中药葛根及其同属植物的资源利用和评价

对我国葛属（ＰｕｅｒａｒｉａＤＣ，）９种和１变种植物进行了调查研究，认为目前国内市售中药葛根的主流品种为野葛Ｐ，ｌｏｂａｔａ（Ｗｉｌｌｄ．）Ｏｈｗｉ和粉葛Ｐ．ｔｈｏｍｓｏｎｉｉ）Ｂｅｎｔｈ．。野葛在我国大部份省区都有分布，资源最为丰富；粉葛在广西载培历史悠久；其他种主要分布在西南地区，并有少数种在当地作中药葛根用。从成分分析结果看，野葛的葛根素（ｐｕｅｒａｒｉｎ）及总黄酮含量显著高于本属其它植物，粉葛次之。除野葛和粉葛外其他种均不宜作葛根药用。峨嵋葛Ｐ．ｏｍｅｉｅｎｓｉｓＷａｎｇｅｔＴａｎｇ是个有争议的种，我们比较了化学成分的含量，其结果与粉葛相近，而与山葛藤有所区别，有待进一步研究。     

Secular trend in the sexual maturation of southern Chinese boys

A random sample of 3872 southern Chinese boys ranging from 7 to 21 years of age were selected from primary and secondary schools in Hong Kong for the assessment of sexual maturation. The median ages of onset of puberty and pubic hair development were 11.4 and 12.7 years, respectively. The advancement of sexual maturation coincides with the secular increase in height and weight for Chinese children in Hong Kong.     

二阶导数紫外光谱法测定镇咳宁糖浆中盐酸麻黄碱的含量

目的:测定镇咳宁糖浆中盐酸麻黄碱的含量.方法:采用二阶导数紫外光谱法,读取盐酸麻黄碱在214.2±1nm波长吸收度.结果:线性范围为19.44-68.04μg/Ml(r=0.9993),回收率为10.2%,RSD为1.9%.结论:该方法简便准确     

口腔小唾液腺肿瘤213例回顾分析

口腔小唾液腺肿瘤（MSGTs）根据其组织形态学差异可分为多种类型，该文旨在研究MSGTs的发病率及其临床特征与组织病理学特点之间的关系。作者回顾性研究了213例MSGTs患者，所有病例均行复片证实，部分行特殊染色和免疫组化染色，并对肿瘤的临床特征进行描述。结果：119例（56％）为良性肿瘤，94例（44％）为恶性；良性肿瘤中多形性腺瘤最为常见（93／119），其次为管状腺瘤（25／119）；94例恶性肿瘤中，黏液表皮样癌45例（均为低度恶性），     

人工耳蜗植入技术的应用及手术相关解剖特点

目的:介绍人工耳蜗植入术临床应用进展以及手术相关部位的解剖特点。资料来源:应用计算机检索Medline 1986-01/2006-12与人工耳蜗植入相关的文章,检索词为“cochlear implantation,indicatio,ncomplication,dissection,electrically evoked compound action potential”,限定文献语种为“English”;同时检索万方数据库2000-01/2006-12相关文章,检索词为“人工耳蜗,适应证,并发症,解剖,电诱发的前庭蜗神经复合动作电位”,限定文献语种为中文。同时手工查找相关书籍。资料选择:共检索到相关文献300余条,进一步查找全文,选择与人工耳蜗植入术相关应用解剖、人工耳蜗植入术适应证、并发症及术后效果相关文章,纳入观察对象为人的临床研究,无论有无对照均纳入,筛除明显重复文献以及综述类文章,最后纳入40条文献进行综述。资料提炼:40条文献中研究人工耳蜗植入的解剖学文章有16篇,介绍人工耳蜗植入应用进展的文章有17篇,总结人工耳蜗植入术后调试及效果的文章有8篇。资料综合:①人工耳蜗植人术经面神经隐窝入路较理想,距圆窗龛较近,有利于电极插入,但该入路靠近面神经管,手术操作时应注意保护面神经。②随着科技进步和临床经验的积累,人工耳蜗植入的病例选择标准已放宽。耳聋的发病的原因和植入耳蜗的年龄对语后聋患者的术后听力效果影响并不明显,但对语前聋患者的人工耳蜗植入越早其疗效越好,而且在人工耳蜗植入手术前残余听力较好的患者,人工耳蜗植入手术后的效果比残余听力差者疗效要好。③内耳结构的异常影响人工耳蜗植入手术进行,但术前影像学的检查能够帮助医生做出正确判断。④人工耳蜗植入术的术后效果在很大程度上要依赖于手术后对电刺激动态范围的准确调试。结论:目前,人工耳蜗植入手术已经成为重度以上感音神经性耳聋的常规治疗手段,术前评估和术后正确调试是保证手术效果的重要措施。     

