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排序方式: 共有403条查询结果,搜索用时 15 毫秒
61.
Alexandr V. Shustov 《Virology》2010,400(1):8-17
In our previous studies, we have stated to build a new strategy for developing defective, pseudoinfectious flaviviruses (PIVs) and applying them as a new type of vaccine candidates. PIVs combined the efficiency of live vaccines with the safety of inactivated or subunit vaccines. The results of the present work demonstrate further development of chimeric PIVs encoding dengue virus 2 (DEN2V) glycoproteins and yellow fever virus (YFV)-derived replicative machinery as potential vaccine candidates. The newly designed PIVs have synergistically functioning mutations in the prM and NS2A proteins, which abolish processing of the latter proteins and make the defective viruses capable of producing either only noninfectious, immature and/or subviral DEN2V particles. The PIV genomes can be packaged to high titers into infectious virions in vitro using the NS1-deficient YFV helper RNAs, and both PIVs and helpers can then be passaged as two-component genome viruses at an escalating scale. 相似文献
62.
Novotna K Trkova M Pazdro A Smejkal M Soukupova A Kodetova D Smejkal P Sedlacek Z 《Digestive diseases and sciences》2006,51(1):110-113
In search of potential prognostic markers, we analyzed a large series of tissues of Barrett's esophagus and samples of adenocarcinomas
arising in the terrain of Barrett's esophagus for TP53 gene mutations by direct sequencing of exons 5 to 9 of the TP53 gene.
While 9 of 21 adenocarcinomas tested (42.9%) contained a TP53 mutation, none of 24 samples from Barrett's esophagus were mutated.
This observation suggests that TP53 gene mutation may be a relatively late event in the progression from nondysplastic Barrett's
esophagus to adenocarcinoma of esophagus. Therefore, TP53 gene mutations alone are not likely to represent a widely useful
prognostic marker of the risk of progression to malignancy, at least not in Barrett's esophagus without dysplasia.
This work was supported by grant ND/7009-3 from the Internal Grant Agency of the Ministry of Health of the Czech Republic. 相似文献
63.
Reduction of thrombogenicity of carbon nanotubes is an important prerequisite for their biomedical use. We assessed the thrombogenic activity of carboxylated single-walled carbon nanotubes (c-SWCNTs) and covalently PEGylated c-SWNCTs (PEG-SWCNTs) by testing the clotting time of platelet poor plasma and platelet aggregation in whole blood samples, and evaluated the impact of human serum albumin on thrombogenicity of carbon nanotubes. Both types of SWCNTs exhibited considerable thrombogenic activity. SWCNTs accelerated plasma clotting, with a lesser effect seen for PEG-SWCNTs. Treatment of SWCNTs with albumin did not affect the SWCNT-induced shortening of clotting time. In whole blood, no discernible differences in the effect of c-SWCNTs and PEG-SWCNTs on platelets were observed. Upon addition of SWCNTs to blood, dose- and time-dependent formation of agglomerates of nanotubes and platelets was demonstrated. Pretreatment of SWCNTs with albumin reduced the platelet aggregation: the number of single platelets increased, and the size of platelet-SWCNT agglomerates decreased dramatically. Hence, addition of albumin may serve to attenuate the adverse, thrombogenic effect of CNTs. 相似文献
64.
Svec A 《Pathology, research and practice》2008,204(11):785-792
Engagement of plasma membrane receptors is followed by an assembly of a multimolecular complex of signaling molecules organized by scaffolding or adaptor proteins. PAG is a recently characterized transmembrane adaptor protein associated with lipid rafts, which is involved in the regulation of Src-kinases, monomeric Ras protein, and interactions with the cytoskeleton. The review provides up-to-date information about the protein that attracts increasing attention in current biomedical research. 相似文献
65.
Mara K Decorosi F Viti C Giovannetti L Papaleo MC Maida I Perrin E Fondi M Vaneechoutte M Nemec A van den Barselaar M Dijkshoorn L Fani R 《Research in microbiology》2012,163(3):161-172
Characterization of bacterial communities in oil-contaminated soils and evaluation of their degradation capacities may serve as a guide for improving remediation of such environments. Using physiological and molecular methods, the aim of this work was to characterize 17 Acinetobacter strains (13 species) able to use diesel fuel oil as sole carbon and energy source. The strains were first tested for their ability to grow on different alkanes on minimal medium containing high NaCl concentrations. The envelope hydrophobicity of each strain was assessed by microbial adhesion to the hydrocarbon test (MATH) when grown in LB medium or minimal medium containing succinate or diesel fuel. Most strains were hydrophobic both in LB and minimal medium, except for strain Acinetobacter venetianus VE-C3 that was hydrophobic only in minimal medium. Furthermore, two A. venetianus strains, RAG-1(T) and LUH 7437, and strain ATCC 17905 (genomic species 13BJ) displayed biosurfactant activity. The alkM gene encoding alkane hydroxylase was detected in the chromosome of the 15 strains by PCR amplification, sequencing and Southern blot analysis. Phenotype microarray analysis performed on the five A. venetianus strains revealed that they differentially used purines as N-source and confirmed that they are unable to use carbohydrates. 相似文献
66.
67.
Machackova Tana Mlcochova Hana Stanik Michal Dolezel Jan Fedorko Michal Pacik Dalibor Poprach Alexandr Svoboda Marek Slaby Ondrej 《Tumour biology》2016,37(11):14653-14658
Tumor Biology - MicroRNAs (miRNAs) have been proven to be important oncogenes and tumor suppressors in wide range of cancers, including renal cell carcinoma (RCC). In our study, we evaluated... 相似文献
68.
Shen Y Lange O Delaglio F Rossi P Aramini JM Liu G Eletsky A Wu Y Singarapu KK Lemak A Ignatchenko A Arrowsmith CH Szyperski T Montelione GT Baker D Bax A 《Proceedings of the National Academy of Sciences of the United States of America》2008,105(12):4685-4690
Protein NMR chemical shifts are highly sensitive to local structure. A robust protocol is described that exploits this relation for de novo protein structure generation, using as input experimental parameters the (13)C(alpha), (13)C(beta), (13)C', (15)N, (1)H(alpha) and (1)H(N) NMR chemical shifts. These shifts are generally available at the early stage of the traditional NMR structure determination process, before the collection and analysis of structural restraints. The chemical shift based structure determination protocol uses an empirically optimized procedure to select protein fragments from the Protein Data Bank, in conjunction with the standard ROSETTA Monte Carlo assembly and relaxation methods. Evaluation of 16 proteins, varying in size from 56 to 129 residues, yielded full-atom models that have 0.7-1.8 A root mean square deviations for the backbone atoms relative to the experimentally determined x-ray or NMR structures. The strategy also has been successfully applied in a blind manner to nine protein targets with molecular masses up to 15.4 kDa, whose conventional NMR structure determination was conducted in parallel by the Northeast Structural Genomics Consortium. This protocol potentially provides a new direction for high-throughput NMR structure determination. 相似文献
69.
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