Subtraction MR Venography Acquired from Time-Resolved Contrast-Enhanced MR Angiography: Comparison with Phase-Contrast MR Venography and Single-Phase Contrast-Enhanced MR Venography

ObjectiveTo evaluate the image characteristics of subtraction magnetic resonance venography (SMRV) from time-resolved contrast-enhanced MR angiography (TRMRA) compared with phase-contrast MR venography (PCMRV) and single-phase contrast-enhanced MR venography (CEMRV).ResultsSingle-phase contrast-enhanced MR venography showed better image quality (median score 4 in both reviewers) than did the other two MRVs (p < 0.001), whereas SMRV (median score 3 in both reviewers) and PCMRV (median score 3 in both reviewers) had similar image quality (p ≥ 0.951). SMRV (median score 0 in both reviewers) suppressed arterial signal better than did the other MRVs (median score 1 in CEMRV, median score 2 in PCMRV, both reviewers) (p < 0.001). The dural sinus score of SMRV (median and interquartile range [IQR] 48, 43-50 for reviewer 1, 47, 43-49 for reviewer 2) was significantly higher than for PCMRV (median and IQR 31, 25-34 for reviewer 1, 30, 23-32 for reviewer 2) (p < 0.01) and did not differ from that of CEMRV (median and IQR 50, 47-52 for reviewer 1, 49, 45-51 for reviewer 2) (p = 0.146 in reviewer 1 and 0.123 in reviewer 2). The SNR and CNR of SMRV (median and IQR 104.5, 83.1-121.2 and 104.1, 74.9-120.5, respectively) were between those of CEMRV (median and IQR 150.3, 111-182.6 and 148.4, 108-178.2) and PCMRV (median and IQR 59.4, 49.2-74.9 and 53.6, 43.8-69.2).ConclusionSubtraction magnetic resonance venography is a promising MRV method, with acceptable image quality and good arterial suppression.     

针对性护理对重度烧伤伴并发症患者负性情绪及生活质量的影响

目的 分析针对性护理对重度烧伤伴并发症患者负性情绪及生活质量的影响。方法 选取邯郸钢 铁集团有限责任公司职工医院2020年6月-2022年9月收治的104例重度烧伤伴并发症患者为研究对象,依据 随机数字表法分为观察组与对照组,各52例。对照组行常规护理干预,观察组实施针对性护理干预,比较 两组负性情绪、生活质量及护理满意度。结果 两组护理后SAS、SDS评分均低于护理前,且观察组低于对 照组,差异有统计学意义（P＜0.05）;两组护理后躯体功能、社会关系、心理功能、一般健康状况评分均 高于护理前,且观察组高于对照组,差异有统计学意义（P＜0.05）;观察组护理满意度高于对照组,差异 有统计学意义（P＜0.05）。结论 对重度烧伤伴并发症发生的患者实施针对性护理干预可减轻其抑郁焦虑 情绪,提升生活质量,促进护理满意度的提高。     

针对性护理对重度烧伤伴并发症患者创面恢复及生存质量的影响

目的 分析针对性护理对重度烧伤伴并发症患者创面恢复及生存质量的影响。方法 选取邯郸 钢铁集团有限责任公司职工医院烧伤整形科2021年6月-2022年9月收治的68例重度烧伤伴并发症患者为 研究对象,依据随机数字表法分为观察组与对照组,各34例。对照组行常规护理干预,观察组给予针对 性护理,比较两组护理干预后疼痛情况（VAS评分）、创面恢复情况（愈合时间及愈合率）、生活质量 （BSHS-A量表评分）。结果 观察组干预后VAS评分低于对照组,愈合时间及愈合率优于对照组,差异 有统计学意义（P＜0.05）;两组干预后躯体功能、社会关系、心理功能、一般健康状况评分均高于干预 前,且观察组高于对照组,差异有统计学意义（P＜0.05）。结论 对重度烧伤伴并发症的患者实施针对 性护理干预可缩短创面愈合时间,促进创面愈合,同时可提高患者生活质量,减轻疼痛程度,值得临床 应用。     

Intraoperative fluid administration volumes during pediatric liver transplantation and postoperative outcomes: A multicenter analysis

Introduction

Fluid administration is an important aspect of the management of children undergoing liver transplantation and may impact postoperative outcomes. Our aim was to evaluate the association between volume of intraoperative fluid administration and our primary outcome, the duration of postoperative mechanical ventilation following pediatric liver transplantation. Secondary outcomes included intensive care unit length of stay and hospital length of stay.

Methods

We conducted a multicenter, retrospective cohort study using electronic data from three major pediatric liver transplant centers. Intraoperative fluid administration was indexed to weight and duration of anesthesia. Univariate and stepwise linear regression analyses were conducted.

