagnosis, site and size of the tumor, length of follow-up, presence of multiple or previous pilomatrixomas, and recurrence. CONCLUSIONS: Preoperative diagnosis may be improved with increased awareness of pilomatrixoma, a common, benign skin tumor in children. Clinical findings will aid in an accurate diagnosis. Recurrence after complete local excision is rare.     

基于IA理论的数字图书馆门户设计

结合信息构建的基本内涵和中国医学科学院医学信息研究所/图书馆"一站式"医学信息服务平台建设目标,提出"一站式"医学信息服务平台网站的设计原则,在对用户需求进行详细调研分析的基础上,介绍了平台网站界面在组织系统、导航系统、标识系统和检索系统4个方面的人性化设计实践.     

某专科医院2007年-2008年度常见细菌耐药分析

目的为临床医师合理使用抗菌药物提供参考依据,减少细菌耐药的产生。方法采用回顾性调查的方法,对北京某专科医院2007年1月-12月份从临床送检标本中培养分离的293株病原菌的种类以及对抗菌药物的敏感情况进行汇总分析。结果革兰氏阴性菌263株,占89．77％;革兰氏阳性菌30株,占10．24％。革兰氏阴性菌对六大类13种抗菌药物耐药,革兰氏阳性菌对九大类13种抗菌药物耐药。结论抗菌药物耐药形势严峻,临床医师应严格按照无菌操作规程,参照药敏试验结果,正确、合理使用抗菌药物。     

Patch testing with a textile dye mix in a baseline series in two countries

Disperse dyes are the most common contact sensitizers among textile dyes. The main aim of this study was to investigate the outcome of patch testing with a textile dye mix 6.6%. A total of 2,049 patients from Sweden and 497 from Belgium were tested with the mix, consisting of Disperse (D) Blue 35, D Yellow 3, D Orange 1 and 3, D Red 1 and 17, 1.0% each, and D Blue 106 and D Blue 124, 0.3% each. Of the total number, 65 patients, 2.6%, tested positively to the mix, 4.2% of the Belgian patients and 2.1% of the Swedish patients. Patch testing with the mix 6.6% revealed significantly more patients with contact allergy compared with testing with a previous mix 3.2% (p < 0.01). Contact allergy to the mix was significantly more common in the Belgian than in the Swedish patients.