链球菌蛋白对人肝癌Bel-7402细胞的增殖抑制作用及其分子机制

目的:从分子水平观察链球菌蛋白质对人肝癌Bel-7402细胞的生长增殖的抑制作用。方法:实验于2005-10/2006-11在首都医科大学病原生物学系实验室完成。①链球菌32080株购自中国医学细菌保藏管理中心,人肝癌Bel-7402细胞株由首都医科大学细胞生物学系惠赠。②常规培养链球菌并按照Winters方法提取细菌蛋白。③用0,10,20,100mg/L细菌蛋白作用于单层培养的Bel-7402细胞24,48,72h,采用MTT法检测链球菌蛋白对细胞的增殖抑制作用。④Bel-7402细胞经10mg/L或50mg/L细菌蛋白分别作用48,72h后,流式细胞术检测细菌蛋白对癌细胞生长周期时相分布。⑤Bel-7402细胞经50mg/L的细菌蛋白分别作用24,48,72h后,Annexin-v PI双染法检测Bel-7402细胞凋亡率。⑥用50mg/L的链球菌蛋白分别作用于Bel-7402细胞0,24,48,72h后,Western Blot检测链球菌蛋白对Bel-7402细胞凋亡相关蛋白的影响。结果:①链球菌蛋白对Bel-7402细胞的抑制作用:链球菌蛋白能显著抑制人肝癌Bel-7402细胞增殖,100mg/L细菌蛋白作用于细胞24,48,72h的抑制率分别为15.6%,35.6%,50%,呈剂量和时间依赖关系。②细菌蛋白对肝癌细胞生长周期时相分布及细胞凋亡率的影响:经链球菌蛋白(10,50mg/L)作用后,G2/M期Bel-7402细胞比率较正常对照组增加,差异有显著性(P<0.05);流式细胞术检测显示,经50mg/L链球菌蛋白作用后,实验组Bel-7402细胞凋亡率明显高于对照组细胞(P<0.01)。③Bel-7402细胞凋亡相关蛋白的变化:Bel-7402细胞经50mg/L链球菌蛋白分别作用24,48,72h后,细胞色素C的释放逐渐增多,Procaspase-3及Bcl-2蛋白水平降低。结论:链球菌32080株蛋白对人肝癌Bel-7402细胞的生长抑制作用存在着剂量和时间依赖性,并能将细胞的生长周期阻滞于G2/M期,诱导细胞凋亡,影响Bcl-2,procaspase-3和细胞色素C等重要的凋亡相关蛋白水平。     

Gardner综合征诊治1例

结直肠息肉病合并胃息肉的女性Gardner综合征患者1例,对其先后实施了全结直肠切除、回肠袢肛管吻合、回肠造口术,造口回肠还纳术．胃镜下息肉灼除术．患者最终避免了回肠造口,能自控排便,恢复正常生活．     

食管癌术后胃食管反流和胃排空延迟的相关因素及处理

随着高位食管胃吻合数量的不断增加,食管癌术后出现胃食管反流和胃排空延迟已经成为食管外科的常见并发症,也是影响食管癌患者术后生存质量的重要因素,我们综合了近年来在此方面研究的大宗病例分析,各种手术改进方法的对比,现今食管癌临床病理学、生理学研究的基础理论以及近年来胃排空延迟治疗方面研究的新认识,从术前、手术和术后的各个环节及病理、生理等角度,分析了可能导致术后胃食管反流和胃排空延迟的各种相关因素,并总结提出了相应的处理方法.     

小藤铃儿草的生物碱成分

自小藤铃儿草Dactylicapnos torulosa(Hook.f ct Thomas.)Hutchins中分得六个单体,通过UV,IR,MS,~1HNMR和~(13)CNMR解析,鉴定了四个,其中一个为新化合物,命名为紫金龙碱(zijinlongine,Ⅰ),其他三个为已知化合物:氯化1-甲氧基小檗碱(1-methoxylberberium chloride,Ⅱ),氯化三乙基苯胺(triethylphcnylamium chloride,Ⅲ)和异紫堇定(isocorydine,Ⅳ)。Ⅱ和Ⅲ系首次从植物中得到。     

Damage Control Surgery for Abdominal Trauma

Damage control is not a modern concept, but the application of this approach represents a new paradigm in surgery, borne out of a need to save patients with severe exsanguinating injuries. Definitive control and repair may be accomplished in the immediate post injury setting but the physiological derangement due to massive shock state resulting from the severe injury and the resuscitation that follows, often leads to a fully repaired but dead patient. The vicious triad of death in trauma, namely hypothermia, acidosis and coagulopathy should be tackled by initial abbreviated laparotomy, correction of physiological derangements and finally definitive repair of all injuries at second laparotomy. The concept needs a dedicated team effort with careful patient selection for achieving the optimal results.Key Words: Damage control surgery, hypothermia, acidosis, coagulopathy     

Declining prevalence of dental caries in school children in Singapore

OBJECTIVE: Dental health surveys of school children in Singapore from past decades were reviewed to document reductions in prevalence of dental caries.
MATERIALS AND METHODS: Surveys were carried out in schools by the Dental Division, Ministry of Health in 1970, 1979, 1984, 1989 and 1994.A sample size of approximately 5000 school children representing 1.2% of the school population aged 6 to 18 years old was examined in each survey.
RESULTS: Survey data showed an increase in the proportion of children free of caries in the permanent dentition from 30% in 1970 to 58.7% in 1994. The DMFT index for 6 to 18 year old children has dropped from 2.98 in 1970, 2.61 in 1979, 1.97 in 1984, 1.61 in 1989 to 1.05 in 1994.
CONCLUSION: Fluoridation of public water supplies together with the adoption of preventive dental health programmes by the State and professional bodies is credited as the major factor in caries reduction.     

Tracheobronchial stenosis in infants: successful balloon dilation therapy

Four infants with congenital or acquired tracheobronchial stenosis were successfully treated with angioplasty balloon catheter dilation. The technical details and complications of these procedures are described. The authors believe balloon dilation therapy should be considered as the initial form of therapy for tracheal stenosis in infants, even in the presence of complex stenotic lesions.