Results

Among 286 successful pediatric liver transplants, the median duration of postoperative mechanical ventilation was 10.8 h (IQR 0.0, 35.4), the median intensive care unit length of stay was 4.3 days (IQR 2.7, 6.8), and the median hospital length of stay was 13.6 days (9.8, 21.1). Univariate linear regression showed a weak correlation between intraoperative fluids and duration of ventilation (r² = .037, p = .001). Following stepwise linear regression, intraoperative fluid administration remained weakly correlated (r² = .161, p = .04) with duration of postoperative ventilation. The following variables were also independently correlated with duration of ventilation: center (Riley Children's Health versus Children's Health Dallas, p = .001), and open abdominal incision after transplant (p = .001).

Discussion

The amount of intraoperative fluid administration is correlated with duration of postoperative mechanical ventilation in children undergoing liver transplantation, however, it does not seem to be a strong factor.

Conclusions

Other modifiable factors should be sought which may lead to improved postoperative outcomes in this highly vulnerable patient population.     

Prognostic Value of Metabolic Tumor Volume Estimated by 18 F-FDG Positron Emission Tomography/Computed Tomography in Patients with Diffuse Large B-Cell Lymphoma of Stage II or III Disease

Purpose

The purpose of this study was to evaluate the prognostic value of metabolic tumor volume (MTV) measured by ¹⁸F-fluorodeoxyglucose positron emission tomography-computed tomography (FDG-PET/CT) in patients with diffuse large B-cell lymphoma (DLBCL) treated with rituximab-containing immunochemotherapy.

Methods

Patients with newly diagnosed DLBCL who underwent pre-treatment torso FDG-PET/CT scan taken within 10 days before treatment were included. MTV was defined as the volume of hypermetabolic tissue with a standardized uptake value (SUV) greater than a threshold value of 2.5 and calculated using volume viewer software. Association of MTV with patient characteristics and survival were compared.

Results

A total of 96 patients were evaluated. During a median follow-up period of 27.8 months, 3-year event-free survival (EFS) and overall survival was 69.5 % and 72.9 %, respectively. The Ann Arbor staging showed a limitation of prognosis because there was no difference of EFS between patients with Ann Arbor stage II and those with stage III. On the contrary, among patients with Ann Arbor stage II or III disease (n?=?53), the higher MTV group showed significantly inferior EFS compared with the lower MTV group.

Conclusions

In the current study, we identified the pre-treatment MTV measured by FDG-PET/CT as a potential predictor of survival in patients with DLBCL treated with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisolone (R-CHOP), at least in Ann Arbor stage II and III disease.     

PCGEM1 stimulates proliferation of osteoarthritic synoviocytes by acting as a sponge for miR‐770

Long non‐coding RNAs (lncRNAs) have been reported to play important roles in cellular metabolism and development. Various diseases have been associated with aberrant expression of lncRNAs and the related dysregulation of mRNAs. An lncRNA profiling assay was carried out to identify the key lncRNA in osteoarthritic human synoviocytes; the results revealed that prostate cancer gene expression marker 1 (PCGEM1) was significantly overexpressed in osteoarthritic synoviocytes. Exogenous overexpression of PCGEM1 inhibited apoptosis, induced autophagy, and stimulated the proliferation of human synoviocytes. The increased expression of PCGEM1 in human synoviocytes also suppressed the expression of miR‐770. Transfection of the miR‐770 precursor resulted in reduced proliferation, and induced apoptosis of human synoviocytes. This effect of miR‐770 expression was reversed by co‐introduction of PCGEM1. Target validation showed a direct binding between PCGEM1 and miR‐770. We demonstrate that PCGEM1 act as sponge lncRNA for miR‐770 that regulates proliferation/apoptosis and autophagy, and suggest PCGEM1 as possible target for OA therapy. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:412–418, 2016.     

Expression of cubilin in mouse testes and Leydig cells

Cubilin (cubn) is a receptor for vitamins and various protein ligands. Cubn lacks a transmembrane domain but anchors to apical membranes by forming complexes with Amnionless or Megalin. In an effort to better understand the uptake of nutrients in testis, we analysed cubn expression in the developing mice testes. In testes, cubn mRNA increased from birth to adulthood. In the inter‐stitium and isolated seminiferous tubules, neonatal increase in cubn mRNA until 14 days post‐partum (pp) was followed by a marked increase at puberty (28 days pp). Cubn was found in the gonocytes, spermatogonia, spermatocytes and spermatids in the developing testes. In adult testes, strong Cubn immunoreactivity was found in the elongating spermatids, suggesting the role of Cubn in endocytosis during early spermiogenesis. In Sertoli cells and peritubular cells, Cubn immunoreactivity was weak throughout the testis development. In the inter‐stitium, Cubn immunoreactivity was found in foetal Leydig cells, was weak to negligible in the stem cells and progenitor Leydig cells and was strong in immature and adult Leydig cells, demonstrating a positive association between Cubn and steroidogenic activity of Leydig cells. Collectively, these results suggest that Cubn may participate in the endocytotic uptake of nutrients in germ cells and somatic cells, supporting the spermatogenesis and steroidogenesis in mouse testes.     