兔骨髓间充质干细胞表面抗原检测及其变化特点

目的:应用流式细胞仪对兔骨髓间充质干细胞的表面抗原进行检测,观察骨髓间充质干细胞传代次数、克隆纯化与骨髓间充质干细胞表面抗原表达之间的关系。方法:实验于2004-01/2006-08在浙江省医学科学院生物工程所完成。取3月龄新西兰大白兔用于骨髓间充质干细胞的分离培养。兔骨髓间充质干细胞的克隆化:将铺满培养瓶底的原代骨髓间充质干细胞,用D-Hanks液小心的洗1次,加入0.25%胰蛋白酶消化约4min,弃消化液,加LG-DMEM培养液收集细胞,1000r/min,离心10min,然后用LG-DMEM培养液充分混匀沉淀细胞。细胞计数后以10倍递减稀释至细胞密度为103个/mL。取0.1mL稀释后的细胞悬液加入10mL培养液,使最终细胞密度为10个/mL。将细胞悬液加入96孔培养板,每孔100μL。置37℃、体积分数为0.05的CO2饱和湿度条件下培养,每天观察克隆细胞的增殖生长状况。待克隆细胞生长至60%～80%融合时,逐步扩大培养,在液氮冻存保种的同时进行细胞连续传代。将体外普通法分离的骨髓间充质干细胞第5代、第7代、第13代、第16代、第21代、第22代及克隆纯化的骨髓间充质干细胞第3代、第5代、第15代、第26代均标记上CD14-FITC及CD44-PE,通过流式细胞仪检测其阴性率及阳性率。结果:①普通法分离的骨髓间充质干细胞各代的CD44表达呈阳性,且随着培养代次的增加,其表达的阳性率逐渐增强,到P16代以后又呈下降趋势;各代骨髓间充质干细胞表面抗原CD14出现了微弱阳性,但随着培养代次增加,其阳性率呈下降趋势。②克隆纯化的骨髓间充质干细胞其CD44呈现阳性,表达在80%以上,至P26代时CD44表达下降到70.49%;其CD14基本呈阴性。结论:兔骨髓间充质干细胞其CD44呈阳性表达,CD14呈阴性表达。随着传代代数增加CD14阴性符合率逐渐提高,CD44阳性表达率也提高。普通法分离的骨髓间充质干细胞较克隆纯化的骨髓间充质干细胞CD14阴性符合率差,前者的CD44阳性表达率也较后者低。     

Effect of the functional VEGFA-583C/T variant on vascular endothelial growth factor levels and the risk of recurrent spontaneous miscarriage

The association of vascular endothelial growth factor (VEGF) -583C/T variant with recurrent miscarriage (RSM) was investigated in 173 RSM cases and 248 control women. Increased minor allele and genotype frequencies of -583C/T, and reduced serum VEGF concentrations were associated with increased risk of RSM.     

Interleukin‐10 microsatellite variants and the risk of acute coronary syndrome among Tunisians

The objective of the study was to investigate the association of interleukin (IL)‐10 promoter microsatellite polymorphisms, linked with altered IL‐10 secretion, with the susceptibility to acute coronary syndrome (ACS) in adult Tunisian patients. We genotyped 291 ACS patients and 291 age‐, gender‐ and ethnically matched control subjects for the microsatellites IL‐10R [X78437.2g.5325CA(11_15)] and IL‐10G [X78437.2g.8134CA(14_29)] by PCR‐based assays. Haplotypes were reconstructed using maximum likelihood method. Regression analysis was used in determining the risk imparted by specific IL‐10 genotypes and haplotypes. A significant decrease in IL‐10G12 (24 CA repeats) (P < 0.001; OR = 0.465) and IL‐10G15 (27 CA repeats) (P = 0.043; OR = 0.232), and a significant increase in the low IL‐10 producer allele, IL‐10R3 (14 CA repeats) (P = 0.049; OR = 1.461), microsatellites were seen in the ACS group compared with controls. Of the possible 14 haplotypes constructed, there was an enrichment of the R2G9 (13CA vs. 21CA) haplotype in controls [P = 0.019; adjusted OR (95% CI) = 0.67 (0.48–0.94)] and R2G15 (13CA vs. 27CA) haplotype in cases [P = 0.042; adjusted OR (95% CI) = 5.29 (1.06–26.30)], thus assigning a protective and susceptible nature to these haplotypes respectively. The differential association of IL‐10 microsatellite alleles and haplotypes with ACS suggests that IL‐10 contributes to ACS pathogenesis. While the functional attributes of these microsatellite markers remain to be seen, it is likely that they have distinct functional properties (altered IL‐10 secretion), which in turn affect the susceptibility to ACS development.