全文获取类型
收费全文 | 7350篇 |
免费 | 377篇 |
国内免费 | 35篇 |
专业分类
耳鼻咽喉 | 41篇 |
儿科学 | 180篇 |
妇产科学 | 161篇 |
基础医学 | 1017篇 |
口腔科学 | 131篇 |
临床医学 | 457篇 |
内科学 | 1879篇 |
皮肤病学 | 227篇 |
神经病学 | 480篇 |
特种医学 | 279篇 |
外科学 | 1310篇 |
综合类 | 17篇 |
预防医学 | 213篇 |
眼科学 | 86篇 |
药学 | 388篇 |
中国医学 | 3篇 |
肿瘤学 | 893篇 |
出版年
2023年 | 52篇 |
2022年 | 108篇 |
2021年 | 178篇 |
2020年 | 86篇 |
2019年 | 116篇 |
2018年 | 162篇 |
2017年 | 117篇 |
2016年 | 152篇 |
2015年 | 176篇 |
2014年 | 187篇 |
2013年 | 262篇 |
2012年 | 419篇 |
2011年 | 460篇 |
2010年 | 243篇 |
2009年 | 233篇 |
2008年 | 443篇 |
2007年 | 411篇 |
2006年 | 485篇 |
2005年 | 402篇 |
2004年 | 434篇 |
2003年 | 419篇 |
2002年 | 416篇 |
2001年 | 121篇 |
2000年 | 119篇 |
1999年 | 134篇 |
1998年 | 104篇 |
1997年 | 95篇 |
1996年 | 88篇 |
1995年 | 62篇 |
1994年 | 78篇 |
1993年 | 75篇 |
1992年 | 92篇 |
1991年 | 110篇 |
1990年 | 92篇 |
1989年 | 82篇 |
1988年 | 79篇 |
1987年 | 58篇 |
1986年 | 57篇 |
1985年 | 38篇 |
1984年 | 33篇 |
1983年 | 46篇 |
1982年 | 20篇 |
1981年 | 19篇 |
1980年 | 12篇 |
1979年 | 25篇 |
1978年 | 19篇 |
1977年 | 13篇 |
1976年 | 16篇 |
1971年 | 13篇 |
1965年 | 11篇 |
排序方式: 共有7762条查询结果,搜索用时 15 毫秒
71.
72.
73.
Objective: To investigate the effects of estrogen on the susceptibility to oxidation of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in postmenopausal women. Methods: A total of 23 postmenopausal women were treated with 0.625 mg of conjugated equine estrogen daily for 3 months. Blood samples were obtained before and after therapy. Plasma levels of total cholesterol and triglyceride and the concentrations of cholesterol, triglyceride, phospholipid in LDL and HDL were determined enzymatically and the levels of apolipoprotein A-I, A-II in HDL and apolipoprotein B in LDL were measured by turbidimetric immunoassay. The isolated LDL and HDL were incubated at 37°C for 24 h with CuSO4 5 μmol/l and the lipid peroxide concentration of LDL and HDL was measured. Results: Estrogen significantly reduced the plasma level of total cholesterol and significantly increased the plasma level of triglyceride. The LDL concentrations of cholesterol, phospholipid and apolipoprotein B were significantly decreased following estrogen therapy. The triglyceride level of LDL did not change significantly. The HDL concentrations of cholesterol, triglyceride, phospholipid and apolipoprotein A-I and A-II were all significantly elevated after estrogen therapy. Estrogen significantly inhibited the peroxidation of LDL at 50–2000 μg of LDL protein (14.17±4.17–11.49±1.42 nmol/200 μg of LDL protein, P<0.001) and of HDL (4.49±1.74–3.37±1.24 nmol/200 μg of HDL protein, P<0.03) induced by their incubation in the presence of CuSO4. Conclusions: Estrogen inhibited the susceptibility of LDL and HDL to oxidative modification and favorably affected lipid metabolism by reducing the number of LDL particles and increasing the number of HDL particles in plasma that were resistant to oxidation. 相似文献
74.
Takaaki Hayashi Katsuhiro Hosono Akiko Kubo Kentaro Kurata Satoshi Katagiri Kei Mizobuchi Minehiro Kurai Norihito Mamiya Mineo Kondo Toshiaki Tachibana Hirotomo Saitsu Tsutomu Ogata Tadashi Nakano Yoshihiro Hotta 《American journal of medical genetics. Part A》2020,182(6):1500-1505
Mucolipidosis type IV (MLIV) is an autosomal recessively inherited lysosomal storage disorder characterized by progressive psychomotor delay and retinal degeneration that is associated with biallelic variants in the MCOLN1 gene. The gene, which is expressed in late endosomes and lysosomes of various tissue cells, encodes the transient receptor potential channel mucolipin 1 consisting of six transmembrane domains. Here, we described 14‐year follow‐up observation of a 4‐year‐old Japanese male MLIV patient with a novel homozygous in‐frame deletion variant p.(F313del), which was identified by whole‐exome sequencing analysis. Neurological examination revealed progressive psychomotor delay, and atrophy of the corpus callosum and cerebellum was observed on brain magnetic resonance images. Ophthalmologically, corneal clouding has remained unchanged during the follow‐up period, whereas optic nerve pallor and retinal degenerative changes exhibited progressive disease courses. Light‐adapted electroretinography was non‐recordable. Transmission electron microscopy of granulocytes revealed characteristic concentric multiple lamellar structures and an electron‐dense inclusion in lysosomes. The in‐frame deletion variant was located within the second transmembrane domain, which is of putative functional importance for channel properties. 相似文献
75.