伯氨喹与蒿甲醚联合治疗恶性疟疾的疗效评价

目的评价伯氨喹与蒿甲醚联合治疗恶性疟疾的疗效。方法选择2013年5月—2014年3月坦桑尼亚中坦友谊医院住院的恶性疟疾100例,按设定的治疗策略随机分为治疗组和对照组各50例。对照组:肌内注射80 mg蒿甲醚,1 d-1,连续治疗7 d,首次剂量160 mg;治疗组:在对照组的基础上,加服22.5 mg伯氨喹,1 d-1,连续服用8 d。依据恶性疟疾临床治愈标准,评价2组患者出院时疗效(痊愈、无效)并观察治疗期间的不良反应和治愈患者出院后28 d内的复发情况。结果治疗组和对照组痊愈、无效、复发率分别为98%、0、2%和82%、4%、14%,差异有统计学意义(P<0.05);总不良反应发生率分别为8%、16%,2组间差异有统计学意义(P<0.05)。结论伯氨喹与蒿甲醚联合治疗可以提高恶性疟疾疗效,降低复发率,不良反应更少。     

区域CT值在预测股骨近端骨折中的价值

目的:探讨60岁以上成人发生股骨近端骨折的股骨颈部松质骨CT值。方法:自2020年1月至2020年12月,回顾性分析行双髋关节CT检查的年龄＞60岁的280例研究对象,男85例,女195例;年龄75(66,82)岁;左侧120例,右侧160例。其中136例股骨近端骨折患者为研究组,144例无骨折者为对照组。采用GEOptima CT机扫描并分别重建出股骨近端水平位、冠状位和矢状位层面。测量并比较两组股骨颈部松质骨的CT值;采用Logistic回归分析股骨颈松质骨CT值与股骨颈骨折间的关系。结果:骨折组年龄79(73.3,85.0)岁,无骨折组年龄69.5(64.0,78.8)岁,两组年龄比较,差异有统计学意义(P＜0.05)。骨折组区域CT值8.62(-3.62,27.15) HU低于无骨折组34.31(-5.93,71.74) HU(P＜0.05)。冠状位平面内骨折组的区域CT值-8.48(-30.96,17.46) HU低于无骨折组40.49(5.55,80.71) HU(P＜0.05);矢状位平面内骨折组区域CT值-31.28(-54.91,-5.11) HU低于无骨折组7.74(-20.12,44.54) HU(P＜0.05);水平位平面内骨折组区域CT值0.17(-23.13,24.60) HU低于无骨折组46.40(10.42,85.18) HU(P＜0.05);骨折组股骨颈部区域CT值低于无骨折组(P＜0.05)。经Logistic回归分析,冠状位区域CT值是股骨近端骨折的影响因素,可以写入预测骨折概率的回归方程。结论:在60岁以上成人,随着年龄的不断增大,股骨颈部松质骨CT值呈下降趋势。股骨颈部松质骨CT值越小发生股骨近端骨折的风险越大。     

Comparing MR imaging and CT in the staging of gastric carcinoma

OBJECTIVE: The purpose of this study was to assess the usefulness of breath-hold two-dimensional (2D) fast low-angle shot (FLASH) and T2-weighted turbo spin-echo fast MR imaging compared with helical CT in the staging of gastric carcinoma. SUBJECTS AND METHODS: Thirty patients with gastric carcinoma underwent preoperative MR imaging and helical CT. MR imaging at 1.5 T was performed immediately after the intramuscular injection of scopolamine and the oral administration of water or effervescent granules. Breath-hold 2D FLASH T1-weighted images in all three planes, turbo spin-echo T2-weighted axial images, and gadolinium-enhanced fat-suppressed 2D FLASH axial images were included. Helical CT was performed 60 sec after initiation of i.v. contrast medium injection (2.5-3 ml/sec). Two groups of two radiologists each independently analyzed the MR and helical CT findings, and these results were compared with the pathologic findings. RESULTS: For T staging, MR imaging accuracy was higher than that of helical CT (73.3% and 66.7%, respectively); however, the accuracies of the two methods were not significantly different from each other (McNemar test, p > 0.05). Overstaging was noted in 6.7% of cases with MR imaging and 10% with helical CT. Understaging was noted in 20% of cases with MR imaging and 23.3% with helical CT. For N staging, the accuracies of MR imaging and helical CT were 55% and 58.6%, respectively, with no statistical significance (overstaging, 10% and 6.9%; understaging, 34.5% and 34.5%, respectively). CONCLUSION: MR imaging was comparable to helical CT in the T and N staging of gastric cancer.