Nishiyama M Hayashi S Futsukaichi Y Suehisa E Kurata Y 《Rinsho byori. The Japanese journal of clinical pathology》2005,53(10):898-903
Platelet counts measured by automated blood cell counter often show spuriously high values when measuring samples contain particles of equal size to platelets. The major cause of spuriously high platelet counts in samples with fragmented red cells (FRC) is thought to be the FRC themselves. We studied the correlation between FRC and spuriously high platelet counts in 40 patients demonstrating FRC on blood smears. FRC were measured by manual hemocytometry and by flow cytometry using a monoclonal antibody against glycophorin A (GPA method). There was a significant correlation between spuriously high platelet counts and FRC by manual hemocytometry (r=0.60, p<0.001) or FRC by the GPA method (r=0.45, p<0.005). These data suggest that FRC are the major cause of spuriously high platelet counts in samples with FRC. 相似文献
76.
Oda Y Funasaka K Kitano M Nakama A Yoshikura T 《Environmental and molecular mutagenesis》2004,43(1):10-19
In the present study, we developed a rapid umu-microplate test system that uses the nitroreductase- and O-acetyltransferase-overproducing Salmonella typhimurium strain NM3009 and the O-acetyltransferase-overproducing S. typhimurium strain NM2009 to detect genotoxic activity in small volume samples. The assay was used to test the genotoxicity of several standard mutagens and environmental samples. Exponentially growing cultures of NM3009, NM2009, and the parental strain TA1535/pSK1002 were incubated in 96-well microplates with test chemicals both in the presence and in the absence of rat liver S9. The relative beta-galactosidase activities were then determined colorimetrically using either chlorophenol red-beta-D-galactopyranoside (CPRG) or O-nitrophenyl-beta-D-galactopyranoside (ONPG) as a measure of umuC gene induction activity. The sensitivities of NM3009 without S9 mix and NM2009 with S9 mix to nitroarenes and aromatic amines were up to 24- to 75-fold higher than those of the parent strain. Induction of umuC gene expression was detected more readily with CPRG than ONPG. The umu-microplate assay also detected genotoxicity in organic extracts of particulate matter from air samples collected in Osaka City, Japan. The pattern of the responses suggested that the genotoxic activity of the particulate extract was due primarily to nitrated polycyclic aromatic hydrocarbons. Our results indicate that the umu-microplate assay may be a useful way of carrying out rapid screens for genotoxicity in small-volume environmental samples. 相似文献
77.
N Takeda N Tamaki M Asada H Kurata S Matsumoto 《No shinkei geka. Neurological surgery》1985,13(12):1331-1334
Most of "so-called" posterior communicating artery aneurysms previously reported, originated from the internal carotid-posterior communicating junction. Aneurysms arising from the posterior communicating artery itself are very rare. The abducens nerve palsy caused by cerebral aneurysm is also very rare. We are reporting a case with the saccular aneurysm arising directly from the distal half of the posterior communicating artery presenting the abducens nerve palsy. This 73-year-old woman who had no treatment with hypertension for several years was admitted for sudden onset of severe headache, vomitting and unconsciousness on March 1, 1984. She opened her eyes when addressed and had disorientation, urinary incontinence, right-hemiparesis and left-abducens nerve palsy. A 4-vessel angiography revealed the saccular aneurysm originating directly from the distal half of the posterior communicating artery. The patient underwent left-frontotemporal craniotomy on the 27th day after subarachnoid hemorrhage under Hunt & Kosnic Grade 3. The aneurysm originated directly from the distal half of the posterior communicating artery and directed inferior-posterior-laterally below the oculomotor nerve. The neck was successfully clipped. Immediate post-operative course was uneventful until the 7th day after surgery. On the 8th day she had hypertensive intraventricular hemorrhage and expired. The autopsy could not be obtained. The saccular "true" posterior communicating artery aneurysm with isolated unilateral abducens nerve palsy as seen in our case has not been reported. Considering the operative findings, we thought the aneurysmal dome contacted directly with the abducens nerve. 相似文献
78.
Pheochromocytoma without specific symptoms 总被引:1,自引:0,他引:1
79.
80.
Makita Ayu Nagao Tomoaki Miyoshi Ken-ichi Koizumi Yohei Kurata Mie Kondo Fumikazu Shichijo Satoru Hirooka Masashi Yamaguchi Osamu 《Clinical and experimental nephrology》2021,25(9):981-987
Clinical and Experimental Nephrology - The progression of chronic kidney disease (CKD) depends on the extent of fibrosis in the kidneys; however, a renal biopsy is necessary to evaluate the... 相似文献